Michael Lammers
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View article: E3 ubiquitin ligase SKP2 limits autophagy during <i>S. aureus</i> infection
E3 ubiquitin ligase SKP2 limits autophagy during <i>S. aureus</i> infection Open
Ubiquitination is a posttranslational modification that affects protein function, stability, and localization and is thereby balancing protein homeostasis. During infection, ubiquitination is crucial in regulating host cell signaling pathw…
View article: Protein Target Highlights in <scp>CASP16</scp> : Insights From the Structure Providers
Protein Target Highlights in <span>CASP16</span> : Insights From the Structure Providers Open
This article presents an in‐depth analysis of selected CASP16 targets, with a focus on their biological and functional significance. The authors highlight the most relevant features of the target proteins and discuss how well these were re…
View article: Insights into a water-mediated catalytic triad architecture in CE20 carbohydrate esterases
Insights into a water-mediated catalytic triad architecture in CE20 carbohydrate esterases Open
View article: Reprogramming of bacterial virulence by lysine acetylation
Reprogramming of bacterial virulence by lysine acetylation Open
Gram-negative bacteria use a plethora of virulence factors to infect eukaryotic cells. CE-clan protease-related virulence factors were reported to act as deubiquitinases/ubiquitin-like specific proteases. Some have an additional acetyltran…
View article: Identification and Protein Engineering of Galactosidases for the Conversion of Blood Type B to Blood Type O
Identification and Protein Engineering of Galactosidases for the Conversion of Blood Type B to Blood Type O Open
The supply of blood products such as red blood cells poses a challenge due to rising demand and declining donor numbers. Careful matching of blood products of different types is required. Only type O of the blood types A, B, AB and O can b…
View article: Staphylococcal SplA and SplB serine proteases target ubiquitin(-like) specific proteases
Staphylococcal SplA and SplB serine proteases target ubiquitin(-like) specific proteases Open
Staphylococcus aureus is a Gram-positive opportunistic pathogen that has colonized nearly 30% of the human population and can cause life-threatening infections. S. aureus exports a variety of virulence factors, such as a novel set of extra…
View article: Biochemical analyses of cystatin-C dimers and cathepsin-B reveals a trypsin-driven feedback mechanism in acute pancreatitis
Biochemical analyses of cystatin-C dimers and cathepsin-B reveals a trypsin-driven feedback mechanism in acute pancreatitis Open
View article: Distribution and diversity of classical deacylases in bacteria
Distribution and diversity of classical deacylases in bacteria Open
View article: Acetyl-CoA synthetase activity is enzymatically regulated by lysine acetylation using acetyl-CoA or acetyl-phosphate as donor molecule
Acetyl-CoA synthetase activity is enzymatically regulated by lysine acetylation using acetyl-CoA or acetyl-phosphate as donor molecule Open
The AMP-forming acetyl-CoA synthetase is regulated by lysine acetylation both in bacteria and eukaryotes. However, the underlying mechanism is poorly understood. The Bacillus subtilis acetyltransferase AcuA and the AMP-forming acetyl-CoA s…
View article: A Novel C3/C4-Fused Indole Scaffold through Acid-Catalyzed Cascade Reaction
A Novel C3/C4-Fused Indole Scaffold through Acid-Catalyzed Cascade Reaction Open
3,4-bridged indoles are underrepresented among the vast number of indoles described in the literature. Attempts to access 3,4-macrocyclized indoles led to the unexpected formation of a novel tetracyclic indole through intramolecular acid-c…
View article: Reduced interleukin-18 secretion by human monocytic cells in response to infections with hyper-virulent Streptococcus pyogenes
Reduced interleukin-18 secretion by human monocytic cells in response to infections with hyper-virulent Streptococcus pyogenes Open
View article: Bacteria employ lysine acetylation of transcriptional regulators to adapt gene expression to cellular metabolism
