Michael Schroda
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CurT/CURT1 proteins are involved in cell and chloroplast division coordination of cyanobacteria and green algae Open
Plant proteins of the CURVATURE THYLAKOID 1 (CURT1) family and their prokaryotic CurT homologues are key determinants of the three-dimensional structure of the thylakoid membrane systems in chloroplasts and cyanobacteria. As the evolutiona…
The proxiomes of CDJ5 and PGRL1 in Chlamydomonas reinhardtii overlap depending on the ability of CDJ5 to bind a 4Fe-4S cluster Open
The chloroplast chaperone HSP70B from Chlamydomonas reinhardtii works with the J-domain co-chaperones CDJ1 to CDJ6. CDJ1 delivers unfolded proteins, while CDJ2 delivers VIPP1 as substrate to HSP70B. CDJ3 to CDJ5 contain 4Fe-4S clusters in …
View article: Complexome profiling of the Chlamydomonas <i>psb28</i> mutant reveals TEF5 as an early PSII assembly factor
Complexome profiling of the Chlamydomonas <i>psb28</i> mutant reveals TEF5 as an early PSII assembly factor Open
PSII assembly requires auxiliary factors, including Psb28. Although the absence of Psb28 in cyanobacteria has little effect on PSII assembly, we show here that the Chlamydomonas (Chlamydomonas reinhardtii) psb28 null mutant is severely imp…
Unfolded protein responses in <i>Chlamydomonas reinhardtii</i> Open
The disruption of protein homeostasis leads to the increased un- and misfolding of proteins and the formation of toxic protein aggregates. Their accumulation triggers an unfolded protein response that is characterized by the transcriptiona…
Purification and Electron Transfer from Soluble c-Type Cytochrome TorC to TorA for Trimethylamine N-Oxide Reduction Open
The enterobacterium Escherichia coli present in the human gut can reduce trimethylamine N-oxide (TMAO) to trimethylamine during anaerobic respiration. The TMAO reductase TorA is a monomeric, bis-molybdopterin guanine dinucleotide (bis-MGD)…
View article: Complexome profiling of the Chlamydomonas <i>psb28</i> mutant reveals THYLAKOID ENRICHED FRACTION 5 as an early photosystem II assembly factor
Complexome profiling of the Chlamydomonas <i>psb28</i> mutant reveals THYLAKOID ENRICHED FRACTION 5 as an early photosystem II assembly factor Open
Several auxiliary factors are required for the assembly of photosystem (PS) II, one of which is Psb28. While the absence of Psb28 in cyanobacteria has little effect on PSII assembly, we show here that the Chlamydomonas psb28 -null mutant i…
A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae Open
Modular Cloning (MoClo) is based on libraries of standardized genetic parts that can be directionally assembled via Golden Gate cloning in one-pot reactions into transcription units and multigene constructs. Here, a team of bachelor studen…
Structure, biogenesis, and evolution of thylakoid membranes Open
Cyanobacteria and chloroplasts of algae and plants harbor specialized thylakoid membranes (TMs) that convert sunlight into chemical energy. These membranes house PSII and I, the vital protein-pigment complexes that drive oxygenic photosynt…
Genome Comparisons between Botrytis fabae and the Closely Related Gray Mold Fungus Botrytis cinerea Reveal Possible Explanations for Their Contrasting Host Ranges Open
While Botrytis cinerea causes gray mold on many plants, its close relative, Botrytis fabae, is host-specifically infecting predominantly faba bean plants. To explore the basis for its narrow host range, a gapless genome sequence of B. faba…
Das ESCRT-III-Homolog VIPP1 vermittelt Thylakoid-Biogenese und -Erhaltung Open
At thylakoid membranes the light reactions of oxygenic photosynthesis take place, which are the source of virtually all life on earth. The VIPP1 protein is essential for the biogenesis and maintenance of thylakoid membranes. Recently, the …
View article: Degradation of FATTY ACID EXPORT PROTEIN1 by RHOMBOID-LIKE PROTEASE11 contributes to cold tolerance in Arabidopsis
Degradation of FATTY ACID EXPORT PROTEIN1 by RHOMBOID-LIKE PROTEASE11 contributes to cold tolerance in Arabidopsis Open
Plants need to acclimate to different stresses to optimize growth under unfavorable conditions. In Arabidopsis (Arabidopsis thaliana), the abundance of the chloroplast envelope protein FATTY ACID EXPORT PROTEIN1 (FAX1) decreases after the …
View article: The impact of light and thioredoxins on the plant thiol-disulfide proteome
The impact of light and thioredoxins on the plant thiol-disulfide proteome Open
Thiol-based redox regulation is a crucial posttranslational mechanism to acclimate plants to changing light availability. Here, we conducted a biotin switch–based redox proteomics study in Arabidopsis (Arabidopsis thaliana) to systematical…
Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in <i>Synechocystis</i> Open
Phycobiliproteins (PBPs) play a vital role in light harvesting by cyanobacteria, which enables efficient utilization of photon energy for oxygenic photosynthesis. The PBPs carry phycobilins, open‐chain tetrapyrrole chromophores derived fro…
