Michael S. Kobor
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View article: Distinct maternal DNA methylation associations with gestational age at early and late-mid term pregnancy in a low- and middle-income country: evaluation of biological, genetic, and psychosocial contributors
Distinct maternal DNA methylation associations with gestational age at early and late-mid term pregnancy in a low- and middle-income country: evaluation of biological, genetic, and psychosocial contributors Open
Mothers undergo physiological and molecular changes over the course of gestation. These modifications “get under the skin” and may be reflected in the maternal epigenome through processes such as DNA methylation. Such an epigenetic mark ma…
View article: Biological sex impacts immune cell proportions and epigenetic profiles in the developing pediatric immune system
Biological sex impacts immune cell proportions and epigenetic profiles in the developing pediatric immune system Open
View article: Evaluation of a DNA methylation-based measure of chronic inflammation in two generations of adults in metropolitan Cebu, Philippines
Evaluation of a DNA methylation-based measure of chronic inflammation in two generations of adults in metropolitan Cebu, Philippines Open
View article: Cross-Platform Methylation-Based Site of Origin Classification for Squamous Cell Carcinomas
Cross-Platform Methylation-Based Site of Origin Classification for Squamous Cell Carcinomas Open
Squamous cell carcinomas (SCCs) are one of the most common cancer types and can arise at nearly any anatomic site. Because SCCs are one of the most common metastases, do not have reliable site-specific morphologic or genomic features, and …
View article: Leaping over the blood-brain barrier: DNA methylation as a link between peripheral and central immune systems
Leaping over the blood-brain barrier: DNA methylation as a link between peripheral and central immune systems Open
The majority of existing DNA methylation (DNAm) studies have used peripheral surrogate tissues to research molecular mechanisms underlying brain-related traits. Epigenetic processes in the brain have yet to be fully elucidated by DNAm find…
View article: The Intersection of Socioeconomic and Environmental Factors in Aging: Insights from a Narrative Review
The Intersection of Socioeconomic and Environmental Factors in Aging: Insights from a Narrative Review Open
(1) Background: Socioeconomic conditions and environmental exposures are well-established determinants of health and aging, yet the pathways through which they influence the aging process remain insufficiently understood. Clarifying these …
View article: Accounting for differences between Infinium MethylationEPIC v2 and v1 in DNA methylation–based tools
Accounting for differences between Infinium MethylationEPIC v2 and v1 in DNA methylation–based tools Open
The recently launched Illumina Infinium MethylationEPIC v2.0 (EPICv2), successor of MethylationEPIC v1.0 (EPICv1), retains most of the probes in EPICv1, while expanding coverage of regulatory elements. The concordance between the two EPIC …
View article: The Intersection of Socioeconomic and Environmental Factors in Aging: Insights from a Narrative Review
The Intersection of Socioeconomic and Environmental Factors in Aging: Insights from a Narrative Review Open
(1) Background: Socioeconomic conditions and environmental exposures are well-established determinants of health and aging, yet the pathways through which they influence the aging process remain insufficiently understood. Clarifying these …
View article: Early-Life Adversity and Epigenetic Aging: Findings from a 17-Year Longitudinal Study
Early-Life Adversity and Epigenetic Aging: Findings from a 17-Year Longitudinal Study Open
Youth exposed to early-life adversity (ELA) are at greater risk for poorer physical and mental health outcomes in adolescence and adulthood. Although the biological mechanisms underlying these associations remain elusive, DNA methylation (…
View article: Complete reference genome and pangenome improve genome-wide detection and interpretation of DNA methylation using sequencing and array data
Complete reference genome and pangenome improve genome-wide detection and interpretation of DNA methylation using sequencing and array data Open
The complete telomere-to-telomere human genome assembly (T2T-CHM13) and the draft human pangenome reference provide unique opportunities to refine DNA methylation (DNAm) studies. Here, we find that T2T-CHM13 calls 7.4% more CpGs genome wid…
View article: Foundations and Strategic Vision of the Canadian Translational Geroscience Network
