Michael P. Tobin
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View article: Limiting endosomal damage sensing reduces inflammation triggered by lipid nanoparticle endosomal escape
Limiting endosomal damage sensing reduces inflammation triggered by lipid nanoparticle endosomal escape Open
View article: Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia
Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia Open
Macrophages often pervade solid tumors, and clusters of macrophages sometimes associate with longer survival of patients. However, clustering mechanisms and impacts on key functions such as phagocytosis remain obscure. Here, under conditio…
View article: Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia
Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia Open
Macrophages often pervade solid tumors, but their nearest neighbor organization is understudied and potentially enables key functions such as phagocytosis. Here, we observe dynamic macrophage clusters in tumors under conditions that maximi…
View article: Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG
Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG Open
Solid tumors generally exhibit chromosome copy number variation, which is typically caused by chromosomal instability (CIN) in mitosis. The resulting aneuploidy can drive evolution and associates with poor prognosis in various cancer types…
View article: Author response: Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG
Author response: Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG Open
View article: Lipid Nanoparticle-Associated Inflammation is Triggered by Sensing of Endosomal Damage: Engineering Endosomal Escape Without Side Effects
Lipid Nanoparticle-Associated Inflammation is Triggered by Sensing of Endosomal Damage: Engineering Endosomal Escape Without Side Effects Open
Lipid nanoparticles (LNPs) have emerged as the dominant platform for RNA delivery, based on their success in the COVID-19 vaccines and late-stage clinical studies in other indications. However, we and others have shown that LNPs induce sev…
View article: Author Response: Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response
Author Response: Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response Open
Chromosomal instability (CIN), a state in which cells undergo mitotic aberrations that generate chromosome copy number variations, generates aneuploidy and is thought to drive cancer evolution. Although associated with poor prognosis and r…
View article: Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response
Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response Open
Chromosomal instability (CIN), a state in which cells undergo mitotic aberrations that generate chromosome copy number variations, generates aneuploidy and is thought to drive cancer evolution. Although associated with poor prognosis and r…
View article: Differences in cell shape, motility, and growth reflect chromosomal number variations that can be visualized with live-cell ChReporters
Differences in cell shape, motility, and growth reflect chromosomal number variations that can be visualized with live-cell ChReporters Open
Chromosome numbers often change dynamically in tumors and cultured cells, which complicates therapy as well as understanding genotype-mechanotype relationships. Here we use a live-cell “ChReporter” method to identify cells with a single ch…
View article: Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG
Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG Open
Solid tumors generally exhibit chromosome copy number variation, which is typically caused by chromosomal instability (CIN) in mitosis. The resulting aneuploidy can drive evolution and associates with poor prognosis in various cancer types…
View article: Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response
Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response Open
Chromosomal instability (CIN), a state in which cells undergo mitotic aberrations that generate chromosome copy number variations, generates aneuploidy and is thought to drive cancer evolution. Although associated with poor prognosis and r…
View article: Small lipid droplets are rigid enough to indent a nucleus, dilute the lamina, and cause rupture
Small lipid droplets are rigid enough to indent a nucleus, dilute the lamina, and cause rupture Open
The nucleus in many cell types is a stiff organelle, but fat-filled lipid droplets (FDs) in cytoplasm are seen to indent and displace the nucleus. FDs are phase-separated liquids with a poorly understood interfacial tension γ that determin…
View article: Confinement plus myosin-II suppression maximizes heritable loss of chromosomes, as revealed by live-cell ChReporters
Confinement plus myosin-II suppression maximizes heritable loss of chromosomes, as revealed by live-cell ChReporters Open
The mechanical environment of a cell can have many effects, but whether it impacts the DNA sequence of a cell has remained unexamined. To investigate this, we developed a live-cell method to measure changes in chromosome numbers. We edited…
View article: Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response
Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response Open
Chromosomal instability (CIN), a state in which cells undergo mitotic aberrations that generate chromosome copy number variations, generates aneuploidy and is thought to drive cancer evolution. Although associated with poor prognosis and r…
View article: Confinement with Myosin-II suppression increases heritable loss of chromosomes, using live-cell ChReporters
