Michael J. Dark
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View article: Spatial transcriptomic sequencing of fresh frozen canine osteosarcoma tumor samples
Spatial transcriptomic sequencing of fresh frozen canine osteosarcoma tumor samples Open
Osteosarcoma (OSA) is the most common malignant bone tumor in dogs and serves as a translational model for human pediatric OSA due to shared molecular features. This aggressive and metastatic cancer exhibits significant heterogeneity, whic…
View article: A novel proficiency test to assess the animal diagnostic investigation process in identifying an unknown toxicant
A novel proficiency test to assess the animal diagnostic investigation process in identifying an unknown toxicant Open
Participation in Proficiency Tests (PTs) is an important component of quality assurance in testing laboratories. In a typical chemistry PT, blind-coded samples are sent to participants for analysis of specific chemical agents, and results …
View article: Nuclear pleomorphism in canine cutaneous mast cell tumors: Comparison of reproducibility and prognostic relevance between estimates, manual morphometry, and algorithmic morphometry
Nuclear pleomorphism in canine cutaneous mast cell tumors: Comparison of reproducibility and prognostic relevance between estimates, manual morphometry, and algorithmic morphometry Open
Variation in nuclear size and shape is an important criterion of malignancy for many tumor types; however, categorical estimates by pathologists have poor reproducibility. Measurements of nuclear characteristics can improve reproducibility…
View article: Information mismatch in PHH3-assisted mitosis annotation leads to interpretation shifts in H&E slide analysis
Information mismatch in PHH3-assisted mitosis annotation leads to interpretation shifts in H&E slide analysis Open
The count of mitotic figures (MFs) observed in hematoxylin and eosin (H&E)-stained slides is an important prognostic marker, as it is a measure for tumor cell proliferation. However, the identification of MFs has a known low inter-rater ag…
View article: Information Mismatch in PHH3-Assisted Mitosis Annotation Leads to Interpretation Shifts in H&E Slide Analysis
Information Mismatch in PHH3-Assisted Mitosis Annotation Leads to Interpretation Shifts in H&E Slide Analysis Open
The count of mitotic figures (MFs) observed in hematoxylin and eosin (H&E)-stained slides is an important prognostic marker, as it is a measure for tumor cell proliferation. However, the identification of MFs has a known low inter-rater ag…
View article: Automated Nuclear Morphometry: A Deep Learning Approach for Prognostication in Canine Pulmonary Carcinoma to Enhance Reproducibility
Automated Nuclear Morphometry: A Deep Learning Approach for Prognostication in Canine Pulmonary Carcinoma to Enhance Reproducibility Open
The integration of deep learning-based tools into diagnostic workflows is increasingly prevalent due to their efficiency and reproducibility in various settings. We investigated the utility of automated nuclear morphometry for assessing nu…
View article: Keeping Pathologists in the Loop and an Adaptive F1-Score Threshold Method for Mitosis Detection in Canine Perivascular Wall Tumours
Keeping Pathologists in the Loop and an Adaptive F1-Score Threshold Method for Mitosis Detection in Canine Perivascular Wall Tumours Open
Performing a mitosis count (MC) is the diagnostic task of histologically grading canine Soft Tissue Sarcoma (cSTS). However, mitosis count is subject to inter- and intra-observer variability. Deep learning models can offer a standardisatio…
View article: Nuclear Pleomorphism in Canine Cutaneous Mast Cell Tumors: Comparison of Reproducibility and Prognostic Relevance between Estimates, Manual Morphometry and Algorithmic Morphometry
Nuclear Pleomorphism in Canine Cutaneous Mast Cell Tumors: Comparison of Reproducibility and Prognostic Relevance between Estimates, Manual Morphometry and Algorithmic Morphometry Open
Variation in nuclear size and shape is an important criterion of malignancy for many tumor types; however, categorical estimates by pathologists have poor reproducibility. Measurements of nuclear characteristics (morphometry) can improve r…
View article: Multiple biliary duct hamartomas in a cat resulting in a hepatic mass: A case report
Multiple biliary duct hamartomas in a cat resulting in a hepatic mass: A case report Open
