Michael See
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View article: The DLX/Notch axis is necessary for spatiotemporal regulation of neural cell fate
The DLX/Notch axis is necessary for spatiotemporal regulation of neural cell fate Open
The neuronal-glial cell fate switch during forebrain development is highly regulated. DLX transcription factors are necessary for promoting GABAergic interneuron differentiation and migration but the mechanisms for concomitant repression o…
View article: Procollagen IIA mediates positive feedback control of the mouse cardiogenic transcriptional network
Procollagen IIA mediates positive feedback control of the mouse cardiogenic transcriptional network Open
Cardiogenesis relies on the integrated interplay between cardiac transcription factors and signaling pathways. Here, we uncover a role for type IIA procollagen (IIA), an extracellular matrix (ECM) protein encoded by an alternatively splice…
View article: <i>ACTN3</i> genotype influences androgen response in developing murine skeletal muscle
<i>ACTN3</i> genotype influences androgen response in developing murine skeletal muscle Open
Androgens act through androgen receptor (AR) to maintain muscle mass. Evidence suggests that this pathway is influenced by “the gene for speed,” ACTN3 (α-actinin-3). Given that one in five people lack α-actinin-3, it is possible that they …
View article: Efficient installation of heterozygous mutations in human pluripotent stem cells using prime editing
Efficient installation of heterozygous mutations in human pluripotent stem cells using prime editing Open
SUMMARY The utility of human pluripotent stem cells (hPSCs) is greatly enhanced by the ability to introduce precise, site-specific genetic modifications with minimal off-target effects. Although Cas9 endonuclease is an exceptionally effici…
View article: Nkx2.7 is a conserved regulator of craniofacial development
Nkx2.7 is a conserved regulator of craniofacial development Open
Craniofacial malformations arise from developmental defects in the head, face, and neck with phenotypes such as 22q11.2 deletion syndrome illustrating a developmental link between cardiovascular and craniofacial morphogenesis. NKX2-5 is a …
View article: Macrophages Mediate Antiviral Immunity and Repair of Type 2 Alveolar Epithelial Cells in a Human Stem Cell Model
Macrophages Mediate Antiviral Immunity and Repair of Type 2 Alveolar Epithelial Cells in a Human Stem Cell Model Open
The lung alveoli are constantly exposed to inhaled pathogens and inorganic hazards, relying on robust defence mechanisms to maintain homeostasis. Alveolar macrophages and type 2 alveolar epithelial cells (AT2s) collaborate to orchestrate p…
View article: Active maintenance of CD8<sup>+</sup>T cell naïvety through regulation of global genome architecture
Active maintenance of CD8<sup>+</sup>T cell naïvety through regulation of global genome architecture Open
SUMMARY The differentiation of naïve CD8 + cytotoxic T lymphocytes (CTLs) into effector and memory states results in large scale changes in transcriptional and phenotypic profiles. Little is known about how large-scale changes in genome or…
View article: Kidney Organoids Generated Using an Allelic Series of NPHS2 Point Variants Reveal Distinct Intracellular Podocin Mistrafficking
Kidney Organoids Generated Using an Allelic Series of NPHS2 Point Variants Reveal Distinct Intracellular Podocin Mistrafficking Open
Significance Statement Missense variants of NPHS2 that cause mistrafficking of the encoded protein, PODOCIN, have been associated with steroid-resistant nephrotic syndrome. However, most studies have overexpressed such variants in 2D nonpo…
View article: Parallel use of pluripotent human stem cell lung and heart models provide new insights for treatment of SARS-CoV-2
Parallel use of pluripotent human stem cell lung and heart models provide new insights for treatment of SARS-CoV-2 Open
SARS-CoV-2 primarily infects the respiratory tract, but pulmonary and cardiac complications occur in severe COVID-19. To elucidate molecular mechanisms in the lung and heart, we conducted paired experiments in human stem cell-derived lung …
View article: Human pluripotent stem cell-derived macrophages host Mycobacterium abscessus infection
Human pluripotent stem cell-derived macrophages host Mycobacterium abscessus infection Open
Human macrophages are a natural host of many mycobacterium species, including Mycobacterium abscessus (M. abscessus), an emerging pathogen affecting immunocompromised and cystic fibrosis patients with few available treatments. The search f…
View article: Genetic and pharmacological evidence for kinetic competition between alternative poly(A) sites in yeast
Genetic and pharmacological evidence for kinetic competition between alternative poly(A) sites in yeast Open
Most eukaryotic mRNAs accommodate alternative sites of poly(A) addition in the 3’ untranslated region in order to regulate mRNA function. Here, we present a systematic analysis of 3’ end formation factors, which revealed 3’UTR lengthening …
View article: The human<i>FLT1</i>regulatory element directs vascular expression and modulates angiogenesis pathways<i>in vitro</i>and<i>in vivo</i>
The human<i>FLT1</i>regulatory element directs vascular expression and modulates angiogenesis pathways<i>in vitro</i>and<i>in vivo</i> Open
There is growing evidence that mutations in non-coding cis -regulatory elements (CREs) disrupt proper development. However, little is known about human CREs that are crucial for cardiovascular development. To address this, we bioinformatic…
View article: Additional file 2 of A cis-regulatory-directed pipeline for the identification of genes involved in cardiac development and disease
Additional file 2 of A cis-regulatory-directed pipeline for the identification of genes involved in cardiac development and disease Open
Additional file 2. List of genes associated with both cardiac-specific promoters and enhancers.
View article: Genetic and pharmacological evidence for kinetic competition between alternative poly(A) sites in yeast
Genetic and pharmacological evidence for kinetic competition between alternative poly(A) sites in yeast Open
Most eukaryotic mRNAs accommodate alternative sites of poly(A) addition in the 3’ untranslated region in order to regulate mRNA function. Here we present a systematic analysis of 3’ end formation factors, which revealed 3’UTR lengthening i…
View article: The YEATS Domain Histone Crotonylation Readers Control Virulence-Related Biology of a Major Human Pathogen
The YEATS Domain Histone Crotonylation Readers Control Virulence-Related Biology of a Major Human Pathogen Open
Identification of multiple histone acylations diversifies transcriptional control by metabolism, but their functions are incompletely defined. Here we report evidence of histone crotonylation in the human fungal pathogen Candida albicans. …
View article: Coordination of Cell Cycle Progression and Mitotic Spindle Assembly Involves Histone H3 Lysine 4 Methylation by Set1/COMPASS
Coordination of Cell Cycle Progression and Mitotic Spindle Assembly Involves Histone H3 Lysine 4 Methylation by Set1/COMPASS Open
Methylation of histone H3 lysine 4 (H3K4) by Set1 complex/COMPASS is a hallmark of eukaryotic chromatin, but it remains poorly understood how this post-translational modification contributes to the regulation of biological processes like t…