Michael Kickstein
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View article: Hydroxamate Assays for High‐Throughput Screening of Transketolase Libraries Against Arylated Substrates
Hydroxamate Assays for High‐Throughput Screening of Transketolase Libraries Against Arylated Substrates Open
We recently reported that the transketolase from Geobacillus stearothermophilus (TK gst ) upon acyl transfer to nitrosoarenes generates N ‐aryl hydroxamic acids (HA). The latter are metal chelating compounds that in the presence of Fe(III)…
View article: An α2,3‐Sialyltransferase from <i>Photobacterium phosphoreum</i> with Broad Substrate Scope: Controlling Hydrolytic Activity by Directed Evolution
An α2,3‐Sialyltransferase from <i>Photobacterium phosphoreum</i> with Broad Substrate Scope: Controlling Hydrolytic Activity by Directed Evolution Open
Defined sialoglycoconjugates are important molecular probes for studying the role of sialylated glycans in biological systems. We show that the α2,3‐sialyltransferase from Photobacterium phosphoreum JT‐ISH‐467 (2,3SiaT pph ) tolerates a ve…
View article: Minimalist Protein Engineering of an Aldolase Provokes Unprecedented Substrate Promiscuity
Minimalist Protein Engineering of an Aldolase Provokes Unprecedented Substrate Promiscuity Open
Application of aldolases for the asymmetric synthesis of multifunctional chiral products is hampered by their reputed strict nucleophile (=aldol donor) specificity owing to a mechanistic requirement for creating a carbanion nucleophile in …