Michał Lach
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View article: Autophagy Dysregulation in Crohn’s Disease and Colorectal Cancer—An Analysis of <em>BECN1</em>, <em>PINK1</em>, and <em>LAMP2 </em>Gene Expression
Autophagy Dysregulation in Crohn’s Disease and Colorectal Cancer—An Analysis of <em>BECN1</em>, <em>PINK1</em>, and <em>LAMP2 </em>Gene Expression Open
Crohn’s disease (CD) and colorectal cancer (CRC) are clinically distinct but pathogenetically related conditions in which significant abnormalities in autophagy are observed. The aim of the study was to evaluate the expression of three key…
View article: Neutralization of Toxic Malodorous Gases from Cattle Slurry
Neutralization of Toxic Malodorous Gases from Cattle Slurry Open
This study investigated the ability of bacterial strains to neutralize odorous substances from cattle slurry (CS). The research was performed to develop a microbial preparation for the deodorization of CS. Among the strains of bacteria (Ba…
View article: The Effect of Ionising Radiation on the Properties of Tumour-Derived Exosomes and Their Ability to Modify the Biology of Non-Irradiated Breast Cancer Cells—An In Vitro Study
The Effect of Ionising Radiation on the Properties of Tumour-Derived Exosomes and Their Ability to Modify the Biology of Non-Irradiated Breast Cancer Cells—An In Vitro Study Open
The vast majority of breast cancer patients require radiotherapy but some of them will develop local recurrences and potentially metastases in the future. Recent data show that exosomal cargo is essential in these processes. Thus, we inves…
View article: Photodynamic Treatment of Glioblastoma Plus Endothelial Cell Spheroid Models: Increased Proliferative and Migratory Aggressiveness of Surviving Tumor Cells Due to Inos/No Upregulation
Photodynamic Treatment of Glioblastoma Plus Endothelial Cell Spheroid Models: Increased Proliferative and Migratory Aggressiveness of Surviving Tumor Cells Due to Inos/No Upregulation Open
View article: Impact of Proton Irradiation Depending on Breast Cancer Subtype in Patient-Derived Cell Lines
Impact of Proton Irradiation Depending on Breast Cancer Subtype in Patient-Derived Cell Lines Open
Research on different types of ionizing radiation’s effects has been ongoing for years, revealing its efficacy in damaging cancer cells. Solid tumors comprise diverse cell types, each being able to respond differently to radiation. This st…
View article: Negative Effects of Occurrence of Mycotoxins in Animal Feed and Biological Methods of Their Detoxification: A Review
Negative Effects of Occurrence of Mycotoxins in Animal Feed and Biological Methods of Their Detoxification: A Review Open
Secondary metabolic products of molds, called mycotoxins, negatively affect animal health and production. They constitute a significant problem in veterinary and medical sciences, and their presence has been confirmed in feed all over the …
View article: The iPSC secretome is beneficial for in vitro propagation of primary osteoarthritic chondrocytes cell lines
The iPSC secretome is beneficial for in vitro propagation of primary osteoarthritic chondrocytes cell lines Open
Our preliminary results suggest a beneficial effect on maintaining chondrocyte biology during in vitro expansion.
View article: Conditioned Medium – Is it an Undervalued Lab Waste with the Potential for Osteoarthritis Management?
Conditioned Medium – Is it an Undervalued Lab Waste with the Potential for Osteoarthritis Management? Open
Background The approaches currently used in osteoarthritis (OA) are mainly short-term solutions with unsatisfactory outcomes. Cell-based therapies are still controversial (in terms of the sources of cells and the results) and require stric…
View article: MiRNAs from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression
MiRNAs from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression Open
Uveal melanoma (UM) is a rare type of malignancy that originates from melanocytes in the choroid, iris and the eye’s ciliary body. Biomarkers for early detection and progression of UM, especially the molecular traits governing the developm…
View article: The Induced Pluripotent Stem Cells in Articular Cartilage Regeneration and Disease Modelling: Are We Ready for Their Clinical Use?
The Induced Pluripotent Stem Cells in Articular Cartilage Regeneration and Disease Modelling: Are We Ready for Their Clinical Use? Open
The development of induced pluripotent stem cells has brought unlimited possibilities to the field of regenerative medicine. This could be ideal for treating osteoarthritis and other skeletal diseases, because the current procedures tend t…
View article: Multiplexed Immunobead-Based Cytokine Profiling in Patients with Ovarian Cancer
Multiplexed Immunobead-Based Cytokine Profiling in Patients with Ovarian Cancer Open
Due to late diagnosis, ovarian cancer is the most deadly gynecologic malignancy. Inflammation is one of the risk factors of ovarian cancer and the inflammatory response implicates all stages of tumorigenesis. The purpose of this study was …
View article: The Analysis of Inflammation-Related Proteins in a Cargo of Exosomes Derived from the Serum of Uveal Melanoma Patients Reveals Potential Biomarkers of Disease Progression
The Analysis of Inflammation-Related Proteins in a Cargo of Exosomes Derived from the Serum of Uveal Melanoma Patients Reveals Potential Biomarkers of Disease Progression Open
Background: Uveal melanoma (UM) is the most common intraocular tumour in adults with a poor prognosis and extremely high mortality rate due to the development of metastatic disease. However, despite relatively good knowledge about the hist…
View article: The involvement of small heat shock protein in chemoresistance in ovarian cancer - in vitro study
The involvement of small heat shock protein in chemoresistance in ovarian cancer - in vitro study Open
Ovarian cancer is the most deadly gynecologic malignancy worldwide. Although the primary response to chemotherapy is high, the majority of patients will develop resistance against applied treatment. In this study, we focused on resistance …
View article: DHMEQ enhances the cytotoxic effect of cisplatin and carboplatin in ovarian cancer cell lines.
