Michael J. Smanski
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View article: Improving Perennial Ryegrass Transformation Protocols with Developmental Regulators
Improving Perennial Ryegrass Transformation Protocols with Developmental Regulators Open
The development of genetically modified (GM) or gene edited (GE) turfgrass requires transformation systems that are both efficient and broadly applicable across genotypes. However, traditional Agrobacterium -mediated callus culture methods…
View article: Biosynthesis of tauro-ursodeoxycholic acid (TUDCA) in Saccharomyces cerevisiae
Biosynthesis of tauro-ursodeoxycholic acid (TUDCA) in Saccharomyces cerevisiae Open
Tauroursodeoxycholic acid (TUDCA) is a microbial bile acid known for its diverse yet still largely unexplored biological activities. Traditionally used in Asian medicine, TUDCA and the taurine-free form, ursodeoxycholic acid (UDCA), share …
View article: Heterologous expression of microbial nitroreductases for TNT degradation in transgenic animals
Heterologous expression of microbial nitroreductases for TNT degradation in transgenic animals Open
TNT (2,4,6-trinitrotoluene) from unexploded ordinances is a common environmental pollutant near munitions factories, military training sites, and areas of armed conflict. As physical and chemical approaches for TNT remediation are costly a…
View article: Selection for toxin production in spatially structured environments increases with growth rate
Selection for toxin production in spatially structured environments increases with growth rate Open
Microbes adopt diverse strategies to successfully compete with coexisting strains for space and resources. One common strategy is the production of toxic compounds to inhibit competitors, but the strength and direction of selection for thi…
View article: The strength of selection for costly toxin production increases with growth rate
The strength of selection for costly toxin production increases with growth rate Open
Microbes adopt a diversity of strategies to successfully compete with coexisting strains for space and resources. One common strategy is the production of toxic compounds to inhibit competitors, but the strength and direction of selection …
View article: High-throughput investigation of genetic design constraints in domesticated Influenza A Virus for transient gene delivery
High-throughput investigation of genetic design constraints in domesticated Influenza A Virus for transient gene delivery Open
Replication-incompetent single cycle infectious Influenza A Virus (sciIAV) has demonstrated utility as a research and vaccination platform. Protein-based therapeutics are increasingly attractive due to their high selectivity and potent eff…
View article: Corrigendum: Modeling-informed Engineered Genetic Incompatibility strategies to overcome resistance in the invasive Drosophila suzukii
Corrigendum: Modeling-informed Engineered Genetic Incompatibility strategies to overcome resistance in the invasive Drosophila suzukii Open
[This corrects the article DOI: 10.3389/finsc.2022.1063789.].
View article: Using design of experiments to guide genetic optimization of engineered metabolic pathways
Using design of experiments to guide genetic optimization of engineered metabolic pathways Open
Design of experiments (DoE) is a term used to describe the application of statistical approaches to interrogate the impact of many variables on the performance of a multivariate system. It is commonly used for process optimization in field…
View article: The Natural Products Discovery Center: Release of the First 8490 Sequenced Strains for Exploring Actinobacteria Biosynthetic Diversity
The Natural Products Discovery Center: Release of the First 8490 Sequenced Strains for Exploring Actinobacteria Biosynthetic Diversity Open
Actinobacteria, the bacterial phylum most renowned for natural product discovery, has been established as a valuable source for drug discovery and biotechnology but is underrepresented within accessible genome and strain collections. Herei…
View article: Perceptions on the genetic biocontrol of invasive carp
Perceptions on the genetic biocontrol of invasive carp Open
Common carp ( Cyprinus carpio ) is an invasive species that damages native aquatic ecosystems. Advances in genetic engineering have led to novel genetic biocontrol methods that have the potential to aid in the control of carp and other inv…
View article: Efficient gene activation in plants by the MoonTag programmable transcriptional activator
Efficient gene activation in plants by the MoonTag programmable transcriptional activator Open
CRISPR/Cas-based transcriptional activators have been developed to induce gene expression in eukaryotic and prokaryotic organisms. The main advantages of CRISPR-Cas based systems is that they can achieve high levels of transcriptional acti…
View article: Modeling-informed Engineered Genetic Incompatibility strategies to overcome resistance in the invasive Drosophila suzukii
Modeling-informed Engineered Genetic Incompatibility strategies to overcome resistance in the invasive Drosophila suzukii Open
Engineered Genetic Incompatibility (EGI) is an engineered extreme underdominance genetic system wherein hybrid animals are not viable, functioning as a synthetic speciation event. There are several strategies in which EGI could be leverage…
View article: HUGE pipeline to measure temporal genetic variation in Drosophila suzukii populations for genetic biocontrol applications
HUGE pipeline to measure temporal genetic variation in Drosophila suzukii populations for genetic biocontrol applications Open
