Michael Jahn
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View article: Targeting the translation machinery: The RNase Y specificity factor Y-complex coordinates ribosome degradation
Targeting the translation machinery: The RNase Y specificity factor Y-complex coordinates ribosome degradation Open
Limiting the synthesis and activity of ribosomes is crucial for responding to various stresses, including nutrient starvation or proteotoxic stress. In Bacillus subtilis, this can be achieved through the coordinated action of the alarmones…
View article: Novel assembly of the SF370 strain of the important human pathogen <i>Streptococcus pyogenes</i> serotype M1
Novel assembly of the SF370 strain of the important human pathogen <i>Streptococcus pyogenes</i> serotype M1 Open
The important human pathogen Streptococcus pyogenes causes a wide range of diseases. Strain SF370 was the first fully sequenced strain of S. pyogenes , providing essential insights into the molecular mechanisms of disease. We present an im…
View article: The energy metabolism of <i>Cupriavidus necator</i> in different trophic conditions
The energy metabolism of <i>Cupriavidus necator</i> in different trophic conditions Open
The “knallgas” bacterium Cupriavidus necator is attracting interest due to its extremely versatile metabolism. C. necator can use hydrogen or formic acid as an energy source, fixes CO 2 via the Calvin-Benson-Bassham (CBB) cycle, and grows …
View article: The energy metabolism of<i>Cupriavidus necator</i>in different trophic conditions
The energy metabolism of<i>Cupriavidus necator</i>in different trophic conditions Open
Summary The ‘knallgas’ bacterium Cupriavidus necator is attracting interest for biotechnological applications due to its extremely versatile metabolism. C. necator can use hydrogen or formic acid as an energy source, fixes CO 2 via the Cal…
View article: Pathway selectivity in Frizzleds is achieved by conserved micro-switches defining pathway-determining, active conformations
Pathway selectivity in Frizzleds is achieved by conserved micro-switches defining pathway-determining, active conformations Open
View article: CRISPR interference screens reveal growth–robustness tradeoffs in<i>Synechocystis</i>sp. PCC 6803 across growth conditions
CRISPR interference screens reveal growth–robustness tradeoffs in<i>Synechocystis</i>sp. PCC 6803 across growth conditions Open
Barcoded mutant libraries are a powerful tool for elucidating gene function in microbes, particularly when screened in multiple growth conditions. Here, we screened a pooled CRISPR interference library of the model cyanobacterium Synechocy…
View article: CRISPR interference screens reveal tradeoffs between growth rate and robustness in<i>Synechocystis</i>sp. PCC 6803 across trophic conditions
CRISPR interference screens reveal tradeoffs between growth rate and robustness in<i>Synechocystis</i>sp. PCC 6803 across trophic conditions Open
Barcoded mutant libraries are a powerful tool for elucidating gene function in microbes, particularly when screened in multiple growth conditions. Here, we screened a pooled CRISPR interference library of the model cyanobacterium Synechocy…
View article: Proteomic Analysis of Adenovirus 5 by UHPLC-MS/MS: Development of a Robust and Reproducible Sample Preparation Workflow
Proteomic Analysis of Adenovirus 5 by UHPLC-MS/MS: Development of a Robust and Reproducible Sample Preparation Workflow Open
Adenoviruses (AdVs) have recently become widely used therapeutic vectors for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine. AdVs are large, nonenveloped viruses with an icosahedral capsid formed from several proteins…
View article: Thermodynamic limitations of PHB production from formate and fructose in Cupriavidus necator
Thermodynamic limitations of PHB production from formate and fructose in Cupriavidus necator Open
The chemolithotroph Cupriavidus necator H16 is known as a natural producer of the bioplastic-polymer PHB, as well as for its metabolic versatility to utilize different substrates, including formate as the sole carbon and energy source. Dep…
View article: Thermodynamic limitations of metabolic strategies for PHB production from formate and fructose in <i>Cupriavidus necator</i>
Thermodynamic limitations of metabolic strategies for PHB production from formate and fructose in <i>Cupriavidus necator</i> Open
The chemolithotroph Cupriavidus necator H16 is known as a natural producer of the bioplastic-polymer PHB, as well as for its metabolic versatility to utilize different substrates, including formate as the sole carbon and energy source. Dep…
View article: Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator Open
Bacteria must balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive in their environment. Of all cellular macromolecules, the bacterial proteome is by far the most important resource an…
View article: Author response: Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
Author response: Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Bacteria must balance …
View article: Slow Protein Turnover Explains Limited Protein-Level Response to Diurnal Transcriptional Oscillations in Cyanobacteria
Slow Protein Turnover Explains Limited Protein-Level Response to Diurnal Transcriptional Oscillations in Cyanobacteria Open
Metabolically engineered cyanobacteria have the potential to mitigate anthropogenic CO 2 emissions by converting CO 2 into renewable fuels and chemicals. Yet, better understanding of metabolic regulation in cyanobacteria is required to dev…
