Explanipedia

L-2-hydroxyglutarate regulates centromere and heterochromatin conformation in the male germline Open

Nina Mayorek, Miriam Schlossberg, Yousef Mansour, Nir Pillar, Ilan Stein , et al. · 2025

Germ cell differentiation in the male testis involves extensive phenotypic, transcriptional, and epigenetic modifications, which are essential for producing functional spermatozoa. Among all organs, the testis exhibits the highest baseline…

Epigenetic aging in oocytes Open

Peera Wasserzug‐Pash, Michael Klutstein · 2023

Overexpressed kinetochore genes are used by cancer cells as genome destabilizers and transformation catalysts Open

Reem Kamal Aldwaik, Denen Shian, Roshina Thapa, Swetha Vasudevan, Mimi Abo-Ayoub Ashqar , et al. · 2023

Cancer cells have an altered transcriptome, which contributes to their abnormal behavior. Many tumors have high levels of kinetochore genes, which play important roles in genome stability. This overexpression could be utilized to destabili…

Oocyte Aging: A Multifactorial Phenomenon in A Unique Cell Open

Paweł Kordowitzki, Szymon Graczyk, Amin Haghani, Michael Klutstein · 2023

The oocyte is considered to be the largest cell in mammalian species. Women hoping to become pregnant face a ticking biological clock. This is becoming increasingly challenging as an increase in life expectancy is accompanied by the tenden…

Pre-pubertal oocytes harbor altered histone modifications and chromatin configuration Open

Pe’era Wasserzug Pash, Gilad Karavani, Eli Reich, Lital Zecharyahu, Zehava Kay , et al. · 2023

Pre-pubertal oocytes are still dormant. They are arrested in a GV state and do not undergo meiotic divisions naturally. A multitude of molecular pathways are changed and triggered upon initiation of puberty. It is not yet clear which epige…

Editorial: Germ cell development and reproductive aging Open

Miguel A. Brieño‐Enríquez, Francesca E. Duncan, Arjumand Ghazi, Michael Klutstein, Vittorio Sebastiano , et al. · 2022

EDITORIAL article Front. Cell Dev. Biol., 12 October 2022Sec. Cellular Biochemistry Volume 10 - 2022 | https://doi.org/10.3389/fcell.2022.1051539

2-hydroxyglutarate controls centromere and heterochromatin conformation and function in the male germline Open

Nina Mayorek, Miriam Schlossberg, Yousef Mansour, Nir Pillar, Ilan Stein , et al. · 2022

2-hydroxyglutarate (2HG) is recognized as an epigenetic regulator in cancer and some transient biological processes. Of all organs, the testis harbors the highest baseline physiological levels of 2HG, yet it’s putative functions in germ ce…

Lef1 ablation alleviates cartilage mineralization following posttraumatic osteoarthritis induction Open

Jinan Elayyan, Idan Carmon, Lital Zecharyahu, George Batshon, Yonathan H. Maatuf , et al. · 2022

Significance Cartilage mineralization is imperative in various processes such as skeletal growth and fracture repair. However, this process may also be pathological, as in the case of the degenerative joint disease, osteoarthritis (OA). Us…

Loss of heterochromatin and retrotransposon silencing as determinants in oocyte aging Open

Peera Wasserzug‐Pash, Rachel Rothman, Eli Reich, Lital Zecharyahu, Oshrat Schonberger , et al. · 2022

Mammalian oocyte quality reduces with age. We show that prior to the occurrence of significant aneuploidy (9M in mouse), heterochromatin histone marks are lost, and oocyte maturation is impaired. This loss occurs in both constitutive and f…

Endurance of extremely prolonged nutrient prevention across kingdoms of life Open

Ronen Hazan, Miriam Schoemann, Michael Klutstein · 2021

Age-Dependent in vitro Maturation Efficacy of Human Oocytes – Is There an Optimal Age? Open

Gilad Karavani, Peera Wasserzug‐Pash, Talya Mordechai-Daniel, Dvora Bauman, Michael Klutstein , et al. · 2021

In vitro maturation of oocytes from antral follicles seen during tissue harvesting is a fertility preservation technique with potential advantages over ovarian tissue cryopreservation (OTC), as mature frozen and later thawed oocyte used fo…

supplementary material for Thapa et al. 2021 v2 Open

Michael Klutstein · 2021

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supplementary material for Thapa et al. 2021 Open

Michael Klutstein · 2021

supplementary material for Thapa et al. 2021

A Mouse Model for Studying the Development of Apical Periodontitis with Age Open

Elisheva Goldman, Eli Reich, Bar Roshihotzki, Maya Saketkhou, Sharon Wald , et al. · 2021