Understanding the fine-scale genome sequence diversity that exists within natural populations is important for developing models of species migration, temporal stability, and range expansion. For invasive species, agricultural pests, and d…
View article: Development of a single culture E. coli expression system for the enzymatic synthesis of fluorinated tyrosine and its incorporation into proteins
Development of a single culture E. coli expression system for the enzymatic synthesis of fluorinated tyrosine and its incorporation into proteins Open
View article: Optimized dCas9 Programmable Transcriptional Activators for Plants
Optimized dCas9 Programmable Transcriptional Activators for Plants Open
Understanding how gene expression impacts plant development and physiology is important for rationally engineering improved crops. Programmable Transcriptional Activators (PTAs), including CRISPR-Cas activators, have traditionally relied o…
View article: Author response: Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression
Author response: Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression Open
View article: Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression
Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression Open
Engineered Genetic Incompatibility (EGI) is a method to create species-like barriers to sexual reproduction. It has applications in pest control that mimic Sterile Insect Technique when only EGI males are released. This can be facilitated …
View article: CRISPR-Cas Activators for Engineering Gene Expression in Higher Eukaryotes
CRISPR-Cas Activators for Engineering Gene Expression in Higher Eukaryotes Open
CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans. Here we review the many design variations of these versatile tools and compare their effecti…
View article: Engineering multiple species-like genetic incompatibilities in insects
Engineering multiple species-like genetic incompatibilities in insects Open
View article: Engineering multiple species-like genetic incompatibilities in insects
Engineering multiple species-like genetic incompatibilities in insects Open
Speciation constrains the flow of genetic information between populations of sexually reproducing organisms. Gaining control over mechanisms of speciation would enable new strategies to manage wild populations of disease vectors, agricultu…
View article: Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils
Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils Open
View article: Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils
Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils Open
Bacteria within the genus Streptomyces remain a major source of new natural product discovery and as soil inoculants in agriculture where they promote plant growth and protect from disease. Recently, Streptomyces spp. have been implicated …
View article: Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils
Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils Open
Bacteria within the genus Streptomyces remain a major source of new natural product discovery and as soil inoculants in agriculture where they promote plant growth and protect from disease. Recently, Streptomyces spp. have been implicated …
View article: Batch cultivation of non-transgenic males suitable for SIT programs
Batch cultivation of non-transgenic males suitable for SIT programs Open
The field performance of Sterile Insect Technique (SIT) is improved by sex-sorting and releasing only the sterile males. This can be accomplished by resource-intensive separation of males from females by morphology. Alternatively, sex-rati…
View article: The Five-Primer Challenge: An inquiry-based laboratory module for synthetic biology
The Five-Primer Challenge: An inquiry-based laboratory module for synthetic biology Open
New technologies in DNA synthesis and assembly give genetic engineers complete freedom in genetic design, where virtually any plasmid DNA sequence can be created efficiently and economically. Learning how to design, construct, and test new…
View article: Genetic manipulation of sex ratio in mammals: the Reaper comes for Mickey
Genetic manipulation of sex ratio in mammals: the Reaper comes for Mickey Open
View article: Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils
Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils Open
Background Bacteria within the genus Streptomyces remain a major source of new natural product discovery and as soil inoculants in agriculture where they promote plant growth and protect from disease. Recently, Streptomyces spp. have been …
View article: Genetic incompatibility combined with female-lethality is effective and robust in simulations of <i>Aedes aegypti</i> population control
Genetic incompatibility combined with female-lethality is effective and robust in simulations of <i>Aedes aegypti</i> population control Open
Recent reports of CRISPR/Cas9-based suppression gene drives in insects underscore the challenge of overcoming genetic resistance. Here we present results from agent-based simulation modeling of a novel Field-Amplified Male Sterility System…
View article: Simulation Modeling to Compare High-Throughput, Low-Iteration Optimization Strategies for Metabolic Engineering
Simulation Modeling to Compare High-Throughput, Low-Iteration Optimization Strategies for Metabolic Engineering Open
Increasing the final titer of a multi-gene metabolic pathway can be viewed as a multivariate optimization problem. While numerous multivariate optimization algorithms exist, few are specifically designed to accommodate the constraints pose…
View article: Engineering species-like barriers to sexual reproduction
Engineering species-like barriers to sexual reproduction Open