Michael Krieg
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View article: Seeing in the Dark: Intelligent Fourier Light Field Imaging for Bioluminescence Microscopy
Seeing in the Dark: Intelligent Fourier Light Field Imaging for Bioluminescence Microscopy Open
Bioluminescence microscopy offers a uniquely non-invasive window into cellular dynamics, yet its use has traditionally been limited by the intrinsically low brightness of luciferases. The poor photon budget forces long exposures, preventin…
View article: The mechanobiology of biomolecular condensates
The mechanobiology of biomolecular condensates Open
The central goal of mechanobiology is to understand how the mechanical forces and material properties of organelles, cells, and tissues influence biological processes and functions. Since the first description of biomolecular condensates, …
View article: Periodic Obstacles Regulate Membrane Tension Propagation to Enable Localized Mechanotransduction
Periodic Obstacles Regulate Membrane Tension Propagation to Enable Localized Mechanotransduction Open
Forces applied to cellular membranes lead to transient membrane tension gradients. The manner in which membrane tension propagates away from the stimulus into the membrane reservoir has emerged as a key property in cellular adaptation, mec…
View article: Sensory experience controls dendritic structure and behavior by distinct pathways involving degenerins
Sensory experience controls dendritic structure and behavior by distinct pathways involving degenerins Open
Dendrites are crucial for receiving information into neurons. Sensory experience affects the structure of these tree-like neurites, which, it is assumed, modifies neuronal function, yet the evidence is scarce, and the mechanisms are unknow…
View article: Measuring age-dependent viscoelasticity of organelles, cells and organisms with time-shared optical tweezer microrheology
Measuring age-dependent viscoelasticity of organelles, cells and organisms with time-shared optical tweezer microrheology Open
Quantifying the mechanical response of the biological milieu (such as the cell’s interior) and complex fluids (such as biomolecular condensates) would enable a better understanding of cellular differentiation and aging and accelerate drug …
View article: Mechanical load conditions the spectrin network to ‘runon’ proteolysis and promotes early onset neurodegeneration
Mechanical load conditions the spectrin network to ‘runon’ proteolysis and promotes early onset neurodegeneration Open
The correct homeostasis of the neuronal cytoskeleton and its dynamics is important for health and disease. Forces constantly act on the neurons in our body, leading to subtle axonal deformations and length changes. The spectrin cytoskeleto…
View article: Automated dual olfactory device for studying head/tail chemosensation in <i>Caenorhabditis elegans</i>
Automated dual olfactory device for studying head/tail chemosensation in <i>Caenorhabditis elegans</i> Open
The correct interpretation of threat and reward is important for animal survival. Often, the decisions underlying these behavioral programs are mediated by volatile compounds in the animal's environment, which they detect and discriminate …
View article: Bioluminescence as a functional tool for visualizing and controlling neuronal activity in vivo
Bioluminescence as a functional tool for visualizing and controlling neuronal activity in vivo Open
The use of bioluminescence as a reporter for physiology in neuroscience is as old as the discovery of the calcium-dependent photon emission of aequorin. Over the years, luciferases have been largely replaced by fluorescent reporters, but r…
View article: Measuring age-dependent viscoelastic properties of organelles, cells and organisms via Time-Shared Optical Tweezer Microrheology
Measuring age-dependent viscoelastic properties of organelles, cells and organisms via Time-Shared Optical Tweezer Microrheology Open
Recording the mechanical response of biological samples, the cell’s interior and complex fluids in general, would enable deeper understanding of cellular differentiation, ageing and drug discovery. Here, we present a time-shared optical tw…
View article: Integration of spatially opposing cues by a single interneuron guides decision-making in C. elegans
Integration of spatially opposing cues by a single interneuron guides decision-making in C. elegans Open
The capacity of animals to respond to hazardous stimuli in their surroundings is crucial for their survival. In mammals, complex evaluations of the environment require large numbers and different subtypes of neurons. The nematode C. elegan…
View article: Ca2+ imaging data for: A rigidity transition of MEC-2/Stomatin condensates controls neuronal mechanotransduction during touch sensing
Ca2+ imaging data for: A rigidity transition of MEC-2/Stomatin condensates controls neuronal mechanotransduction during touch sensing Open
