Michael R. Watson
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View article: Induction of epigenetic variation in Arabidopsis by over-expression of DNA METHYLTRANSFERASE1 (MET1)
Induction of epigenetic variation in Arabidopsis by over-expression of DNA METHYLTRANSFERASE1 (MET1) Open
Epigenetic marks such as DNA methylation and histone modification can vary among plant accessions creating epi-alleles with different levels of expression competence. Mutations in epigenetic pathway functions are powerful tools to induce e…
View article: <i><scp>TET</scp>3‐</i>mediated demethylation in tomato activates expression of a <i><scp>CETS</scp></i> gene that stimulates vegetative growth
<i><span>TET</span>3‐</i>mediated demethylation in tomato activates expression of a <i><span>CETS</span></i> gene that stimulates vegetative growth Open
Expression of the mammalian DNA demethylase enzyme TET 3 in plants can be used to induce hypomethylation of DNA . In tomato lines that express a TET 3 transgene, we observed distinct phenotypes including an increase in the length and numbe…
View article: An Improved Binary Vector and <i>Escherichia coli</i> Strain for <i>Agrobacterium tumefaciens</i>-Mediated Plant Transformation
An Improved Binary Vector and <i>Escherichia coli</i> Strain for <i>Agrobacterium tumefaciens</i>-Mediated Plant Transformation Open
The plasmid vector pGreenII is widely used to produce plant transformants via a process that involves propagation in Escherichia coli. However, we show here that pGreenII-based constructs can be unstable in E. coli as a consequence of them…
View article: Expression of the C-Terminal Domain of Mammalian &lt;i&gt;TET&lt;/i&gt;3 DNA Dioxygenase in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt; Induces Heritable Methylation Changes at &lt;i&gt;rDNA&lt;/i&gt; Loci
Expression of the C-Terminal Domain of Mammalian <i>TET</i>3 DNA Dioxygenase in <i>Arabidopsis thaliana</i> Induces Heritable Methylation Changes at <i>rDNA</i> Loci Open
In plants, demethylation of 5-methylcytosine (5 mC) residues is controlled by DNA glycosylases, while in mammals it requires oxidation of 5 mC by TET proteins, a group of Fe(II)/2-oxoglutaratedependent dioxygenases. We analysed the effects…
View article: Malware Detection in Cloud Computing Infrastructures
Malware Detection in Cloud Computing Infrastructures Open
Cloud services are prominent within the private, public and commercial domains. Many of these services are expected to be always on and have a critical nature; therefore, security and resilience are increasingly important aspects. In order…