Michael P. Andreas
YOU?
Author Swipe
View article: A Two‐Component Pseudo‐Icosahedral Protein Nanocompartment with Variable Shell Composition and Irregular Tiling
A Two‐Component Pseudo‐Icosahedral Protein Nanocompartment with Variable Shell Composition and Irregular Tiling Open
Protein shells or capsids are a widespread form of compartmentalization in nature. Viruses use protein capsids to protect and transport their genomes while many cellular organisms use protein shells for varied metabolic purposes. These pro…
View article: HIV-1 binds dynein directly to hijack microtubule transport machinery
HIV-1 binds dynein directly to hijack microtubule transport machinery Open
HIV-1 uses the microtubule cytoskeleton to reach the host cell nucleus during replication, yet the molecular basis for microtubule-dependent HIV-1 motility is poorly understood. Using in vitro reconstitution biochemistry and single-molecul…
View article: Structural and Biochemical Characterization of a Widespread Enterobacterial Peroxidase Encapsulin
Structural and Biochemical Characterization of a Widespread Enterobacterial Peroxidase Encapsulin Open
Encapsulins are self‐assembling protein compartments found in prokaryotes and specifically encapsulate dedicated cargo enzymes. The most abundant encapsulin cargo class are Dye‐decolorizing Peroxidases (DyPs). It has been previously sugges…
View article: Structural and biochemical characterization of a widespread enterobacterial peroxidase encapsulin
Structural and biochemical characterization of a widespread enterobacterial peroxidase encapsulin Open
Encapsulins are self-assembling protein compartments found in prokaryotes and specifically encapsulate dedicated cargo enzymes. The most abundant encapsulin cargo class are Dye-decolorizing Peroxidases (DyPs). It has been previously sugges…
View article: The biosynthesis of the odorant 2-methylisoborneol is compartmentalized inside a protein shell
The biosynthesis of the odorant 2-methylisoborneol is compartmentalized inside a protein shell Open
Terpenoids are the largest class of natural products, found across all domains of life. One of the most abundant bacterial terpenoids is the volatile odorant 2-methylisoborneol (2-MIB), partially responsible for the earthy smell of soil an…
View article: Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages
Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages Open
Protein capsids are a widespread form of compartmentalization in nature. Icosahedral symmetry is ubiquitous in capsids derived from spherical viruses, as this geometry maximizes the internal volume that can be enclosed within. Despite the …
View article: Pore engineering as a general strategy to improve protein-based enzyme nanoreactor performance
Pore engineering as a general strategy to improve protein-based enzyme nanoreactor performance Open
Enzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration and co-localization of enzymes can increase catalytic activity, stability, and longevity, highly desirable…
View article: A two-component quasi-icosahedral protein nanocompartment with variable shell composition and irregular tiling
A two-component quasi-icosahedral protein nanocompartment with variable shell composition and irregular tiling Open
Protein shells or capsids are a widespread form of compartmentalization in nature. Viruses use protein capsids to protect and transport their genomes while many cellular organisms use protein shells for varied metabolic purposes. These pro…
View article: The biosynthesis of the odorant 2-methylisoborneol is compartmentalized inside a protein shell
The biosynthesis of the odorant 2-methylisoborneol is compartmentalized inside a protein shell Open
Terpenoids are the largest class of natural products, found across all domains of life. One of the most abundant bacterial terpenoids is the volatile odorant 2-methylisoborneol (2-MIB), partially responsible for the earthy smell of soil an…
View article: Abstract 2079 Assembly dynamics of a protein nanocompartment with a novel two-component icosahedral architecture
Abstract 2079 Assembly dynamics of a protein nanocompartment with a novel two-component icosahedral architecture Open
Encapsulin nanocompartments are a class of prokaryotic organelles that enable specialized biochemistry through the sequestration of enzymatic cargo within a selectively permeable protein shell. Although encapsulin systems utilize a variety…
View article: Abstract 2418 Enterobacterial encapsulin nanocompartments release their peroxidase cargo under acid stress
Abstract 2418 Enterobacterial encapsulin nanocompartments release their peroxidase cargo under acid stress Open
Intracellular compartmentalization is a fundamental feature of cells. Prokaryotes primarily utilize protein-based nanocompartments such as encapsulin nanocompartments (Enc). A defining feature of encapsulins is their ability to self-assemb…
View article: Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages
Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages Open
