Michal Franek
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View article: The histone chaperones <scp>ASF1</scp> and <scp>HIRA</scp> are required for telomere length and <scp>45S rDNA</scp> copy number homeostasis
The histone chaperones <span>ASF1</span> and <span>HIRA</span> are required for telomere length and <span>45S rDNA</span> copy number homeostasis Open
SUMMARY Genome stability is significantly influenced by the precise coordination of chromatin complexes that facilitate the loading and eviction of histones from chromatin during replication, transcription, and DNA repair processes. In thi…
View article: Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications
Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications Open
View article: In-section Click-iT detection and super-resolution CLEM analysis of nucleolar ultrastructure and replication in plants
In-section Click-iT detection and super-resolution CLEM analysis of nucleolar ultrastructure and replication in plants Open
Correlative light and electron microscopy (CLEM) is an important tool for the localisation of target molecule(s) and their spatial correlation with the ultrastructural map of subcellular features at the nanometre scale. Adoption of these a…
View article: Telomerase <scp>RNA</scp> gene paralogs in plants – the usual pathway to unusual telomeres
Telomerase <span>RNA</span> gene paralogs in plants – the usual pathway to unusual telomeres Open
Summary Telomerase, telomeric DNA and associated proteins together represent a complex, finely tuned and functionally conserved mechanism that ensures genome integrity by protecting and maintaining chromosome ends. Changes in its component…
View article: In-section Click-iT detection and super-resolution CLEM: Shedding light on nucleolar ultrastructure and S-phase progression in plants
In-section Click-iT detection and super-resolution CLEM: Shedding light on nucleolar ultrastructure and S-phase progression in plants Open
Correlative light and electron microscopy (CLEM) is an essential tool that allows for localisation of a particular target molecule(s) and their spatial correlation with the ultrastructural map of subcellular features at the nanometer scale…
View article: Telomerase RNA in Hymenoptera (Insecta) switched to plant/ciliate-like biogenesis
Telomerase RNA in Hymenoptera (Insecta) switched to plant/ciliate-like biogenesis Open
In contrast to the catalytic subunit of telomerase, its RNA subunit (TR) is highly divergent in size, sequence and biogenesis pathways across eukaryotes. Current views on TR evolution assume a common origin of TRs transcribed with RNA poly…
View article: Hymenoptera (Insecta) telomerase RNAs switched to plant/ciliate-like biogenesis
Hymenoptera (Insecta) telomerase RNAs switched to plant/ciliate-like biogenesis Open
In contrast to the catalytic subunit of telomerase, its RNA subunit (TR) is highly divergent in size, sequence and biogenesis pathways across eukaryotes. Current views on TR evolution assume a common origin of TRs transcribed with RNA poly…
View article: Laser microirradiation as a versatile system for probing protein recruitment and protein–protein interactions at DNA lesions in plants
Laser microirradiation as a versatile system for probing protein recruitment and protein–protein interactions at DNA lesions in plants Open
Summary Plant protoplasts are generated by treatment with digestion enzymes, producing plant cells devoid of the cell wall and competent for efficient polyethylene glycol mediated transformation. This way fluorescently tagged proteins can …
View article: Super-resolution microscopy of chromatin fibers and quantitative DNA methylation analysis of DNA fiber preparations
Super-resolution microscopy of chromatin fibers and quantitative DNA methylation analysis of DNA fiber preparations Open
Analysis of histone variants and epigenetic marks is dominated by genome-wide approaches in the form of chromatin immunoprecipitation-sequencing (ChIP-seq) and related methods. Although uncontested in their value for single-copy genes, map…
View article: Nucleolar rDNA folds into condensed foci with a specific combination of epigenetic marks
Nucleolar rDNA folds into condensed foci with a specific combination of epigenetic marks Open
SUMMARY Arabidopsis thaliana 45S ribosomal genes (rDNA) are located in tandem arrays called nucleolus organizing regions on the termini of chromosomes 2 and 4 (NOR2 and NOR4) and encode rRNA, a crucial structural element of the ribosome. T…
View article: Large tandem duplications affect gene expression, 3D organization, and plant–pathogen response
Large tandem duplications affect gene expression, 3D organization, and plant–pathogen response Open
Rapid plant genome evolution is crucial to adapt to environmental changes. Chromosomal rearrangements and gene copy number variation (CNV) are two important tools for genome evolution and sources for the creation of new genes. However, the…
View article: Multiple epigenetic layers accompany the spatial distribution of ribosomal genes in Arabidopsis
Multiple epigenetic layers accompany the spatial distribution of ribosomal genes in Arabidopsis Open
45S ribosomal genes in A. thaliana (rDNA) are located in tandem arrays on termini of chromosomes 2 and 4 (NOR2 and NOR4) and encode rRNA, crucial structural elements of the ribosome. Inactive rDNA genes accumulate in the condensed chromoce…
View article: Probing the chromatin structure of ribosomal DNA using extended chromatin fibers
Probing the chromatin structure of ribosomal DNA using extended chromatin fibers Open
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View article: Roles of <scp>RAD</scp>51 and <scp>RTEL</scp>1 in telomere and <scp>rDNA</scp> stability in <i>Physcomitrella patens</i>
Roles of <span>RAD</span>51 and <span>RTEL</span>1 in telomere and <span>rDNA</span> stability in <i>Physcomitrella patens</i> Open
Summary Telomeres and ribosomal RNA genes ( rDNA ) are essential for cell survival and particularly sensitive to factors affecting genome stability. Here, we examine the role of RAD 51 and its antagonist, RTEL 1, in the moss Physcomitrella…
View article: Description of the most important wars, which took place on Balkan territory
Description of the most important wars, which took place on Balkan territory Open
Purpose and Originality: The article is overview of Balkan changing wars. Balkan is historical area of conflict, however there are wars, which should be singled out due to their influence on the territory as well as on the population and p…
View article: Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction
Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction Open
DNA damage response (DDR) in ribosomal genes and mechanisms of DNA repair in embryonic stem cells (ESCs) are less explored nuclear events. DDR in ESCs should be unique due to their high proliferation rate, expression of pluripotency factor…
View article: CARM1 Modulators Affect Epigenome of Stem Cells and Change Morphology of Nucleoli
CARM1 Modulators Affect Epigenome of Stem Cells and Change Morphology of Nucleoli Open
CARM1 interacts with numerous transcription factors to mediate cellular processes, especially gene expression. This is important for the maintenance of ESC pluripotency or intervention to tumorigenesis. Here, we studied epigenomic effects …