Michal Ješeta
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View article: Assisted Reproduction Therapy in Patients with Multiple Sclerosis: Narrative Review and Practical Recommendations
Assisted Reproduction Therapy in Patients with Multiple Sclerosis: Narrative Review and Practical Recommendations Open
Objective: The objective of this study is to present contemporary findings regarding the relationship between the application of assisted reproduction methods and their impact on the incidence of multiple sclerosis. Design: This study adop…
View article: Comparison of Swim-Up and Microfluidic Sperm Sorting Methods in Selection of Sperm for Intracytoplasmic Sperm Injection
Comparison of Swim-Up and Microfluidic Sperm Sorting Methods in Selection of Sperm for Intracytoplasmic Sperm Injection Open
The use of microfluidic sperm sorting (MFSS) systems in infertility treatment is increasing due to their practicality and ease of use. While often presented as highly effective, their efficacy in patients with varying sperm analysis result…
View article: Efficiency of sperm separation by using microfluidic chips compared to the swim up method
Efficiency of sperm separation by using microfluidic chips compared to the swim up method Open
The effectiveness of assisted reproduction methods is still not as high as was expected recently. For effective therapy, it is necessary to use only the highest quality sperm for oocyte fertilization, as the fertilization process most ofte…
View article: Morphological characteristics, extracellular vesicle structure and stem-like specificity of human follicular fluid cell subpopulation during osteodifferentiation
Morphological characteristics, extracellular vesicle structure and stem-like specificity of human follicular fluid cell subpopulation during osteodifferentiation Open
Extracellular vesicles can play an important role in the processes occurring after stem cell transplantation, preventing cell apoptosis, stimulating immunological processes, and promoting the synthesis of extracellular matrix. Human follic…
View article: Comparison of Swim-Up and MFSS Methods in Selection of Sperm for ICSI
Comparison of Swim-Up and MFSS Methods in Selection of Sperm for ICSI Open
Microfluidic sperm selection systems (MFSS) are increasingly being used and appear to be a promising adjunct in assisted reproductive treatment (ART). They are practical, easy to use, and often presented as highly effective. In this study,…
View article: Bioenergetics of human spermatozoa in patients with testicular germ cell tumour
Bioenergetics of human spermatozoa in patients with testicular germ cell tumour Open
In testicular germ cell tumour (TGCT) patients, sperm cryopreservation prior to anti-cancer treatment represents the main fertility preservation approach. However, it is associated with low sperm recovery rate after thawing. Since sperm is…
View article: Cross sectional study on exposure to BPA and its analogues and semen parameters in Czech men
Cross sectional study on exposure to BPA and its analogues and semen parameters in Czech men Open
View article: Morphological Characteristic, Extracellular Vesicles Structure and Stemness Specificity of Human Follicular Fluid Cells During Osteodifferentiation
Morphological Characteristic, Extracellular Vesicles Structure and Stemness Specificity of Human Follicular Fluid Cells During Osteodifferentiation Open
View article: Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells Open
View article: Immediate Breast Reconstruction After Mastectomy from the Patient's Point of View
Immediate Breast Reconstruction After Mastectomy from the Patient's Point of View Open
1) Objective: Breast cancer is the most common cancer in women, and the incidence of the disease continues to increase.The issue of immediate breast reconstruction (IBR) in women with BRCA mutations and breast cancer is highly topical.This…
View article: Non-Invasive Diagnostics of Male Spermatogenesis from Seminal Plasma: Seminal Proteins
Non-Invasive Diagnostics of Male Spermatogenesis from Seminal Plasma: Seminal Proteins Open
The compounds of seminal plasma have great potential as biomarkers of male fertility and can be used as a diagnostic tool for types of azoospermia. Azoospermia occurs in approximately 1% of the male population, and for an effective therapy…
View article: Czech Women’s Point of Views on Immediate Breast Reconstruction after Mastectomy due to BRCA Gene Mutation or Breast Cancer
Czech Women’s Point of Views on Immediate Breast Reconstruction after Mastectomy due to BRCA Gene Mutation or Breast Cancer Open