Calcium imaging data from Sanfeliu et al, NCB, 2023. Project contains fluorescence data to characterize the activity of the touch receptor neurons (TRNs) - which are mechanically activated upon touch – in the model organism Caenorhabditis …
View article: Ca2+ imaging data for: A rigidity transition of MEC-2/Stomatin condensates controls neuronal mechanotransduction during touch sensing
Ca2+ imaging data for: A rigidity transition of MEC-2/Stomatin condensates controls neuronal mechanotransduction during touch sensing Open
Calcium imaging data from Sanfeliu et al, NCB, 2023. Project contains fluorescence data to characterize the activity of the touch receptor neurons (TRNs) - which are mechanically activated upon touch – in the model organism Caenorhabditis …
View article: FLInt: single shot safe harbor transgene integration via <i>F</i>luorescent <i>L</i>andmark <i>Int</i>erference
FLInt: single shot safe harbor transgene integration via <i>F</i>luorescent <i>L</i>andmark <i>Int</i>erference Open
The stable incorporation of transgenes and recombinant DNA material into the host genome is a bottleneck in many bioengineering applications. Due to the low efficiency, identifying the transgenic animals is often a needle in the haystack. …
View article: Integration of spatially opposing cues by a single interneuron guides decision making in<i>C. elegans</i>
Integration of spatially opposing cues by a single interneuron guides decision making in<i>C. elegans</i> Open
The capacity of animals to integrate and respond to multiple hazardous stimuli in the surroundings is crucial for their survival. In mammals, complex evaluations of the environment require large numbers and different subtypes of neurons. T…
View article: FLInt: Single Shot Safe Harbor Transgene Integration via<i>F</i>luorescent<i>L</i>andmark<i>Int</i>erference
FLInt: Single Shot Safe Harbor Transgene Integration via<i>F</i>luorescent<i>L</i>andmark<i>Int</i>erference Open
The stable incorporation of transgenes and recombinant DNA material into the host genome is a bottleneck in many bioengineering applications. Due to the low efficiency, identifying the transgenic animals is often a needle in the haystack. …
View article: Volumetric imaging of cellular dynamics with deep learning enhanced bioluminescence microscopy
Volumetric imaging of cellular dynamics with deep learning enhanced bioluminescence microscopy Open
The low photon emission of known luciferases, currently limit their widespread use as contrast agents in live cell microscopy because they demand long exposure times that are prohibitive for imaging fast biological dynamics. To increase th…
View article: Volumetric imaging of cellular dynamics with deep learning enhanced bioluminescence microscopy
Volumetric imaging of cellular dynamics with deep learning enhanced bioluminescence microscopy Open
The low photon emission of known luciferases, currently limit their widespread use as contrast agents in live cell microscopy because they demand long exposure times that are prohibitive for imaging fast biological dynamics. To increase th…
View article: Exploring cell and tissue mechanics with optical tweezers
Exploring cell and tissue mechanics with optical tweezers Open
Cellular and tissue biosystems emerge from the assembly of their constituent molecules and obtain a set of specific material properties. To measure these properties and understand how they influence cellular function is a central goal of m…
View article: A rigidity phase transition of Stomatin condensates governs a switch from transport to mechanotransduction
A rigidity phase transition of Stomatin condensates governs a switch from transport to mechanotransduction Open
A large body of work suggests that biomolecular condensates ensuing from liquid-liquid phase separation mature into various material states. How this aging process is controlled and if the naive and mature phases can have differential func…
View article: Volumetric bioluminescence imaging of cellular dynamics with deep learning based light-field reconstruction
Volumetric bioluminescence imaging of cellular dynamics with deep learning based light-field reconstruction Open
The application of genetically encoded fluorophores for microscopy has afforded one of the biggest revolutions in the biosciences. Bioluminescence microscopy is an appealing alternative to fluorescence microscopy, because it does not depen…
View article: Axonal plasticity in response to active forces generated through magnetic nanopulling
Axonal plasticity in response to active forces generated through magnetic nanopulling Open
Summary Mechanical force is crucial in guiding axon outgrowth, before and after synapse formation. This process is referred to as “stretch-growth”. However, how neurons transduce mechanical inputs into signaling pathways remains poorly und…