Confinement with Myosin-II suppression increases heritable loss of chromosomes, using live-cell ChReporters Open
Matrix around cells exerts many effects, some of which depend on the putative tumor suppressor Myosin-II, but whether such factors affect DNA sequences in a cell remains unclear. Here, live-cell monitoring of changes to chromosome copy num…
View article: "Small, fat-filled lipid droplets are sufficiently rigid to indent a nucleus, dilute the lamina, and cause rupture"
"Small, fat-filled lipid droplets are sufficiently rigid to indent a nucleus, dilute the lamina, and cause rupture" Open
This is the raw data underlying the Ivanovska, Tobin, et al. paper: "Small, fat-filled lipid droplets are sufficiently rigid to indent a nucleus, dilute the lamina, and cause rupture"
View article: CD47-SIRPα Checkpoint Disruption in Metastases Requires Tumor-Targeting Antibody for Molecular and Engineered Macrophage Therapies
CD47-SIRPα Checkpoint Disruption in Metastases Requires Tumor-Targeting Antibody for Molecular and Engineered Macrophage Therapies Open
The macrophage checkpoint interaction CD47-SIRPα is an emerging target for cancer therapy, but clinical trials of monoclonal anti-CD47 show efficacy only in liquid tumors when combined with tumor-opsonizing IgG. Here, in challenging metast…
View article: Suppressing or Enhancing Macrophage Engulfment through the Use of CD47 and Related Peptides
Suppressing or Enhancing Macrophage Engulfment through the Use of CD47 and Related Peptides Open
Foreign particles and microbes are rapidly cleared by macrophages in vivo, although many key aspects of uptake mechanisms remain unclear. "Self" cells express CD47 which functions as an anti-phagocytic ligand for SIRPα on macrophage…
View article: Gaussian curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate
Gaussian curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate Open
Nuclear rupture has long been associated with deficits or defects in lamins, with recent results also indicating a role for actomyosin stress, but key physical determinants of rupture remain unclear. Here, lamin-B filaments stably interact…
View article: Gaussian curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate
Gaussian curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate Open
Nuclear rupture has long been associated with deficits or defects in lamins, with recent results also indicating a role for actomyosin stress, but key physical determinants of rupture remain unclear. Here, lamin-B filaments stably interact…
View article: CD47-SIRPα checkpoint disruption in metastases requires tumor-targeting antibody for molecular and engineered macrophage therapies
CD47-SIRPα checkpoint disruption in metastases requires tumor-targeting antibody for molecular and engineered macrophage therapies Open
Data for the Cancers (2022) publication: CD47-SIRPα checkpoint disruption in metastases requires tumor-targeting antibody for molecular and engineered macrophage therapies
View article: Data for Gaussian Curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate
Data for Gaussian Curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate Open
Contains the raw data for figures in 2022 manuscript: Gaussian Curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate
View article: Gaussian curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate
Gaussian curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate Open
Nuclear rupture has long been associated with deficits or defects in lamins, with recent results also indicating a role for actomyosin stress, but key physical determinants of rupture remain unclear. Here, lamin-B filaments stably interact…
View article: Gaussian curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate
Gaussian curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate Open
Nuclear rupture has long been associated with deficits or defects in lamins, with recent results also indicating a role for actomyosin stress, but key physical determinants of rupture remain unclear. Here, lamin-B stably interacts with the…
View article: Lipid Droplets Have High Interfafacial Tension and are Sufficiently Rigid to Rupture the Nucleus
Lipid Droplets Have High Interfafacial Tension and are Sufficiently Rigid to Rupture the Nucleus Open
View article: Beyond a Critical Strain Rate, Lamin-A Dilutes and Nuclei Rupture at Sites of High Curvature
Beyond a Critical Strain Rate, Lamin-A Dilutes and Nuclei Rupture at Sites of High Curvature Open
View article: Live Cell Chromosome Loss Reporter Shows Mechanical Confinement Associates with Chromosome Mis-Segregation
Live Cell Chromosome Loss Reporter Shows Mechanical Confinement Associates with Chromosome Mis-Segregation Open
View article: Solid Tumor Cures by Complete Disruption of the CD47:SIRPα Macrophage Checkpoint, Tumor Opsonization, and ‘Phagocytic Feedback’
Solid Tumor Cures by Complete Disruption of the CD47:SIRPα Macrophage Checkpoint, Tumor Opsonization, and ‘Phagocytic Feedback’ Open
View article: Structural analysis of resting mouse platelets by 3D-EM reveals an unexpected variation in α-granule shape
Structural analysis of resting mouse platelets by 3D-EM reveals an unexpected variation in α-granule shape Open
Mice and mouse platelets are major experimental models for hemostasis and thrombosis; however, important physiological data from this model has received little to no quantitative, 3D ultrastructural analysis. We used state-of-the-art, seri…
View article: Beyond a Critical Strain, Lamin-A Dilates and Nuclei Rupture at Sites of High Curvature
Beyond a Critical Strain, Lamin-A Dilates and Nuclei Rupture at Sites of High Curvature Open