(1) Objective: Breast cancer is the most common cancer in women, and the incidence of the disease continues to increase. The issue of immediate breast reconstruction (IBR) in women with BRCA mutations and breast cancer is highly topical. T…
View article: The bisphenol S contamination level observed in human follicular fluid affects the development of porcine oocytes
The bisphenol S contamination level observed in human follicular fluid affects the development of porcine oocytes Open
Bisphenol S (BPS), the main replacement for bisphenol A (BPA), is thought to be toxic, but limited information is available on the effects of Bisphenol S on ovarian follicles. In our study, we demonstrated the presence of Bisphenol S in th…
View article: The contribution of donated human embryos suitable for the production of embryonic stem cells to increase the quality of life: Selection and preparation of embryos in the Czech Republic
The contribution of donated human embryos suitable for the production of embryonic stem cells to increase the quality of life: Selection and preparation of embryos in the Czech Republic Open
We established a current good manufacturing practice (cGMP)-defined xeno-free and feeder-free system for the derivation, culture and banking of clinical-grade hESC lines that are suitable for preclinical and clinical trials. The quality co…
View article: Proteome Mapping of Cervical Mucus and Its Potential as a Source of Biomarkers in Female Tract Disorders
Proteome Mapping of Cervical Mucus and Its Potential as a Source of Biomarkers in Female Tract Disorders Open
Cervical mucus (CM) is a viscous fluid that is produced by the cervical glands and functions as a uterine cervix plug. Its viscosity decreases during ovulation, providing a window for non-invasive sampling. This study focuses on proteomic …
View article: The Manufacture of Xeno- and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy
The Manufacture of Xeno- and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy Open
Human embryonic stem cells (hESCs) are increasingly used in clinical trials as they can change the outcome of treatment for many human diseases. They are used as a starting material for further differentiation into specific cell types and …
View article: Correlation between per- and polyfluoroalkyl substances (PFAS) in seminal plasma and semen quality parameters in firefighters
Correlation between per- and polyfluoroalkyl substances (PFAS) in seminal plasma and semen quality parameters in firefighters Open
BACKGROUND AND AIM For decades firefighters have used aqueous film-forming foam (AFFF) to extinguish specific types of fires. Some of the AFFF formulations contains per- and polyfluoroalkyl substances (PFASs). PFASs exposure was reported t…
View article: The contribution of the donated human embryos suitable for production of embryonic stem cells to increase the quality of life: selection and preparation of embryos in Czech Republic
The contribution of the donated human embryos suitable for production of embryonic stem cells to increase the quality of life: selection and preparation of embryos in Czech Republic Open
Human embryonic stem cells (hESCs) are used in cell therapy mainly in targeted therapy for not yet curable diseases such as diabetes mellitus, spinal cord injury or Parkinson's disease. The aim of this study is the derivation of clinical-g…
View article: P-067 Correlation between Zn/Cu ratio and Bisphenol A in seminal plasma in men with varicocele
P-067 Correlation between Zn/Cu ratio and Bisphenol A in seminal plasma in men with varicocele Open
Study question Is there a relationship between concentration of Bisphenol A (BPA), Zn contents and Zn/Cu ratio in seminal fluid and spermiogram parameters in men with varicocele? Summary answer Our results document correlations between BPA…
View article: P-042 Method validation for a multi-element panel in human seminal plasma by inductively coupled plasma tandem mass spectrometry (ICP-MS/MS)
P-042 Method validation for a multi-element panel in human seminal plasma by inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) Open
Study question Can we quantitatively, accurately, and simultaneously determine concentrations of essential and toxic elements in seminal fluid? Summary answer We developed and validated a multi-element panel to quantify essential and toxic…
View article: The Bisphenols Found in the Ejaculate of Men Does Not Pass through the Testes
The Bisphenols Found in the Ejaculate of Men Does Not Pass through the Testes Open