Older age is associated with reduced immune function. Our aim was to study how age affects the development of apical periodontitis (AP). AP was induced in two age groups of mice (young vs. adult). Histological samples were stained by Hemat…

Centromeres are dismantled by foundational meiotic proteins Spo11 and Rec8 Open

Haitong Hou, Eftychia Kyriacou, Rahul Thadani, Michael Klutstein, Joseph H. Chapman , et al. · 2021

Loss of heterochromatin and retrotransposon silencing constitute an early phase in oocyte aging Open

Peera Wasserzug‐Pash, Rachel Rothman, Eli Reich, Oshrat Schonberger, Yifat Weiss , et al. · 2020

Mammalian oocyte quality reduces with female age. A well-studied aspect of this deterioration is an age-associated rise in oocyte aneuploidy. We show that prior to the occurrence of significant aneuploidy (at the age of 9 months in mouse f…

Colon Cancer-Associated Fusobacterium nucleatum May Originate From the Oral Cavity and Reach Colon Tumors via the Circulatory System Open

Jawad Abed, Naseem Maalouf, Abigail L. Manson, Ashlee M. Earl, Lishay Parhi , et al. · 2020

Fusobacterium nucleatum is a common oral bacterium that is enriched in colorectal adenomas and adenocarcinomas (CRC). In humans, high fusobacterial CRC abundance is associated with chemoresistance and poor prognosis. In animal model…

supplementary material for Thapa et al. 2020 Open

Michael Klutstein · 2020

Supplementary material related to Thapa et al. 2020- publication by the Klutstein and Kravchenko-Balasha labs

supplementary material for Thapa et al. 2020 Open

Michael Klutstein · 2020

Supplementary material related to Thapa et al. 2020- publication by the Klutstein and Kravchenko-Balasha labs

Isolation and Characterization of Live Yeast Cells from Ancient Clay Vessels Open

Tzemach Aouizerat, Aren M. Maeir, Yitzhak Paz, Yuval Gadot, Amir Szitenberg , et al. · 2020

Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied us…

Meiosis and Kinetochore genes are used by cancer cells as genome destabilizers and transformation catalysts Open

Roshina Thapa, Swetha Vasudevan, Mimi Abo-Ayoub Ashqar, Eli Reich, Nataly Kravchenko‐Balasha , et al. · 2019

Cancer cells have an altered transcriptome which contributes to their altered behaviors compared to normal cells. Indeed, many tumors express high levels of genes participating in meiosis or kinetochore biology, but the role of this high e…

Eukaryotic Adaptation to Years-Long Starvation Resembles that of Bacteria Open

Tzemach Aouizerat, Daniel Gelman, Amir Szitenberg, Itay Gutman, Shunit Glazer , et al. · 2019

Isolation and Characterization of Live Yeast Cells from Ancient Vessels as a Tool in Bio-Archaeology Open

Tzemach Aouizerat, Itai Gutman, Yitzhak Paz, Aren M. Maeir, Yuval Gadot , et al. · 2019

So far, most of the study of ancient organisms has been based mainly on the analysis of ancient DNA. Here we show that it is possible to isolate and study microorganisms—yeast in this case—from ancient pottery vessels used for fermentation…

Evolthon: A community endeavor to evolve lab evolution Open

Sivan Kaminski Strauss, Dvir Schirman, Ghil Jona, Aaron N. Brooks, Aditya M. Kunjapur , et al. · 2019

In experimental evolution, scientists evolve organisms in the lab, typically by challenging them to new environmental conditions. How best to evolve a desired trait? Should the challenge be applied abruptly, gradually, periodically, sporad…

Isolation and characterization of live yeast cells from ancient vessels as a tool in bio-archeology Open

Tzemach Aouizerat, Itai Gutman, Yitzhak Paz, Aren M. Maeir, Yuval Gadot , et al. · 2019

Ancient fermented food has been studied based on recipes, residue analysis and ancient-DNA techniques and reconstructed using modern domesticated yeast. Here, we present a novel approach. We hypothesize that enriched yeast populations in f…

Contribution of epigenetic mechanisms to variation in cancer risk among tissues Open

Michael Klutstein, Joshua Moss, Tommy Kaplan, Howard Cedar · 2017

Significance It is well-known that the risk of cancer varies widely between different tissues of the body with some cell types (e.g., colon, breast) being prone to cancer, whereas others (e.g., brain, bone) only rarely develop tumors, but …

The telomere bouquet regulates meiotic centromere assembly Open

Michael Klutstein, Alex Fennell, Alfonso Fernández-Álvarez, Julia Promisel Cooper · 2015

Michael Klutstein YOU? Author Swipe