Bacteria employ lysine acetylation of transcriptional regulators to adapt gene expression to cellular metabolism Open
View article: Marine <i>Bacteroidetes</i> enzymatically digest xylans from terrestrial plants
Marine <i>Bacteroidetes</i> enzymatically digest xylans from terrestrial plants Open
Marine Bacteroidetes that degrade polysaccharides contribute to carbon cycling in the ocean. Organic matter, including glycans from terrestrial plants, might enter the oceans through rivers. Whether marine bacteria degrade structurally rel…
View article: Structural Insights into (Tere)phthalate-Ester Hydrolysis by a Carboxylesterase and Its Role in Promoting PET Depolymerization
Structural Insights into (Tere)phthalate-Ester Hydrolysis by a Carboxylesterase and Its Role in Promoting PET Depolymerization Open
TfCa, a promiscuous carboxylesterase from Thermobifida fusca, was found to hydrolyze polyethylene terephthalate (PET) degradation intermediates such as bis(2-hydroxyethyl) terephthalate (BHET) and mono-(2-hydroxyethyl)-terephthalate…
View article: Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase
Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase Open
Thermophilic polyester hydrolases (PES-H) have recently enabled biocatalytic recycling of the mass-produced synthetic polyester polyethylene terephthalate (PET), which has found widespread use in the packaging and textile industries. The g…
View article: Structural Basis of the Pancreatitis-Associated Autoproteolytic Failsafe Mechanism in Human Anionic Trypsin
Structural Basis of the Pancreatitis-Associated Autoproteolytic Failsafe Mechanism in Human Anionic Trypsin Open
We provide a structure of TRY2, which is the predominant trypsin isoform in chronic pancreatitis and pancreatic cancer. A proposed autoinhibition mode was confirmed and the structural basis of the autoproteolytic failsafe mechanism elucida…
View article: Structural and Biophysical Insights into SPINK1 Bound to Human Cationic Trypsin
Structural and Biophysical Insights into SPINK1 Bound to Human Cationic Trypsin Open
(1) The serine protease inhibitor Kazal type 1 (SPINK1) inhibits trypsin activity in zymogen granules of pancreatic acinar cells. Several mutations in the SPINK1 gene are associated with acute recurrent pancreatitis (ARP) and chronic pancr…
View article: Diversification by CofC and Control by CofD Govern Biosynthesis and Evolution of Coenzyme F <sub>420</sub> and Its Derivative 3PG-F <sub>420</sub>
Diversification by CofC and Control by CofD Govern Biosynthesis and Evolution of Coenzyme F <sub>420</sub> and Its Derivative 3PG-F <sub>420</sub> Open
The microbial cofactor F 420 is crucial for processes like methanogenesis, antibiotics biosynthesis, drug resistance, and biocatalysis. Recently, a novel derivative of F 420 (3PG-F 420 ) was discovered, enabling the production and use of F…
View article: Lysine acetylation regulates the interaction between proteins and membranes
Lysine acetylation regulates the interaction between proteins and membranes Open
View article: Assays to Study Enzymatic and Non‐Enzymatic Protein Lysine Acetylation <i>In Vitro</i>
Assays to Study Enzymatic and Non‐Enzymatic Protein Lysine Acetylation <i>In Vitro</i> Open
Proteins can be lysine‐acetylated both enzymatically, by lysine acetyltransferases (KATs), and non‐enzymatically, by acetyl‐CoA and/or acetyl‐phosphate. Such modification can be reversed by lysine deacetylases classified as NAD + ‐dependen…
View article: Post-translational Lysine Ac(et)ylation in Bacteria: A Biochemical, Structural, and Synthetic Biological Perspective
Post-translational Lysine Ac(et)ylation in Bacteria: A Biochemical, Structural, and Synthetic Biological Perspective Open
Ac(et)ylation is a post-translational modification present in all domains of life. First identified in mammals in histones to regulate RNA synthesis, today it is known that is regulates fundamental cellular processes also in bacteria: tran…
View article: Post-translational lysine ac(et)ylation in health, ageing and disease
Post-translational lysine ac(et)ylation in health, ageing and disease Open
The acetylation/acylation (ac(et)ylation) of lysine side chains is a dynamic post-translational modification (PTM) regulating fundamental cellular processes with implications on the organisms’ ageing process: metabolism, transcription, tra…
