Michael Lebens
YOU?
Author Swipe
View article: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection
Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection Open
Influenza remains a persistent global health challenge, largely due to the virus’ continuous antigenic drift and shift, which impede the development of a universal vaccine. To address this, the identification of broadly neutralizing antibo…
View article: Cholera intoxication of human enteroids reveals interplay between decoy and functional glycoconjugate ligands
Cholera intoxication of human enteroids reveals interplay between decoy and functional glycoconjugate ligands Open
Prior research on cholera toxin (CT) binding and intoxication has relied on human colonic cancer derived epithelial cells. While these transformed cell lines have been beneficial, they neither derive from small intestine where intoxication…
View article: A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine
A thermostable, dry formulation inactivated Hikojima whole cell/cholera toxin B subunit oral cholera vaccine Open
The feared diarrheal disease cholera remains an important global health problem. Use of oral cholera vaccine (OCV) from a global stockpile against both epidemic and endemic cholera is a cornerstone in the World Health Organisations (WHOs) …
View article: Bacterial travellers’ diarrhoea: A narrative review of literature published over the past 10 years
Bacterial travellers’ diarrhoea: A narrative review of literature published over the past 10 years Open
Travellers' diarrhoea (TD) is the most frequent illness experienced by international travellers to lower-income countries with bacterial agents considered to account for 80-90% of cases. In this review, we summarise evidence published on b…
View article: Characterization of the ganglioside recognition profile of <i>Escherichia coli</i> heat-labile enterotoxin LT-IIc
Characterization of the ganglioside recognition profile of <i>Escherichia coli</i> heat-labile enterotoxin LT-IIc Open
The heat-labile enterotoxins of Escherichia coli and cholera toxin of Vibrio cholerae are related in structure and function. Each of these oligomeric toxins is comprised of one A polypeptide and five B polypeptides. The B-subunits bind to …
View article: Immune responses against oxidized LDL as possible targets for prevention of atherosclerosis in systemic lupus erythematosus
Immune responses against oxidized LDL as possible targets for prevention of atherosclerosis in systemic lupus erythematosus Open
Patients suffering from systemic lupus erythematosus (SLE) are at increased risk of developing cardiovascular disease (CVD) and traditional therapies including statins provide insufficient protection. Impaired removal of apoptotic material…
View article: Development of Hillchol®, a low-cost inactivated single strain Hikojima oral cholera vaccine
Development of Hillchol®, a low-cost inactivated single strain Hikojima oral cholera vaccine Open
Cholera remains an important global health problem with up to 4 million cases and 140,000 deaths annually. Oral cholera vaccines (OCVs) are now a cornerstone of the WHOs "Ending Cholera - A Global Roadmap to 2030" global program for the ev…
View article: Serotype has a significant impact on the virulence of 7th pandemic<i>Vibrio cholerae</i>O1
Serotype has a significant impact on the virulence of 7th pandemic<i>Vibrio cholerae</i>O1 Open
Of over 200 different identified Vibrio cholerae serogroups only the O1 serogroup is consistently associated with endemic and epidemic cholera disease. The O1 serogroup has two serologically distinguishable variants, the Ogawa and Inaba se…
View article: A New Strategy Toward B Cell-Based Cancer Vaccines by Active Immunization With Mimotopes of Immune Checkpoint Inhibitors
A New Strategy Toward B Cell-Based Cancer Vaccines by Active Immunization With Mimotopes of Immune Checkpoint Inhibitors Open
Therapeutic monoclonal antibodies (mAbs), targeting tumor antigens, or immune checkpoints, have demonstrated a remarkable anti-tumor effect against various malignancies. However, high costs for mono- or combination therapies, associated wi…
View article: Alpha-galactosylceramide enhances mucosal immunity to oral whole-cell cholera vaccines
Alpha-galactosylceramide enhances mucosal immunity to oral whole-cell cholera vaccines Open
Cholera is a severe diarrheal disease caused by the bacterium Vibrio cholerae (V. cholerae) that results in 3-4 million cases globally with 100,000-150,000 deaths reported annually. Mostly confined to developing nations, current strategies…
View article: Requirement for Cyclic AMP/Protein Kinase A-Dependent Canonical NFκB Signaling in the Adjuvant Action of Cholera Toxin and Its Non-toxic Derivative mmCT
Requirement for Cyclic AMP/Protein Kinase A-Dependent Canonical NFκB Signaling in the Adjuvant Action of Cholera Toxin and Its Non-toxic Derivative mmCT Open
Cholera toxin (CT) is widely used as an effective adjuvant in experimental immunology for inducing mucosal immune responses; yet its mechanisms of adjuvant action remain incompletely defined. Here, we demonstrate that mice lacking NFκB, co…
View article: GM1 ganglioside-independent intoxication by Cholera toxin
GM1 ganglioside-independent intoxication by Cholera toxin Open
Cholera toxin (CT) enters and intoxicates host cells after binding cell surface receptors via its B subunit (CTB). We have recently shown that in addition to the previously described binding partner ganglioside GM1, CTB binds to fucosylate…
View article: A Novel Nonantibiotic, <i>lgt</i> -Based Selection System for Stable Maintenance of Expression Vectors in Escherichia coli and Vibrio cholerae
A Novel Nonantibiotic, <i>lgt</i> -Based Selection System for Stable Maintenance of Expression Vectors in Escherichia coli and Vibrio cholerae Open
Antibiotic selection for the maintenance of expression plasmids is discouraged in the production of recombinant proteins for pharmaceutical or other human uses due to the risks of antibiotic residue contamination of the final products and …
View article: Cholera toxin binds to LewisX and fucosylated glycoproteins play a functional role in human intestinal cell intoxication
Cholera toxin binds to LewisX and fucosylated glycoproteins play a functional role in human intestinal cell intoxication Open
Cholera toxin (CT) enters and intoxicates host cells after binding cell surface receptors via its B subunit (CTB). We have recently shown that in addition to the previously described binding partner ganglioside GM1, CTB binds to fucosylate…
View article: Retrospective Analysis of Serotype Switching of Vibrio cholerae O1 in a Cholera Endemic Region Shows It Is a Non-random Process
Retrospective Analysis of Serotype Switching of Vibrio cholerae O1 in a Cholera Endemic Region Shows It Is a Non-random Process Open
Genomic data generated from clinical Vibrio cholerae O1 isolates collected over a five year period in an area of Kolkata, India with seasonal cholera outbreaks allowed a detailed genetic analysis of serotype switching that occurred from Og…
View article: Biochemical and structural characterization of the novel sialic acid-binding site of <i>Escherichia coli</i> heat-labile enterotoxin LT-IIb
Biochemical and structural characterization of the novel sialic acid-binding site of <i>Escherichia coli</i> heat-labile enterotoxin LT-IIb Open
The structurally related AB5-type heat-labile enterotoxins of Escherichia coli and Vibrio cholerae are classified into two major types. The type I group includes cholera toxin (CT) and E. coli LT-I, whereas the type II subfamily comprises …