Mike Boxem
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View article: The First of Us: Ophiocordyceps use a novel scramblase-binding peptide to manipulate zombie ants
The First of Us: Ophiocordyceps use a novel scramblase-binding peptide to manipulate zombie ants Open
Parasite-adaptive manipulation of behavior is a widespread natural phenomenon. While Ophiocordyceps zombie fungi are well-known for hijacking ant behavior to increase their fitness, functionally characterizing the biomolecules involved in …
View article: LGL-1 and the RhoGAP protein PAC-1 redundantly control polarization of the <i>C. elegans</i> embryonic epidermal epithelium
LGL-1 and the RhoGAP protein PAC-1 redundantly control polarization of the <i>C. elegans</i> embryonic epidermal epithelium Open
The formation of an apical–basal axis of polarity is essential for the organization and functioning of epithelial cells. Apical–basal polarity is established by cortical polarity proteins that define the apical, junctional, and basolateral…
View article: SUMO-mediated regulation of H3K4me3 reader SET-26 controls germline development in C. elegans
SUMO-mediated regulation of H3K4me3 reader SET-26 controls germline development in C. elegans Open
Sumoylation is a posttranslational modification essential for multiple cellular functions in eukaryotes. ULP-2 is a conserved SUMO protease required for embryonic development in Caenorhabditis elegans . Here, we revealed that ULP-2 control…
View article: SUMO-mediated regulation of a H3K4me3 reader controls germline development in<i>C. elegans</i>
SUMO-mediated regulation of a H3K4me3 reader controls germline development in<i>C. elegans</i> Open
ULP-2 is a conserved SUMO protease required for embryonic development in C. elegans . Here we revealed that ULP-2 controls germline development by regulating the PHD-SET domain protein, SET-26. Specifically, the ulp-2 mutant hermaphrodites…
View article: Intermediate filament network perturbation in the C. elegans intestine causes systemic dysfunctions
Intermediate filament network perturbation in the C. elegans intestine causes systemic dysfunctions Open
Intermediate filaments (IFs) are major components of the metazoan cytoskeleton. A long-standing debate concerns the question whether IF network organization only reflects or also determines cell and tissue function. Using Caenorhabditis el…
View article: Author response: Intermediate filament network perturbation in the C. elegans intestine causes systemic dysfunctions
Author response: Intermediate filament network perturbation in the C. elegans intestine causes systemic dysfunctions Open
Full text Figures and data Side by side Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Interme…
View article: The mIAA7 degron improves auxin-mediated degradation in <i>Caenorhabditis elegans</i>
The mIAA7 degron improves auxin-mediated degradation in <i>Caenorhabditis elegans</i> Open
Auxin-inducible degradation is a powerful tool for the targeted degradation of proteins with spatiotemporal control. One limitation of the auxin-inducible degradation system is that not all proteins are degraded efficiently. Here, we demon…
View article: Intermediate filament network perturbation in the <i>C. elegans</i> intestine causes systemic toxicity
Intermediate filament network perturbation in the <i>C. elegans</i> intestine causes systemic toxicity Open
Intermediate filaments (IFs) are major components of the metazoan cytoskeleton. A long-standing debate concerns the question whether IF network organization only reflects or also determines cell and tissue function. Using C. elegans , we h…
View article: The mIAA7 degron improves auxin-mediated degradation in <i>C. elegans</i>
The mIAA7 degron improves auxin-mediated degradation in <i>C. elegans</i> Open
Auxin-inducible degradation (AID) is a powerful tool for the targeted degradation of proteins with spatiotemporal control. One limitation of the AID system is that not all proteins are degraded efficiently. Here, we demonstrate that an alt…
View article: Identification and characterization of Crumbs polarity complex proteins in Caenorhabditis elegans
Identification and characterization of Crumbs polarity complex proteins in Caenorhabditis elegans Open
Crumbs proteins are evolutionarily conserved transmembrane proteins with essential roles in promoting the formation of the apical domain in epithelial cells. The short intracellular tail of Crumbs proteins are known to interact with severa…
View article: ERM-1 Phosphorylation and NRFL-1 Redundantly Control Lumen Formation in the C. elegans Intestine
ERM-1 Phosphorylation and NRFL-1 Redundantly Control Lumen Formation in the C. elegans Intestine Open
Reorganization of the plasma membrane and underlying actin cytoskeleton into specialized domains is essential for the functioning of most polarized cells in animals. Proteins of the ezrin-radixin-moesin (ERM) and Na + /H + exchanger 3 regu…
View article: Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells
Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells Open
The conserved adapter protein Scribble (Scrib) plays essential roles in a variety of cellular processes, including polarity establishment, proliferation, and directed cell migration. While the mechanisms through which Scrib promotes epithe…
View article: CeLINC, a fluorescence-based protein–protein interaction assay in <i>Caenorhabditis elegans</i>
CeLINC, a fluorescence-based protein–protein interaction assay in <i>Caenorhabditis elegans</i> Open
Interactions among proteins are fundamental for life and determining whether two particular proteins physically interact can be essential for fully understanding a protein’s function. We present Caenorhabditis elegans light-induced coclust…
View article: ERM-1 phosphorylation and NRFL-1 redundantly control lumen formation in the <i>C. elegans</i> intestine
ERM-1 phosphorylation and NRFL-1 redundantly control lumen formation in the <i>C. elegans</i> intestine Open
Reorganization of the plasma membrane and underlying actin cytoskeleton into specialized domains is essential for the functioning of most polarized cells in animals. Proteins of the ezrin-radixin-moesin (ERM) and Na + /H + exchanger 3 regu…
