Michael J. Getz
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Cryptic MCAT Enhancer Regulation in Fibroblasts and Smooth Muscle Cells Open
An asymmetric polypurine-polypyrimidine cis-element located in the 5' region of the mouse vascular smooth muscle alpha-actin gene serves as a binding site for multiple proteins with specific affinity for either single- or double-stranded D…
Altered Sensitivity to Single-strand-specific Reagents Associated with the Genomic Vascular Smooth Muscle α-Actin Promoter during Myofibroblast Differentiation Open
Stimulation of quiescent AKR-2B mouse fibroblasts with transforming growth factor beta1 results in uniform conversion to a myofibroblast-like phenotype as judged by a rapid accumulation of smooth muscle alpha-actin mRNA and protein. Becaus…
The single-stranded DNA-binding proteins, Purα, Purβ, and MSY1, specifically interact with an exon 3-derived mouse vascular smooth muscle α-actin messenger RNA sequence. Open
Page 38272, Figs. 5 and 6 legends: Proteins were resolved on 13% SDS-polyacrylamide gels, not 10%. Page 38274, Fig. 9 legend: Several words were omitted. The corrected legend should read: “Fig. 9. Amino acids 264–324 are required for Purβ …
The Single-stranded DNA-binding Proteins, Purα, Purβ, and MSY1 Specifically Interact with an Exon 3-derived Mouse Vascular Smooth Muscle α-Actin Messenger RNA Sequence Open
Amino acids 44-53 of mouse vascular smooth muscle alpha-actin are encoded by a region of exon 3 that bears structural similarity to an essential MCAT enhancer element in the 5' promoter of the gene. The single-stranded DNA-binding proteins…
Molecular Interactions between Single-stranded DNA-binding Proteins Associated with an Essential MCAT Element in the Mouse Smooth Muscle α-Actin Promoter Open
Transcriptional activity of the mouse vascular smooth muscle alpha-actin gene in fibroblasts is regulated, in part, by a 30-base pair asymmetric polypurine-polypyrimidine tract containing an essential MCAT enhancer motif. The double-strand…
Sequence of cDNAs Encoding Components of Vascular Actin Single-stranded DNA-binding Factor 2 Establish Identity to Purα and Purβ Open
Transcriptional repression of the mouse vascular smooth muscle alpha-actin gene in fibroblasts and myoblasts is mediated, in part, by the interaction of two single-stranded DNA binding activities with opposite strands of an essential trans…
Repression of Transcriptional Enhancer Factor-1 and Activator Protein-1-dependent Enhancer Activity by Vascular Actin Single-stranded DNA Binding Factor 2 Open
Transcriptional repression of the murine vascular smooth muscle alpha-actin gene in fibroblasts results from the interaction of two sequence-specific single-stranded DNA binding activities (VACssBF1 and VACssBF2) with opposite strands of a…
Negative Regulation of the Vascular Smooth Muscle α-Actin Gene in Fibroblasts and Myoblasts: Disruption of Enhancer Function by Sequence-Specific Single-Stranded-DNA-Binding Proteins Open
Transcriptional activation and repression of the vascular smooth muscle (VSM) alpha-actin gene in myoblasts and fibroblasts is mediated, in part, by positive and negative elements contained within an approximately 30-bp polypurine-polypyri…
Plasticity of Vascular Smooth Muscle α-Actin Gene Transcription Open
Transcriptional activity of the mouse vascular smooth muscle (VSM) alpha-actin promoter was governed by both cell type and developmental stage-specific mechanisms. A purine-rich motif (PrM) located as -181 to -176 in the promoter was absol…
Activation of c-fos gene expression by a kinase-deficient epidermal growth factor receptor. Open
The intrinsic tyrosine kinase activity of the epidermal growth factor receptor (EGFR) has been shown to be responsible for many of the pleiotropic intracellular effects resulting from ligand stimulation [W.S. Chen, C.S. Lazar, M. Poenie, R…
Positive and negative cis-acting regulatory elements mediate expression of the mouse vascular smooth muscle alpha-actin gene. Open
Segments of the 5'-flanking region of the mouse vascular smooth muscle alpha-actin gene were assayed for promoter activity in transfected mouse BC3H1 myogenic cells and AKR-2B embryonic fibroblasts. The region between -150 and -191 that fu…
Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type beta 1. Open
We have cloned a serum- and cycloheximide-inducible mRNA from AKR-2B murine fibroblasts which encodes a protein with significant sequence similarity to human tissue factor, a cellular initiator of the blood coagulation cascade. Information…
Cooperative stimulation of specific gene transcription by epidermal growth factor and transforming growth factor type beta 1. Open
Transforming growth factor type beta 1 (TGF-beta 1) is a pleiotropic regulator of cell growth and differentiation which can potentiate or otherwise modify cellular responses to different growth factors such as epidermal growth factor (EGF)…
Induction of fibronectin gene transcription and mRNA is a primary response to growth-factor stimulation of AKR-2B cells. Open
A cDNA library, prepared from poly(A)+ RNA isolated from quiescent AKR-2B cells 4 hr after stimulation with epidermal growth factor in the presence of cycloheximide, was screened to identify RNA transcripts whose abundance is specifically …
Evidence that the Functional β-Actin Gene Is Single Copy in Most Mice and Is Associated with 5′ Sequences Capable of Conferring Serum- and Cycloheximide-Dependent Regulation Open
Hybridization to synthetic oligonucleotides representing conserved regions in the promoter and first intron of several vertebrate beta-actin genes was used to discriminate between what appears to be a single functional beta-actin gene and …
Specific stimulation of actin gene transcription by epidermal growth factor and cycloheximide. Open
Stimulation of quiescent AKR-2B mouse embryo cells with epidermal growth factor (EGF) results in a rapid and specific induction of actin mRNA sequences. These mRNAs include those coding for both beta- and gamma-cytoskeletal, but not alpha-…
Structure and expression of mouse VL30 genes. Open
DNA sequencing and blot hybridization analyses have been used to study the structure of a mouse VL30 gene and the molecular nature of VL30-related RNA which is induced upon the stimulation of cultured AKR mouse embryo cells with defined pe…
Discrete regions of sequence homology between cloned rodent VL30 genetic elements and AKV related MuLV provirus genomes Open
Southern blot analyses using reduced stringency hybridization conditions have been employed to search for sequence homologies between rodent VL30 genes and murine leukemia virus (MuLV) proviruses. These constitute two classes of transposon…
Evidence for an early evolutionary origin and locus polymorphism of mouse VL30 DNA sequences Open
The VL30 sequences of mouse DNA are a family of sequences with retrovirus-like structure which code for a 30S RNA transcript that can be packaged into the virions of murine leukemia viruses and thereby transmitted from cell to cell. A Sout…
The concept of mRNA abundance classes: a critical reevaluation Open
Derivative plots have been constructed for hybridization reactions between polysomal poly(A)-containing RNA and oligo(dT)-primed cDNA. In one method the derivative was calculated directly from the data, and in the other, from a non-linear …
<sup>125</sup> I-Labeled DNA·RNA Hybrids in Cytological Preparations Open
RNA complementary to bulk humanplacental DNA was synthesized in vitro both in the presence and absence of 3 H-labeled ribonucleotides. The 3 H-labeled RNA was used directly for hybridization to the DNA of human metaphase chromosomes, where…