A 13‐year‐old, male neutered domestic short‐haired cat was diagnosed with multiple biliary duct hamartomas after liver lobectomy for a suspected malignant hepatic mass. Distinguishing ultrasonographic findings included a lobular, mostly we…
View article: Chronic intermittent dyschezia in a 10-year-old spayed female domestic shorthair cat
Chronic intermittent dyschezia in a 10-year-old spayed female domestic shorthair cat Open
A 10-year-old 4.3-kg spayed female domestic shorthair cat presented to the primary veterinarian for a 1-month history of intermittent dyschezia.Physical examination findings at that time were unremarkable, except for palpation of a moderat…
View article: Detection of Necrosis in Digitised Whole-Slide Images for Better Grading of Canine Soft-Tissue Sarcomas Using Machine-Learning
Detection of Necrosis in Digitised Whole-Slide Images for Better Grading of Canine Soft-Tissue Sarcomas Using Machine-Learning Open
The definitive diagnosis of canine soft-tissue sarcomas (STSs) is based on histological assessment of formalin-fixed tissues. Assessment of parameters, such as degree of differentiation, necrosis score and mitotic score, give rise to a fin…
View article: Congenital hypospadias and vertebral malformation in a Barbados Blackbelly neonate
Congenital hypospadias and vertebral malformation in a Barbados Blackbelly neonate Open
A male purebred Barbados Blackbelly, twin to another male, was born with obvious external congenital abnormalities. Male twinwas normal. Abnormal lamb had a short torso and spinal column. At 1 week, the lamb was presented with dyspnea and …
View article: International Guidelines for Veterinary Tumor Pathology: A Call to Action
International Guidelines for Veterinary Tumor Pathology: A Call to Action Open
Standardization of tumor assessment lays the foundation for validation of grading systems, permits reproducibility of oncologic studies among investigators, and increases confidence in the significance of study results. Currently, there is…
View article: SDHB and SDHA Immunohistochemistry in Canine Pheochromocytomas
SDHB and SDHA Immunohistochemistry in Canine Pheochromocytomas Open
Pheochromocytomas (PCs) are tumors arising from the chromaffin cells of the adrenal glands and are the most common tumors of the adrenal medulla in animals. In people, these are highly correlated to inherited gene mutations in the succinat…
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Issue Information Open
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View article: Complete Urogenital and Anorectal Duplication in a Dog
Complete Urogenital and Anorectal Duplication in a Dog Open
A 10-week-old sexually intact female golden retriever was evaluated for two functional anal openings and a bipartite vulva. Examination revealed haired skin between two separate anatomically smaller anal openings. On rectal palpation, a so…
View article: Immunogenicity and Efficacy of a Novel Multi-Antigenic Peptide Vaccine Based on Cross-Reactivity between Feline and Human Immunodeficiency Viruses
Immunogenicity and Efficacy of a Novel Multi-Antigenic Peptide Vaccine Based on Cross-Reactivity between Feline and Human Immunodeficiency Viruses Open
For the development of an effective HIV-1 vaccine, evolutionarily conserved epitopes between feline and human immunodeficiency viruses (FIV and HIV-1) were determined by analyzing overlapping peptides from retroviral genomes that induced b…
View article: Viral Enrichment Methods Affect the Detection but Not Sequence Variation of West Nile Virus in Equine Brain Tissue
Viral Enrichment Methods Affect the Detection but Not Sequence Variation of West Nile Virus in Equine Brain Tissue Open
West Nile virus (WNV), a small, positive sense, single stranded RNA virus continues to encroach into new locales with emergence of new viral variants. Neurological disease in the equine can be moderate to severe in the face of low to undet…
View article: Gene Therapy for Osteoarthritis: Pharmacokinetics of Intra-Articular Self-Complementary Adeno-Associated Virus Interleukin-1 Receptor Antagonist Delivery in an Equine Model
Gene Therapy for Osteoarthritis: Pharmacokinetics of Intra-Articular Self-Complementary Adeno-Associated Virus Interleukin-1 Receptor Antagonist Delivery in an Equine Model Open
Toward the treatment of osteoarthritis (OA), the authors have been investigating self-complementary adeno-associated virus (scAAV) for intra-articular delivery of therapeutic gene products. As OA frequently affects weight-bearing joints, p…