Foundations and Strategic Vision of the Canadian Translational Geroscience Network Open
Geroscience is an emerging interdisciplinary field that explores the biological connections between aging and the development of chronic diseases, with the ultimate goal of identifying interventions to extend healthspan and delay age-relat…
View article: RAMEN: Dissecting individual, additive and interactive gene-environment contributions to DNA methylome variability in cord blood
RAMEN: Dissecting individual, additive and interactive gene-environment contributions to DNA methylome variability in cord blood Open
DNA methylation (DNAme) is the most commonly studied epigenetic mark in human populations. DNAme has gained attention in the Developmental Origins of Health and Disease field due to its gene expression regulation and potential long-term st…
View article: A Germline Heterozygous Dominant Negative IKZF2 Variant Causing Syndromic Primary Immune Regulatory Disorder and ICHAD
A Germline Heterozygous Dominant Negative IKZF2 Variant Causing Syndromic Primary Immune Regulatory Disorder and ICHAD Open
Monogenic defects that impair the control of inflammation and tolerance lead to profound immune dysregulation, including autoimmunity and atopy. Studying these disorders reveals important molecular and cellular factors that regulate human …
View article: Advancing Pediatric and Longitudinal DNA Methylation Studies with CellsPickMe, an Integrated Blood Cell Deconvolution Method
Advancing Pediatric and Longitudinal DNA Methylation Studies with CellsPickMe, an Integrated Blood Cell Deconvolution Method Open
Prospective birth cohorts offer the potential to interrogate the relation between early life environment and embedded biological processes such as DNA methylation (DNAme). These association studies are frequently conducted in the context o…
View article: Cannabis smoking is associated with persistent epigenome-wide disruptions despite smoking cessation
Cannabis smoking is associated with persistent epigenome-wide disruptions despite smoking cessation Open
Background The use of cannabis has been associated with both therapeutic and harmful effects. As with cigarette smoking, cannabis smoking may affect the epigenetic regulation (e.g., DNA methylation) of gene expression which could result in…
View article: Evaluation of a DNA methylation-based measure of chronic inflammation in two generations of adults in metropolitan Cebu, Philippines
Evaluation of a DNA methylation-based measure of chronic inflammation in two generations of adults in metropolitan Cebu, Philippines Open
Objectives Proxy measures of chronic inflammation derived from DNA methylation (DNAm) data have emerged as promising predictors of cardiometabolic disease risk in high income countries. This study investigates the performance of a recently…
View article: Distinct Co-Methylation Patterns in African and European Populations and Their Genetic Associations
Distinct Co-Methylation Patterns in African and European Populations and Their Genetic Associations Open
Human populations have substantial genetic diversity, but the extent of epigenetic diversity remains unclear, as population-specific DNA methylation (DNAm) has only been studied for ∼3.0% of CpGs. This study quantifies DNAm using whole-gen…
View article: DNA methylation differences between cord and adult white blood cells reflect postnatal immune cell maturation
DNA methylation differences between cord and adult white blood cells reflect postnatal immune cell maturation Open
View article: Examining cellular heterogeneity in human DNA methylation studies: Overview and recommendations
Examining cellular heterogeneity in human DNA methylation studies: Overview and recommendations Open
Intersample cellular heterogeneity (ISCH) is one of the largest contributors to DNA methylation (DNAme) variability. It is imperative to account for ISCH to accurately interpret analysis results in epigenome-wide association studies. We co…
View article: A195 MALNUTRITION IMPAIRS WOUND HEALING DURING DSS COLITIS AND AN IN-VITRO EPITHELIAL MIGRATION ASSAY
A195 MALNUTRITION IMPAIRS WOUND HEALING DURING DSS COLITIS AND AN IN-VITRO EPITHELIAL MIGRATION ASSAY Open
Background Malnutrition is a major public health burden. Caused by insufficient intake of food/nutrients, malnutrition often stems from poverty and lack of access to food. Malnutrition is also a common complication of many gastrointestinal…
View article: A147 CHARACTERIZATION OF EPIGENETIC MODIFICATIONS OF HUMAN COLONOID MONOLAYERS ESTABLISHED AS <i>IN VITRO</i> CHRONIC DAMAGE MODEL