View article: Degradation of Fatty Acid Export Protein1 by Rhomboid-Like Protease11 Contributes to Cold Tolerance in Arabidopsis
Degradation of Fatty Acid Export Protein1 by Rhomboid-Like Protease11 Contributes to Cold Tolerance in Arabidopsis Open
Plants need to adapt to different stresses to optimize growth under unfavorable conditions. The abundance of the chloroplast envelope located Fatty Acid Export Protein1 (FAX1) decreases after the onset of low temperatures. However, it was …
View article: The impact of light and thioredoxins on the plant thiol-disulfide proteome
The impact of light and thioredoxins on the plant thiol-disulfide proteome Open
Thiol-based redox regulation is a crucial post-translational mechanism to acclimate plants to changing light availability. Here, we conduct a biotin-switch-based redox proteomics study to systematically investigate dynamics of the thiol-re…
Networked proteins redundantly interact with VAP27 and RABG3 to regulate membrane tethering at the vacuole and beyond Open
Biological processes in eukaryotes depend on the spatio-temporal compartmentalization of their cells. Integrity and positioning of organelles on the other hand rely on the organization of the actin cytoskeleton. Previously, it has been sho…
Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in <i>Synechocystis</i> Open
Phycobiliproteins (PBPs) play a vital role in light harvesting by cyanobacteria, which enables efficient utilization of photon energy for oxygenic photosynthesis. The PBPs carry phycobilins, open-chain tetrapyrrole chromophores derived fro…
Competition co‐immunoprecipitation reveals the interactors of the chloroplast CPN60 chaperonin machinery Open
The functionality of all metabolic processes in chloroplasts depends on a balanced integration of nuclear‐ and chloroplast‐encoded polypeptides into the plastid's proteome. The chloroplast chaperonin machinery is an essential player in chl…
TurboID reveals the proxiomes of<i>Chlamydomonas</i>proteins involved in thylakoid biogenesis and stress response Open
In Chlamydomonas (Chlamydomonas reinhardtii), the VESICLE-INDUCING PROTEIN IN PLASTIDS 1 and 2 (VIPP1 and VIPP2) play roles in the sensing and coping with membrane stress and in thylakoid membrane biogenesis. To gain more insight into thes…
Analysis of Viral Promoters for Transgene Expression and of the Effect of 5′-UTRs on Alternative Translational Start Sites in Chlamydomonas Open
Microalgae biotechnology has the potential to produce high quality bioproducts in a sustainable manner. Here, Chlamydomonas reinhardtii has shown great potential as a host for biotechnological exploitation. However, low expression of nucle…
CLPB3 is required for the removal of chloroplast protein aggregates and thermotolerance in <i>Chlamydomonas</i> Open
In the cytosol of plant cells, heat-induced protein aggregates are resolved by the CASEIN LYTIC PROTEINASE/HEAT SHOCK PROTEIN 100 (CLP/HSP100) chaperone family member HSP101, which is essential for thermotolerance. For the chloroplast fami…
One-helix protein 2 is not required for the synthesis of photosystem II subunit D1 in Chlamydomonas Open
In land plants and cyanobacteria, co-translational association of chlorophyll (Chl) to the nascent D1 polypeptide, a reaction center protein of photosystem II (PSII), requires a Chl binding complex consisting of a short-chain dehydrogenase…
Competition co-immunoprecipitation reveals interactors of the chloroplast CPN60 chaperonin machinery Open
SUMMARY The functionality of essential metabolic processes in chloroplasts depends on a balanced integration of nuclear-and chloroplast-encoded polypeptides into the plastid’s proteome. The chloroplast chaperonin machinery is an essential …
Engineered green alga <i>Chlamydomonas reinhardtii</i> as a whole-cell photosynthetic biocatalyst for stepwise photoproduction of H<sub>2</sub> and ε-caprolactone Open
Photosynthetic whole-cell biocatalysts are promising platforms for direct production of solar chemicals.
TurboID reveals the proxiomes of CGE1, VIPP1, and VIPP2 in <i>Chlamydomonas reinhardtii</i> Open
In Chlamydomonas reinhardtii , VIPP1 and VIPP2 play a role in the sensing and coping with membrane stress and in thylakoid membrane biogenesis. To gain more insight into these processes, we aimed to identify proteins interacting with VIPP1…
A Cyanophage MarR-Type Transcription Factor Regulates Host RNase E Expression during Infection Open
The marine picocyanobacterium Prochlorococcus contributes significantly to global primary production, and its abundance and diversity is shaped in part by viral infection. Here, we identified a cyanophage-encoded MarR-type transcription fa…
CLPB3 is required for the removal of chloroplast protein aggregates and for thermotolerance in Chlamydomonas Open
In the cytosol of plant cells, heat-induced protein aggregates are resolved by ClpB/Hsp100 family member HSP101, which is essential for thermotolerance. For chloroplast family member CLPB3 this is less clear with controversial reports on i…