View article: Self-Complementary Adeno-Associated Virus–Mediated Interleukin-1 Receptor Antagonist Gene Delivery for the Treatment of Osteoarthritis: Test of Efficacy in an Equine Model
Self-Complementary Adeno-Associated Virus–Mediated Interleukin-1 Receptor Antagonist Gene Delivery for the Treatment of Osteoarthritis: Test of Efficacy in an Equine Model Open
The authors are investigating self-complementary adeno-associated virus (scAAV) as a vector for intra-articular gene-delivery of interleukin-1 receptor antagonist (IL-1Ra), and its therapeutic capacity in the treatment of osteoarthritis (O…
View article: Histologic characterization of eosinophilic encephalitis in horses in Florida
Histologic characterization of eosinophilic encephalitis in horses in Florida Open
Eosinophils within the central nervous system are abnormal and are usually associated with fungal or parasitic infections in horses. Causative agents include Halicephalobus gingivalis, Sarcocystis neurona, and Neospora hughesi. Confirmatio…
View article: A retrospective study of the prevalence of calcium oxalate crystals in veterinary <i>Aspergillus</i> cases
A retrospective study of the prevalence of calcium oxalate crystals in veterinary <i>Aspergillus</i> cases Open
Fungi in the genus Aspergillus are some of the most common fungal pathogens in veterinary species, primarily affecting the respiratory tract. In both human and veterinary cases, calcium oxalate crystals have been documented in sites of Asp…
View article: Determining the utility of veterinary tissue archives for retrospective DNA analysis
Determining the utility of veterinary tissue archives for retrospective DNA analysis Open
Histopathology tissue archives can be an important source of specimens for retrospective studies, as these include samples covering a large number of diseases. In veterinary medicine, archives also contain samples from a large variety of s…
View article: Peer Review #2 of "Determining the utility of veterinary tissue archives for retrospective DNA analysis (v0.2)"
Peer Review #2 of "Determining the utility of veterinary tissue archives for retrospective DNA analysis (v0.2)" Open
Histopathology tissue archives can be an important source of specimens for retrospective studies, as these include samples covering a large number of diseases.In veterinary medicine, archives also contain samples from a large variety of sp…
View article: Peer Review #1 of "Determining the utility of veterinary tissue archives for retrospective DNA analysis (v0.1)"
Peer Review #1 of "Determining the utility of veterinary tissue archives for retrospective DNA analysis (v0.1)" Open
Histopathology tissue archives can be an important source of specimens for retrospective studies, as these include samples covering a large number of diseases.In veterinary medicine, archives also contain samples from a large variety of sp…
View article: Peer Review #1 of "Determining the utility of veterinary tissue archives for retrospective DNA analysis (v0.2)"
Peer Review #1 of "Determining the utility of veterinary tissue archives for retrospective DNA analysis (v0.2)" Open
Histopathology tissue archives can be an important source of specimens for retrospective studies, as these include samples covering a large number of diseases.In veterinary medicine, archives also contain samples from a large variety of sp…
View article: Peer Review #2 of "Determining the utility of veterinary tissue archives for retrospective DNA analysis (v0.1)"
Peer Review #2 of "Determining the utility of veterinary tissue archives for retrospective DNA analysis (v0.1)" Open
Histopathology tissue archives can be an important source of specimens for retrospective studies, as these include samples covering a large number of diseases.In veterinary medicine, archives also contain samples from a large variety of sp…
View article: Superinfection Exclusion of the Ruminant Pathogen Anaplasma marginale in Its Tick Vector Is Dependent on the Time between Exposures to the Strains
Superinfection Exclusion of the Ruminant Pathogen Anaplasma marginale in Its Tick Vector Is Dependent on the Time between Exposures to the Strains Open
The remarkable genetic diversity of vector-borne pathogens allows for the establishment of superinfection in the mammalian host. To have a long-term impact on population strain structure, the introduced strains must also be transmitted by …