A147 CHARACTERIZATION OF EPIGENETIC MODIFICATIONS OF HUMAN COLONOID MONOLAYERS ESTABLISHED AS <i>IN VITRO</i> CHRONIC DAMAGE MODEL Open
Background Our lab established an in vitro damage model using human colonoids grown as 2D Intestinal Epithelial Cells (IECs) monolayers in Air-Liquid Interface (ALI) culture. Upon repeated injury by submergence, these colonoid monolayers l…
View article: Characterizing the regulatory effects of H2A.Z and SWR1-C on gene expression during hydroxyurea exposure in Saccharomyces cerevisiae
Characterizing the regulatory effects of H2A.Z and SWR1-C on gene expression during hydroxyurea exposure in Saccharomyces cerevisiae Open
Chromatin structure and DNA accessibility are partly modulated by the incorporation of histone variants. H2A.Z, encoded by the non-essential HTZ1 gene in S. cerevisiae , is an evolutionarily conserved H2A histone variant that is predominan…
View article: Impact of age-related changes in buccal epithelial cells on pediatric epigenetic biomarker research
Impact of age-related changes in buccal epithelial cells on pediatric epigenetic biomarker research Open
Cheek swabs, heterogeneous samples consisting primarily of buccal epithelial cells, are widely used in pediatric DNA methylation studies and biomarker creation. However, the decrease in buccal proportion with age in adults remains unexamin…
View article: Epigenome-wide association study of placental co-methylated regions in newborns for prenatal opioid exposure
Epigenome-wide association study of placental co-methylated regions in newborns for prenatal opioid exposure Open
The increasing incidence of opioid use during pregnancy has led to a rise in the number of infants exposed to opioids in utero. Prenatal opioid exposure may have consequences for health and (neuro)development, including neonatal opioid wit…
View article: Technical variability across the 450K, EPICv1, and EPICv2 DNA methylation arrays: lessons learned for clinical and longitudinal studies
Technical variability across the 450K, EPICv1, and EPICv2 DNA methylation arrays: lessons learned for clinical and longitudinal studies Open
DNA methylation (DNAm) is the most commonly measured epigenetic mechanism in human populations, with most studies using Illumina arrays to assess DNAm levels. In 2023, Illumina updated their DNAm arrays to the EPIC version 2 (EPICv2), buil…
View article: Biological sex impacts immune cell proportions and epigenetic profiles in the developing pediatric immune system
Biological sex impacts immune cell proportions and epigenetic profiles in the developing pediatric immune system Open
Age- and sex-related differences in immune cell compositions and immune outcomes have been identified in adult and aging populations, but a comprehensive and nuanced characterization of these changes during the rapid developmental window o…
View article: Not All Saliva Samples Are Equal: The Role of Cellular Heterogeneity in DNA methylation and Epigenetic Age Analyses with Biological and Psychosocial Factors
Not All Saliva Samples Are Equal: The Role of Cellular Heterogeneity in DNA methylation and Epigenetic Age Analyses with Biological and Psychosocial Factors Open
Saliva is widely used in biomedical population research, including epigenetic analyses to investigate gene-environment interplay and identify biomarkers. Its minimally invasive collection procedure makes it ideal for studies in pediatric p…
View article: Genetic Basis, Quantitative Nature, and Functional Relevance of Evolutionarily Conserved DNA Methylation
Genetic Basis, Quantitative Nature, and Functional Relevance of Evolutionarily Conserved DNA Methylation Open
DNA methylation (DNAm) is a key epigenetic mark that modulates regulatory elements and gene expression, playing a crucial role in mammalian development and physiological function. Despite extensive characterization of DNAm profiles across …
View article: Association between maternal perinatal stress and depression and infant DNA methylation in the first year of life
Association between maternal perinatal stress and depression and infant DNA methylation in the first year of life Open
Maternal stress and depression during pregnancy and the first year of the infant's life affect a large percentage of mothers. Maternal stress and depression have been associated with adverse fetal and childhood outcomes as well as differen…
View article: From fetus to 8: the CHILD Cohort Study
From fetus to 8: the CHILD Cohort Study Open
The CHILD Cohort Study is an active multicenter, longitudinal, prospective, population pregnancy cohort study following Canadian infants from fetal life until adulthood. We hypothesized that early life physical and psychosocial environment…