DHMEQ enhances the cytotoxic effect of cisplatin and carboplatin in ovarian cancer cell lines. Open
Ovarian cancer (OvCa) is one of the most lethal gynaecological malignancies. It is diagnosed mostly in advanced stages. Due to a lack of appropriate early detection markers and non-ambiguous symptoms, the five-year survival rate is signifi…
View article: The effect of the combination of TGF-β1 and BMP2 with high-density pellet cell culture during chondrogenic differentiation of pluripotent stem cells.
The effect of the combination of TGF-β1 and BMP2 with high-density pellet cell culture during chondrogenic differentiation of pluripotent stem cells. Open
Introduction: The osteoarthritis is a serious threat for well-developed and ageing countries. Present techniques of treatment of damaged cartilage are not sufficient. Hence, new strategies should be developed. One of the potential sources …
View article: The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression
The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression Open
Uveal melanoma (UM) is the most common primary tumor of the eye diagnosed in adults, associated with a high risk of metastasis and thereby, poor prognosis. Among known risk factors for the development of metastatic disease is the loss of B…
View article: Overcoming Resistance to Platinum-Based Drugs in Ovarian Cancer by Salinomycin and Its Derivatives—An In Vitro Study
Overcoming Resistance to Platinum-Based Drugs in Ovarian Cancer by Salinomycin and Its Derivatives—An In Vitro Study Open
Polyether ionophore salinomycin (SAL) and its semi-synthetic derivatives are recognized as very promising anticancer drug candidates due to their activity against various types of cancer cells, including multidrug-resistant populations. Ov…
View article: Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions
Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions Open
The repair of damaged articular cartilage using currently available implantation techniques is not sufficient for the full recovery of patients. Pluripotent stem cells (iPSC)-based therapies could bring new perspectives in the treatment of…
View article: Fucoidan Exerts Anticancer Effects Against Head and Neck Squamous Cell Carcinoma In Vitro
Fucoidan Exerts Anticancer Effects Against Head and Neck Squamous Cell Carcinoma In Vitro Open
Fucoidans have been reported to exert anticancer effects with simultaneous low toxicity against healthy tissue. That correlation was observed in several cancer models, however, it has never been investigated in head and neck cancer before.…
View article: Effect of cellular mass on chondrogenic differentiation during embryoid body formation
Effect of cellular mass on chondrogenic differentiation during embryoid body formation Open
One approach to cell differentiation is to use the natural capacity of pluripotent stem cells to form three germ layers via embryoid bodies (EB). However, unification of this process during in vitro culture remains challenging and many mic…
View article: A feeder- and xeno-free human induced pluripotent stem cell line obtained from primary human dermal fibroblasts with epigenetic repression of reprogramming factors expression: GPCCi001-A
A feeder- and xeno-free human induced pluripotent stem cell line obtained from primary human dermal fibroblasts with epigenetic repression of reprogramming factors expression: GPCCi001-A Open
The primary human dermal fibroblasts (PHDFs) from breast cancer patient were obtained to generate the human induced pluripotent stem cell line GPCCi001-A via lentiviral transfection. Thus, a modified EF1a-hSTEMCCA-loxP with tetO operator w…
View article: Correlations between serum adipocytokine concentrations, disease stage, radiological status and total body fat content in the patients with primary knee osteoarthritis
Correlations between serum adipocytokine concentrations, disease stage, radiological status and total body fat content in the patients with primary knee osteoarthritis Open
It was found that in the obese patients with knee OA, increased percentage of total body fat and elevated serum leptin concentration might favour the advancement of clinical but not radiologically assessed changes in the joint structures, …
View article: Application of cell and biomaterial-based tissue engineering methods in the treatment of cartilage, menisci and ligament injuries
Application of cell and biomaterial-based tissue engineering methods in the treatment of cartilage, menisci and ligament injuries Open
Over 20 years ago it was realized that the traditional methods of the treatment of injuries to joint components: cartilage, menisci and ligaments, did not give satisfactory results and so there is a need of employing novel, more effective …
View article: The role of exosomes in tumor progression and metastasis (Review)
The role of exosomes in tumor progression and metastasis (Review) Open
Tumor cells have developed various mechanisms in defense against applied treatment, which prevent their total elimination from an organism. One of the underestimated mechanisms of defense is secretion of highly specialized double-membrane …
View article: Bioimaging: An Useful Tool to Monitor Differentiation of Human Embryonic Stem Cells into Chondrocytes
Bioimaging: An Useful Tool to Monitor Differentiation of Human Embryonic Stem Cells into Chondrocytes Open