View article: Protein allocation and utilization in the versatile chemolithoautotroph <i>Cupriavidus necator</i>
Protein allocation and utilization in the versatile chemolithoautotroph <i>Cupriavidus necator</i> Open
Summary Bacteria must balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive in their environment. Of all cellular macromolecules, the bacterial proteome is by far the most important res…
View article: New Peak Detection Performance Metrics from the MAM Consortium Interlaboratory Study
New Peak Detection Performance Metrics from the MAM Consortium Interlaboratory Study Open
The Multi-Attribute Method (MAM) Consortium was initially formed as a venue to harmonize best practices, share experiences, and generate innovative methodologies to facilitate widespread integration of the MAM platform, which is an emergin…
View article: Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes Open
View article: Pooled CRISPRi screening of the cyanobacterium<i>Synechocystis</i>sp. PCC 6803 for enhanced growth, tolerance, and chemical production
Pooled CRISPRi screening of the cyanobacterium<i>Synechocystis</i>sp. PCC 6803 for enhanced growth, tolerance, and chemical production Open
We developed an inducible CRISPRi gene repression library in the cyanobacterium Synechocystis sp. PCC 6803, where all annotated genes are targeted for repression. We used the library to estimate gene fitness in multiple conditions. The lib…
View article: Ribosome Profiling of <i>Synechocystis</i> Reveals Altered Ribosome Allocation at Carbon Starvation
Ribosome Profiling of <i>Synechocystis</i> Reveals Altered Ribosome Allocation at Carbon Starvation Open
Ribosome profiling accesses the translational step of gene expression via deep sequencing of ribosome-protected mRNA footprints. Pairing of ribosome profiling and transcriptomics data provides a translational efficiency for each gene. Here…
View article: Growth of Cyanobacteria Is Constrained by the Abundance of Light and Carbon Assimilation Proteins
Growth of Cyanobacteria Is Constrained by the Abundance of Light and Carbon Assimilation Proteins Open
View article: Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria
Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria Open
Photoautotrophic production of fuels and chemicals by cyanobacteria typically gives lower volumetric productivities and titers than heterotrophic production. Cyanobacteria cultures become light limited above an optimal cell density, so tha…
View article: Ribosome profiling in cyanobacteria
Ribosome profiling in cyanobacteria Open
Supplemental material for "Ribosome profiling of Synechocystis reveals altered ribosome allocation at carbon starvation" by Karlsen et al., 2018 (DOI: 10.1128/mSystems.00126-18).Legends.pdf contains the full legends, w…
View article: Digital mobil in Deutschlands Städten
Digital mobil in Deutschlands Städten Open
View article: Digitalisierung und Automatisierung im Öffentlichen Verkehr
Digitalisierung und Automatisierung im Öffentlichen Verkehr Open
In der Studie Digital mobil in Deutschlands Städten haben PwC und DLR untersucht, wie sehr die Digitalisierung und Automatisierung bereits heute die Mobilität in Städten beeinflussen und in welcher Weise die kommunalen Akteure (Kommunen, V…
View article: Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR Open
View article: MOESM2 of Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
MOESM2 of Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR Open
Additional file 2. This spreadsheet file contains the recommended minimal information for digital PCR experiments.
View article: MOESM4 of Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
MOESM4 of Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR Open
Additional file 4. This spreadsheet file contains the Droplet Digital PCR dataset presented in this study.
View article: MOESM1 of Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
MOESM1 of Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR Open
Additional file 1. This spreadsheet file contains statistical information on the flow cytometry data presented in this study.
View article: Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains
Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains Open
Pivotal challenges in industrial biotechnology are the identification and overcoming of cell-to-cell heterogeneity in microbial processes. While the development of subpopulations of isogenic cells in bioprocesses is well described (intra-p…
View article: Characterization of population heterogeneity in a model biotechnological process using Pseudomonas putida
Characterization of population heterogeneity in a model biotechnological process using Pseudomonas putida Open
Biotechnological processes are distinguished from classical chemistry by employing bio-molecules or whole cells as the catalytic element, providing unique reaction mechanisms with unsurpassed specificity. Whole cells are the most versatile…
View article: R as an Environment for Reproducible Analysis of DNA Amplification Experiments
R as an Environment for Reproducible Analysis of DNA Amplification Experiments Open
There is an ever-increasing number of applications, which use quantitative PCR (qPCR) or digital PCR (dPCR) to elicit fundamentals of biological processes.Moreover, quantitative isothermal amplification (qIA) methods have become more promi…