Protein capsids are a widespread form of compartmentalisation in nature. Icosahedral symmetry is ubiquitous in capsids derived from spherical viruses, as this geometry maximises the internal volume that can be enclosed within. Despite the …
View article: A widespread bacterial protein compartment sequesters and stores elemental sulfur
A widespread bacterial protein compartment sequesters and stores elemental sulfur Open
Subcellular compartments often serve to store nutrients or sequester labile or toxic compounds. As bacteria mostly do not possess membrane-bound organelles, they often have to rely on protein-based compartments. Encapsulins are one of the …
View article: Cyclodipeptide oxidase is an enzyme filament
Cyclodipeptide oxidase is an enzyme filament Open
Modified cyclic dipeptides represent a widespread class of secondary metabolites with diverse pharmacological activities, including antibacterial, antifungal, and antitumor. Here, we report the structural characterization of the Streptomyc…
View article: Structural basis for peroxidase encapsulation in a protein nanocompartment
Structural basis for peroxidase encapsulation in a protein nanocompartment Open
Encapsulins are self-assembling protein nanocompartments capable of selectively encapsulating dedicated cargo proteins, including enzymes involved in iron storage, sulfur metabolism, and stress resistance. They represent a unique compartme…
View article: HIV-1 binds dynein directly to hijack microtubule transport machinery
HIV-1 binds dynein directly to hijack microtubule transport machinery Open
Summary Viruses exploit host cytoskeletal elements and motor proteins for trafficking through the dense cytoplasm. Yet the molecular mechanism that describes how viruses connect to the motor machinery is unknown. Here, we demonstrate the f…
View article: Structure and heterogeneity of a highly cargo-loaded encapsulin shell
Structure and heterogeneity of a highly cargo-loaded encapsulin shell Open
Encapsulins are self-assembling protein nanocompartments able to selectively encapsulate dedicated cargo enzymes. Encapsulins are widespread across bacterial and archaeal phyla and are involved in oxidative stress resistance, iron storage,…
View article: A widespread proteinaceous sulfur storage compartment in bacteria
A widespread proteinaceous sulfur storage compartment in bacteria Open
Intracellular compartmentalization is essential for all cells and enables the regulation and optimization of metabolism 1 . One of the main functions of subcellular compartments is the storage of nutrients 2–4 . As bacteria do generally no…
View article: Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage
Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage Open
Encapsulins are microbial protein nanocages capable of efficient self-assembly and cargo enzyme encapsulation. Due to their favorable properties, including high thermostability, protease resistance, and robust heterologous expression, enca…
View article: Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage
Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage Open
Encapsulins are protein nanocages capable of efficient self-assembly and cargo enzyme encapsulation. They are found in a wide variety of bacteria and archaea, including many extremophiles, and are involved in iron and sulfur homeostasis, o…
View article: <i>In Vitro</i> Synthesis and Reconstitution Using Mammalian Cell-Free Lysates Enables the Systematic Study of the Regulation of LINC Complex Assembly
<i>In Vitro</i> Synthesis and Reconstitution Using Mammalian Cell-Free Lysates Enables the Systematic Study of the Regulation of LINC Complex Assembly Open
Understanding the structure and structure-function relationships of membrane proteins is a fundamental problem in biomedical research. Given the difficulties inherent to performing mechanistic biochemical and biophysical studies of membran…
View article: Pore structure controls stability and molecular flux in engineered protein cages
Pore structure controls stability and molecular flux in engineered protein cages Open
Protein cages are a common architectural motif used by living organisms to compartmentalize and control biochemical reactions. While engineered protein cages have featured in the construction of nanoreactors and synthetic organelles, relat…
View article: Triggered Reversible Disassembly of an Engineered Protein Nanocage**
Triggered Reversible Disassembly of an Engineered Protein Nanocage** Open
Protein nanocages play crucial roles in sub‐cellular compartmentalization and spatial control in all domains of life and have been used as biomolecular tools for applications in biocatalysis, drug delivery, and bionanotechnology. The abili…
View article: Triggered Reversible Disassembly of an Engineered Protein Nanocage**
Triggered Reversible Disassembly of an Engineered Protein Nanocage** Open
Protein nanocages play crucial roles in sub‐cellular compartmentalization and spatial control in all domains of life and have been used as biomolecular tools for applications in biocatalysis, drug delivery, and bionanotechnology. The abili…