Michael J. Poderycki
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View article: CryoAPEX – an electron tomography tool for subcellular localization of membrane proteins
CryoAPEX – an electron tomography tool for subcellular localization of membrane proteins Open
We describe a method, termed cryoAPEX, which couples chemical fixation and high-pressure freezing of cells with peroxidase tagging (APEX) to allow precise localization of membrane proteins in the context of a well-preserved subcellular mem…
View article: CryoAPEX - an electron tomography tool for subcellular localization of membrane proteins
CryoAPEX - an electron tomography tool for subcellular localization of membrane proteins Open
We describe a method, termed cryoAPEX, that couples chemical fixation and high pressure freezing of cells with peroxidase-tagging (APEX) to allow precise localization of membrane proteins in the context of a well-preserved subcellular memb…