View article: Characterization of the composition and functioning of the Crumbs complex in <i>C. elegans</i>
Characterization of the composition and functioning of the Crumbs complex in <i>C. elegans</i> Open
The apical domain of epithelial cells can acquire a diverse array of morphologies and functions, which is critical for the function of epithelial tissues. The Crumbs proteins are evolutionary conserved transmembrane proteins with essential…
View article: CeLINC, a fluorescence-based protein-protein interaction assay in <i>C. elegans</i>
CeLINC, a fluorescence-based protein-protein interaction assay in <i>C. elegans</i> Open
Interactions among proteins are fundamental for life and determining whether two particular proteins physically interact can be essential for fully understanding a protein’s function. We present C. elegans light-induced co-clustering (CeLI…
View article: BBLN-1 is essential for intermediate filament organization and apical membrane morphology
BBLN-1 is essential for intermediate filament organization and apical membrane morphology Open
Epithelial tubes are essential components of metazoan organ systems that control the flow of fluids and the exchange of materials between body compartments and the outside environment. The size and shape of the central lumen confer importa…
View article: <i>Caenorhabditis elegans</i>LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells
<i>Caenorhabditis elegans</i>LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells Open
The conserved adapter protein Scribble (Scrib) plays essential roles in a variety of cellular processes, including polarity establishment, proliferation, and directed cell migration. While the mechanisms through which Scrib promotes epithe…
View article: Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules Open
The cortical polarity regulators PAR-6, PKC-3, and PAR-3 are essential for the polarization of a broad variety of cell types in multicellular animals. In C. elegans , the roles of the PAR proteins in embryonic development have been extensi…
View article: Author response: Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules
Author response: Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract The cortical polarity regulators PAR-6, PK…
View article: BBLN-1 is essential for intermediate filament organization and apical membrane morphology
BBLN-1 is essential for intermediate filament organization and apical membrane morphology Open
Epithelial tubes are essential components of metazoan organ systems that control the flow of fluids and the exchange of materials between body compartments and the outside environment. The size and shape of the central lumen confer importa…
View article: Epidermal PAR-6 and PKC-3 are essential for postembryonic development of<i>Caenorhabditis elegans</i>and control non-centrosomal microtubule organization
Epidermal PAR-6 and PKC-3 are essential for postembryonic development of<i>Caenorhabditis elegans</i>and control non-centrosomal microtubule organization Open
The cortical polarity regulators PAR-6, PKC-3 and PAR-3 are essential for the polarization of a broad variety of cell types in multicellular animals, from the first asymmetric division of the C. elegans zygote to apical–basal polarization …
View article: C-terminal phosphorylation modulates ERM-1 localization and dynamics to control cortical actin organization and support lumen formation during <i>Caenorhabditis</i> <i>elegans</i> development
C-terminal phosphorylation modulates ERM-1 localization and dynamics to control cortical actin organization and support lumen formation during <i>Caenorhabditis</i> <i>elegans</i> development Open
ERM proteins are conserved regulators of cortical membrane specialization that function as membrane-actin linkers and molecular hubs. The activity of ERM proteins requires a conformational switch from an inactive cytoplasmic form into an a…
View article: Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition
Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition Open
Here, to overcome many limitations accompanying current available methods to detect protein-protein interactions (PPIs), we develop a live cell method called Split Intein-Mediated Protein Ligation (SIMPL). In this approach, bait and prey p…
View article: Host interactors of effector proteins of the lettuce downy mildew Bremia lactucae obtained by yeast two-hybrid screening
Host interactors of effector proteins of the lettuce downy mildew Bremia lactucae obtained by yeast two-hybrid screening Open
Plant pathogenic bacteria, fungi and oomycetes secrete effector proteins to manipulate host cell processes to establish a successful infection. Over the last decade the genomes and transcriptomes of many agriculturally important plant path…
View article: <i>In vivo</i>control of the ezrin/radixin/moesin protein ERM-1 in<i>C. elegans</i>
<i>In vivo</i>control of the ezrin/radixin/moesin protein ERM-1 in<i>C. elegans</i> Open
ERM proteins are conserved regulators of cortical membrane specialization, that function as membrane–actin linkers and molecular hubs. Activity of ERM proteins requires a conformational switch from an inactive cytoplasmic form into an acti…
View article: Host targets of effectors of the lettuce downy mildew <i>Bremia lactucae</i> from cDNA-based yeast two-hybrid screening
Host targets of effectors of the lettuce downy mildew <i>Bremia lactucae</i> from cDNA-based yeast two-hybrid screening Open
Plant pathogenic bacteria, fungi and oomycetes secrete effector proteins to manipulate host cell processes to establish a successful infection. Over the last decade the genomes and transcriptomes of many agriculturally important plant path…
View article: Functional Dissection of C. elegans bZip-Protein CEBP-1 Reveals Novel Structural Motifs Required for Axon Regeneration and Nuclear Import
Functional Dissection of C. elegans bZip-Protein CEBP-1 Reveals Novel Structural Motifs Required for Axon Regeneration and Nuclear Import Open
The basic leucine-zipper (bZIP) domain transcription factors CCAAT/enhancer-binding proteins (C/EBP) have a variety of roles in cell proliferation, differentiation, and stress response. In the nervous system, several isoforms of C/EBP func…