Exposure to bisphenols is related to negative effects on male reproduction. The bisphenols exposure is associated with several modes of action including negative impact on the blood–testis barrier (BTB) in testes or direct effect on sperma…
View article: Approaches for in vitro culture of granulosa cells and ovarian follicles
Approaches for in vitro culture of granulosa cells and ovarian follicles Open
The in vitro culture of ovarian follicles or cumulus-oocyte complexes (COC) is used to study the factors that regulate follicular development and may have potential use in artificial reproductive technology (ART). Before ovulation, the fol…
View article: Viability of bovine in vitro matured oocytes following ultra-rapid vitrification
Viability of bovine in vitro matured oocytes following ultra-rapid vitrification Open
The aim of the study was to examine viability of cattle oocytes after cryopreservation. Oocytes after in vitro maturation (IVM) were vitrified in minimum volume on the nickel electron microscopy grids by ultra-rapid cooling technique. Afte…
View article: New gene markers involved in regulation of granulosa cells development and differentiation towards endodermal and epithelial tissues – a new insight into the stemness specificity of ovarian follicular cells
New gene markers involved in regulation of granulosa cells development and differentiation towards endodermal and epithelial tissues – a new insight into the stemness specificity of ovarian follicular cells Open
Maintaining of female fertility is strictly dependent on proper hormonal regulation. Granulosa cells (GCs) are components of ovarian follicles, and they are important in paracrine regulation of the ovary. Preovulatory follicle GCs are resp…
View article: Erratum to: The blood vessels development, morphogenesis and blood circulation are three ontologic groups highly up-regulated in porcine oocytes before in vitro maturation
Erratum to: The blood vessels development, morphogenesis and blood circulation are three ontologic groups highly up-regulated in porcine oocytes before in vitro maturation Open
Sciendo provides publishing services and solutions to academic and professional organizations and individual authors. We publish journals, books, conference proceedings and a variety of other publications.
View article: Erratum to: Expression changes in fatty acid metabolic process related genes in porcine oocytes during in vitro maturation
Erratum to: Expression changes in fatty acid metabolic process related genes in porcine oocytes during in vitro maturation Open
Sciendo provides publishing services and solutions to academic and professional organizations and individual authors. We publish journals, books, conference proceedings and a variety of other publications.
View article: Erratum to: Analysis of fructose and mannose – regulatory peptides signaling pathway in porcine epithelial oviductal cells (OECs) primary cultured long-term in vitro
Erratum to: Analysis of fructose and mannose – regulatory peptides signaling pathway in porcine epithelial oviductal cells (OECs) primary cultured long-term in vitro Open
Sciendo provides publishing services and solutions to academic and professional organizations and individual authors. We publish journals, books, conference proceedings and a variety of other publications.
View article: Challenges of stem cell application in research and clinical practice – an update
Challenges of stem cell application in research and clinical practice – an update Open
There are multiple possible applications of stem cells in medicine, from cell-based therapies for degenerative and dystrophic conditions, through novel approaches in cancer treatment, to in vitro organ printing. However, there are still se…
View article: Erratum to: Fatty acids related genes expression undergo substantial changes in porcine oviductal epithelial cells during long-term primary culture
Erratum to: Fatty acids related genes expression undergo substantial changes in porcine oviductal epithelial cells during long-term primary culture Open
Sciendo provides publishing services and solutions to academic and professional organizations and individual authors. We publish journals, books, conference proceedings and a variety of other publications.
View article: Endocrine disrupting chemicals and their detection in an IVF laboratory
Endocrine disrupting chemicals and their detection in an IVF laboratory Open
The endocrine disruptors are chemicals with the capacity to influence physiological processes in the organism, most often through hormonal control. They are present in the environment and in the products of daily use. They are often found …