View article: Diversification by CofC and control by CofD govern biosynthesis and evolution of coenzyme F<sub>420</sub> and its derivative 3PG-F<sub>420</sub>
Diversification by CofC and control by CofD govern biosynthesis and evolution of coenzyme F<sub>420</sub> and its derivative 3PG-F<sub>420</sub> Open
Coenzyme F 420 is a microbial redox cofactor that is increasingly used for biocatalytic applications. Recently, diversified biosynthetic routes to F 420 and the discovery of a derivative, 3PG-F 420 , were reported. 3PG-F 420 is formed via …
View article: Correction to: The development of genome editing tools as powerful techniques with versatile applications in biotechnology and medicine: CRISPR/Cas9, ZnF- and TALE-nucleases, RNA interference and Cre/loxP
Correction to: The development of genome editing tools as powerful techniques with versatile applications in biotechnology and medicine: CRISPR/Cas9, ZnF- and TALE-nucleases, RNA interference and Cre/loxP Open
We regret the incorrect designation of the site attacking the 3′-phosphotyrosine as 3′-hydroxyl group in the process following the formation of the Holliday junction intermediate to complete Cre/ loxP mediated recombination of homologous D…
View article: The development of genome editing tools as powerful techniques with versatile applications in biotechnology and medicine: CRISPR/Cas9, ZnF and TALE nucleases, RNA interference, and Cre/loxP
The development of genome editing tools as powerful techniques with versatile applications in biotechnology and medicine: CRISPR/Cas9, ZnF and TALE nucleases, RNA interference, and Cre/loxP Open
The huge progress in whole genome sequencing (genomic revolution) methods including next generation sequencing (NGS) techniques allows one to obtain data on genome sequences of all organisms, ranging from bacteria to plants to mammals, wit…
View article: Discovery and Design of Family VIII Carboxylesterases as Highly Efficient Acyltransferases
Discovery and Design of Family VIII Carboxylesterases as Highly Efficient Acyltransferases Open
Promiscuous acyltransferase activity is the ability of certain hydrolases to preferentially catalyze acyl transfer over hydrolysis, even in bulk water. However, poor enantioselectivity, low transfer efficiency, significant product hydrolys…
View article: Entdeckung und Design promiskuitiver Acyltransferase‐Aktivität in Carboxylesterasen der Familie VIII
Entdeckung und Design promiskuitiver Acyltransferase‐Aktivität in Carboxylesterasen der Familie VIII Open
Promiskuitive Acyltransferase‐Aktivität ist die Eigenschaft mancher Hydrolasen, in wässriger Lösung Acyltransfer statt Hydrolyse zu katalysieren. Niedrige Enantioselektivität bzw. Effizienz, signifikante Produkthydrolyse und das begrenzte …
View article: An evolutionary approach to systematic discovery of novel deubiquitinases, applied to <i>Legionella</i>
An evolutionary approach to systematic discovery of novel deubiquitinases, applied to <i>Legionella</i> Open
Deubiquitinating enzymes (DUBs) are important regulators of the posttranslational protein ubiquitination system. Mammalian genomes encode about 100 different DUBs, which can be grouped into seven different classes. Members of other DUB cla…
View article: An evolutionary approach to systematic discovery of novel deubiquitinases, applied to Legionella
An evolutionary approach to systematic discovery of novel deubiquitinases, applied to Legionella Open
Deubiquitinating enzymes (DUBs) are important regulators of the posttranslational protein ubiquitination system. Mammalian genomes encode about hundred different DUBs, which can be grouped into seven different classes. Members of other DUB…
View article: Sequence‐Based Prediction of Promiscuous Acyltransferase Activity in Hydrolases
Sequence‐Based Prediction of Promiscuous Acyltransferase Activity in Hydrolases Open
Certain hydrolases preferentially catalyze acyl transfer over hydrolysis in an aqueous environment. However, the molecular and structural reasons for this phenomenon are still unclear. Herein, we provide evidence that acyltransferase activ…