Michelle DeWitt
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View article: Design of a potent interleukin-21 mimic for cancer immunotherapy
Design of a potent interleukin-21 mimic for cancer immunotherapy Open
Long-standing goals of cancer immunotherapy are to activate cytotoxic antitumor T cells across a range of affinities for tumor antigens while suppressing regulatory T cells. Computational protein design has enabled the precise tailoring of…
View article: Potent antitumor activity of a designed interleukin-21 mimic
Potent antitumor activity of a designed interleukin-21 mimic Open
Long-standing goals of cancer immunotherapy are to activate cytotoxic antitumor T cells across a broad range of affinities while dampening suppressive regulatory T (Treg) cell responses, but current approaches achieve these goals with limi…
View article: De novo design of luciferases using deep learning
De novo design of luciferases using deep learning Open
De novo enzyme design has sought to introduce active sites and substrate-binding pockets that are predicted to catalyse a reaction of interest into geometrically compatible native scaffolds 1,2 , but has been limited by a lack of suitable …
View article: <i>De novo</i>design of modular peptide binding proteins by superhelical matching
<i>De novo</i>design of modular peptide binding proteins by superhelical matching Open
General approaches for designing sequence-specific peptide binding proteins would have wide utility in proteomics and synthetic biology. Although considerable progress has been made in designing proteins which bind to other proteins, the g…
View article: De novo design of protein homodimers containing tunable symmetric protein pockets
De novo design of protein homodimers containing tunable symmetric protein pockets Open
Function follows form in biology, and the binding of small molecules requires proteins with pockets that match the shape of the ligand. For design of binding to symmetric ligands, protein homo-oligomers with matching symmetry are advantage…
View article: Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants
Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants Open
We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switch…
View article: Robust de novo design of protein binding proteins from target structural information alone
Robust de novo design of protein binding proteins from target structural information alone Open
The design of proteins that bind to a specific site on the surface of a target protein using no information other than the three-dimensional structure of the target remains an outstanding challenge. We describe a general solution to this p…
View article: Detection of antibodies neutralizing historical and emerging SARS-CoV-2 strains using a thermodynamically coupled de novo biosensor system
Detection of antibodies neutralizing historical and emerging SARS-CoV-2 strains using a thermodynamically coupled de novo biosensor system Open
With global vaccination efforts against SARS-CoV-2 underway, there is a need for rapid quantification methods for neutralizing antibodies elicited by vaccination and characterization of their strain dependence. Here, we describe a designed…
View article: Repurposing antimicrobial stewardship tools in the electronic medical record for the management of COVID-19 patients
Repurposing antimicrobial stewardship tools in the electronic medical record for the management of COVID-19 patients Open
During the COVID-19 pandemic, the antimicrobial stewardship module in our electronic medical record was reconfigured for the management of COVID-19 patients. This change allowed our subspecialist providers to review charts quickly to optim…
View article: Design and implementation of decision support for tobacco dependence treatment in an inpatient electronic medical record: a randomized trial
Design and implementation of decision support for tobacco dependence treatment in an inpatient electronic medical record: a randomized trial Open
www.ClinicalTrials.gov (NCT01691105).