Michiko Watanabe
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View article: A Simple and Fast Optical Clearing Method for Whole‐Mount Fluorescence In Situ Hybridization ( <scp>FISH</scp> ) Imaging
A Simple and Fast Optical Clearing Method for Whole‐Mount Fluorescence In Situ Hybridization ( <span>FISH</span> ) Imaging Open
We report a single‐step optical clearing method that is compatible with RNA fluorescence in situ hybridization (FISH) imaging. We previously demonstrated microscopy imaging with immunohistochemistry and genetic reporters using a technique …
View article: Identification and Characterization of Calcium Binding Protein, Spermatid Associated 1 (CABS1) in Selected Human Tissues and Fluids
Identification and Characterization of Calcium Binding Protein, Spermatid Associated 1 (CABS1) in Selected Human Tissues and Fluids Open
Calcium binding protein, spermatid associated 1 (CABS1) is a protein most widely studied in spermatogenesis. However, mRNA for CABS1 has been found in numerous tissues, albeit with little information about the protein. Previously, we ident…
View article: Segmentation of beating embryonic heart structures from 4-D OCT images using deep learning
Segmentation of beating embryonic heart structures from 4-D OCT images using deep learning Open
Optical coherence tomography (OCT) has been used to investigate heart development because of its capability to image both structure and function of beating embryonic hearts. Cardiac structure segmentation is a prerequisite for the quantifi…
View article: Segmentation of beating embryonic heart structures from 4-D OCT images using deep learning
Segmentation of beating embryonic heart structures from 4-D OCT images using deep learning Open
Optical coherence tomography (OCT) has been used to investigate heart development because of its capability to image both structure and function of beating embryonic hearts. Cardiac structure segmentation is a prerequisite for quantificati…
View article: Segmentation of beating embryonic heart structures from 4-D OCT images using deep learning
Segmentation of beating embryonic heart structures from 4-D OCT images using deep learning Open
Optical coherence tomography (OCT) has been used to investigate heart development because of its capability to image both structure and function of beating embryonic hearts. Cardiac structure segmentation is a prerequisite for quantificati…
View article: Issue Information
Issue Information Open
DEFECTS RESEARCH is published in 20 issues per year.Institutional subscription prices for 2023 are: Online Only: US $13,194 (US), US$13,194 (Rest of World), €8,513 (Europe), £6,737 (UK).Prices are exclusive of tax.Asia-Pacific GST,
View article: Emerging understanding of the effects of cannabis use during pregnancy
Emerging understanding of the effects of cannabis use during pregnancy Open
The negative effects of the use of marijuana (Cannabis sativa) on pregnancy outcomes is an area that urgently needs to be brought to the attention of the public and requires more and continuous research. The urgency is justified by the rap…
View article: Automated endocardial cushion segmentation and cellularization quantification in developing hearts using optical coherence tomography
Automated endocardial cushion segmentation and cellularization quantification in developing hearts using optical coherence tomography Open
Of all congenital heart defects (CHDs), anomalies in heart valves and septa are among the most common and contribute about fifty percent to the total burden of CHDs. Progenitors to heart valves and septa are endocardial cushions formed in …
View article: Differential immunostaining patterns of transient receptor potential (<scp>TRP</scp>) ion channels in the rat nodose ganglion
Differential immunostaining patterns of transient receptor potential (<span>TRP</span>) ion channels in the rat nodose ganglion Open
Vagal afferents regulate numerous physiological functions including arterial blood pressure, heart rate, breathing, and nociception. Cell bodies of vagal afferents reside in the inferior vagal (nodose) ganglia and their stimulation by vari…
View article: Assessment of coding-based frailty algorithms for long-term outcome prediction among older people in community settings: a cohort study from the Shizuoka Kokuho Database
Assessment of coding-based frailty algorithms for long-term outcome prediction among older people in community settings: a cohort study from the Shizuoka Kokuho Database Open
Objectives To assess the applicability of Electronic Frailty Index (eFI) and Hospital Frailty Risk Score (HFRS) algorithms to Japanese administrative claims data and to evaluate their association with long-term outcomes. Study Design and S…
View article: Development of an adenovirus-mediated reporter assay system to detect a low concentration of retinoic acid in MCF-7 cells
Development of an adenovirus-mediated reporter assay system to detect a low concentration of retinoic acid in MCF-7 cells Open
Retinoic acid, an active form of vitamin A, plays very important roles in mammalian embryogenesis. The concentration of retinoic acid is extremely low and strictly regulated by enzymes of cytochrome P450 (CYP) family, CYP26s (CYP26A1, CYP2…
View article: Prenatal ethanol exposure impairs the conduction delay at the atrioventricular junction in the looping heart
Prenatal ethanol exposure impairs the conduction delay at the atrioventricular junction in the looping heart Open
To the best of our knowledge, this is the first study investigating the impact of ethanol exposure on the early cardiac conduction system. Our results showed that ethanol-exposed embryos exhibited abnormally fast atrioventricular conductio…
View article: Abstracts
Abstracts Open
I am pleased to invite you to attend the 61st Annual Meeting of the Society for Birth Defects Research and Prevention, a fully online, virtual experience that will take place weekdays from June 24 to July 1, 2021, with three Education Cour…
View article: Introduction to the special focus on the development of the autonomic nervous system
Introduction to the special focus on the development of the autonomic nervous system Open
The autonomic nervous system (ANS) is a control system that, as the name implies, acts largely autonomously to regulate a variety of bodily functions, including breathing, digestion, heart rate and contraction, sweating, and pupillary, sex…
View article: Folic acid prevents functional and structural heart defects induced by prenatal ethanol exposure
Folic acid prevents functional and structural heart defects induced by prenatal ethanol exposure Open
State-of-the-art biophotonic tools captured blood flow and endocardial cushion volumes in tiny beating quail embryo hearts, an accessible model for studying four-chambered heart development. Both hemodynamic flow and endocardial cushion vo…
View article: Introduction for the special issue on “exercise during pregnancy”
Introduction for the special issue on “exercise during pregnancy” Open
Exercise is good for you!! That's the consensus with many studies and professional organizations such as the American Heart Association, the American College of Sports Medicine, and the U.S. Department of Health and Human Services strongly…
View article: Glutathione Protects the Developing Heart from Defects and Global DNA Hypomethylation Induced by Prenatal Alcohol Exposure
Glutathione Protects the Developing Heart from Defects and Global DNA Hypomethylation Induced by Prenatal Alcohol Exposure Open
Background Fetal alcohol spectrum disorder (FASD) is caused by prenatal alcohol exposure (PAE), the intake of ethanol (C 2 H 5 OH) during pregnancy. Features of FASD cover a range of structural and functional defects including congenital h…