Michael Lienemann
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<i>Staphylococcus aureus</i> Adhesion on Hydrophobin Coatings: Adhesion Forces and the Influence of Surface Charge Open
Staphylococcus aureus (S. aureus) is one of the bacterial species capable of forming multilayered biofilms on implants. Such biofilms formed on implanted medical devices often require the removal of the implant in order to avoid sepsis or,…
Stabilization of the catalytically active structure of a molybdenum‐dependent formate dehydrogenase depends on a highly conserved lysine residue Open
Molybdenum‐dependent formate dehydrogenases (Mo‐FDHs) reversibly catalyze the interconversion of CO 2 and formate, and therefore may be utilized for the development of innovative energy storage and CO 2 utilization concepts. Mo‐FDHs contai…
A growth-based screening strategy for engineering the catalytic activity of an oxygen-sensitive formate dehydrogenase Open
Enzyme engineering is a powerful tool for improving or altering the properties of biocatalysts for industrial, research, and therapeutic applications. Fast and accurate screening of variant libraries is often the bottleneck of enzyme engin…
In vitro protein digestion and carbohydrate colon fermentation of microbial biomass samples from bacterial, filamentous fungus and yeast sources Open
This study evaluated the nutritional quality of different microbial biomass samples by assessing their protein digestibility and carbohydrate fermentability in the colon using in vitro methods. Four microbial samples were produced: one hyd…
Hydrophobin Bilayer as Water Impermeable Protein Membrane Open
One of the most important properties of membranes is their permeability to water and other small molecules. A targeted change in permeability allows the passage of molecules to be controlled. Vesicles made of membranes with low water perme…
Production of biopolymer precursors beta-alanine and L-lactic acid from CO2 with metabolically versatile Rhodococcus opacus DSM 43205 Open
Hydrogen oxidizing autotrophic bacteria are promising hosts for conversion of CO 2 into chemicals. In this work, we engineered the metabolically versatile lithoautotrophic bacterium R. opacus strain DSM 43205 for synthesis of polymer precu…
Production of Biopolymer Precursors Beta-Alanine And L-Lactic Acid From CO2 With Metabolically Versatile Rhodococcus Opacus DSM 43205 Open
Background: Hydrogen-oxidizing autotrophic bacteria are promising hosts for conversion of CO 2 into chemicals. In this work, we engineered the metabolically versatile lithoautotrophic bacterium Rhodococcus opacus strain DSM 43205 for synth…
Production of Endotoxin-Free Microbial Biomass for Food Applications by Gas Fermentation of Gram-Positive H<sub>2</sub>-Oxidizing Bacteria Open
The production of edible microbial biomass in bioreactors is an attractive alternative to agriculture, which is a major consumer of fresh water, an emitter of greenhouse gases, and a cause of eutrophication. This study examines microbial f…
Solar-Powered Carbon Fixation for Food and Feed Production Using Microorganisms—A Comparative Techno-Economic Analysis Open
This study evaluates the techno-economic feasibility of five solar-powered concepts for the production of autotrophic microorganisms for food and feed production; the main focus is on three concepts based on hydrogen-oxidizing bacteria (HO…
Production of biopolymer precursors beta-alanine and L-lactic acid from CO <sub>2</sub> with metabolically versatile <i>Rhodococcus opacus</i> DSM 43205 Open
Hydrogen oxidizing autotrophic bacteria are promising hosts for CO 2 conversion into chemicals. In this work, we engineered the metabolically versatile lithoautotrophic bacterium Rhodococcus opacus strain DSM 43205 for synthesis of polymer…
Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic <i>Escherichia coli</i> with heterologous pili Open
Summary Biosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transd…
Adhesion Properties of Freestanding Hydrophobin Bilayers Open
Hydrophobins are a family of small-sized proteins featuring a distinct hydrophobic patch on the protein's surface, rendering them amphiphilic. This particularity allows hydrophobins to self-assemble into monolayers at any hydrophilic/hydro…
Molecular Structure of Hydrophobins Studied with Site-Directed Mutagenesis and Vibrational Sum-Frequency Generation Spectroscopy Open
Hydrophobins are surface-active fungal proteins that adsorb to the water-air interface and self-assemble into amphiphilic, water-repelling films that have a surface elasticity that is an order of magnitude higher than other molecular films…