Mike Manefield
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View article: Ascorbic acid modulates the structure of the Pseudomonas aeruginosa virulence factor pyocyanin and ascorbic acid-furanone-30 combination facilitate biofilm disruption
Ascorbic acid modulates the structure of the Pseudomonas aeruginosa virulence factor pyocyanin and ascorbic acid-furanone-30 combination facilitate biofilm disruption Open
The production of pyocyanin by Pseudomonas aeruginosa increases its virulence, fitness and biofilm formation. Pyocyanin is also a redox molecule and we hypothesize that ascorbic acid being an antioxidant will interact with pyocyanin. The m…
View article: Microbiology in sustainable remediation of contaminated sites
Microbiology in sustainable remediation of contaminated sites Open
The release of chemicals that have negative human or environmental health impacts has been rife around the world for a century. Approaches to contaminated site remediation have evolved over this time to address environmental contamination.…
View article: Microbiologically influenced corrosion of cable bolts in underground coal mines: The effect of Acidithiobacillus ferrooxidans
Microbiologically influenced corrosion of cable bolts in underground coal mines: The effect of Acidithiobacillus ferrooxidans Open
View article: Constraining source attribution of methane in an alluvial aquifer with multiple recharge pathways
Constraining source attribution of methane in an alluvial aquifer with multiple recharge pathways Open
Identifying the source of methane (CH4) in groundwater is often complicated due to various production, degradation and migration pathways, particularly in settings where there are multiple groundwater recharge pathways. This stu…
View article: Microbiologically induced cable bolt corrosion in underground coal mines
Microbiologically induced cable bolt corrosion in underground coal mines Open
Corrosion failure is one of the serious concerns with most metal products.In the mining industry, corrosion failure of cable bolts may threaten the safety of operations and cause significant financial losses.In recent years, many cable bol…
View article: Dependency of DNA extraction efficiency on cell concentration confounds molecular quantification of microorganisms in groundwater
Dependency of DNA extraction efficiency on cell concentration confounds molecular quantification of microorganisms in groundwater Open
Quantification of microbes in water systems is essential to industrial practices ranging from drinking water and wastewater treatment to groundwater remediation. While quantification using DNA-based molecular methods is precise, the accura…
View article: Glutathione Enhances Antibiotic Efficiency and Effectiveness of DNase I in Disrupting Pseudomonas aeruginosa Biofilms While Also Inhibiting Pyocyanin Activity, Thus Facilitating Restoration of Cell Enzymatic Activity, Confluence and Viability
Glutathione Enhances Antibiotic Efficiency and Effectiveness of DNase I in Disrupting Pseudomonas aeruginosa Biofilms While Also Inhibiting Pyocyanin Activity, Thus Facilitating Restoration of Cell Enzymatic Activity, Confluence and Viability Open
© 2017 Das, Simone, Ibugo, Witting, Manefield and Manos. Pyocyanin secreted by Pseudomonas aeruginosa is a virulence factor that damages epithelial cells during infection through the action of reactive oxygen species, however, little is kn…
View article: Design and synthesis of short amphiphilic cationic peptidomimetics based on biphenyl backbone as antibacterial agents
Design and synthesis of short amphiphilic cationic peptidomimetics based on biphenyl backbone as antibacterial agents Open
View article: Co-occurrence of genes for aerobic and anaerobic biodegradation of dichloroethane in organochlorine-contaminated groundwater
Co-occurrence of genes for aerobic and anaerobic biodegradation of dichloroethane in organochlorine-contaminated groundwater Open
1,2-Dichloroethane (DCA) is a problematic groundwater pollutant. Factors influencing the distribution and activities of DCA-degrading bacteria are not well understood, which has hampered their application for bioremediation. Here, we used …
View article: Nutrient and acetate amendment leads to acetoclastic methane production and microbial community change in a non‐producing Australian coal well
Nutrient and acetate amendment leads to acetoclastic methane production and microbial community change in a non‐producing Australian coal well Open
Summary Coal mining is responsible for 11% of total anthropogenic methane emission thereby contributing considerably to climate change. Attempts to harvest coalbed methane for energy production are challenged by relatively low methane conc…
View article: A bacterial chloroform reductive dehalogenase: purification and biochemical characterization
A bacterial chloroform reductive dehalogenase: purification and biochemical characterization Open
Summary We report herein the purification of a chloroform ( CF )‐reducing enzyme, TmrA, from the membrane fraction of a strict anaerobe Dehalobacter sp. strain UNSWDHB to apparent homogeneity with an approximate 23‐fold increase in relativ…
View article: Isolation and Characterization of Dehalobacter sp. Strain TeCB1 Including Identification of TcbA: A Novel Tetra- and Trichlorobenzene Reductive Dehalogenase
Isolation and Characterization of Dehalobacter sp. Strain TeCB1 Including Identification of TcbA: A Novel Tetra- and Trichlorobenzene Reductive Dehalogenase Open
Dehalobacter sp. strain TeCB1 was isolated from groundwater near Sydney, Australia, that is polluted with a range of organochlorines. The isolated strain is able to grow by reductive dechlorination of 1,2,4,5-tetrachlorobenzene to 1…
View article: Investigation of the microbial communities colonizing prepainted steel used for roofing and walling
Investigation of the microbial communities colonizing prepainted steel used for roofing and walling Open
© 2016 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. Microbial colonization of prepainted steel, commonly used in roofing applications, impacts their aesthetics, durability, and functionality. Understanding the relevant…
View article: Genome Sequence of <i>Dehalobacter</i> sp. Strain TeCB1, Able To Respire Chlorinated Benzenes
Genome Sequence of <i>Dehalobacter</i> sp. Strain TeCB1, Able To Respire Chlorinated Benzenes Open
Dehalobacter sp. strain TeCB1 was isolated from groundwater contaminated with a mixture of organohalides and is able to respire 1,2,4,5-tetrachlorobenzene and 1,2,4-trichlorobenzene. Here, we report its 3.13-Mb draft genome sequence.
View article: Biogeochemical constraints on the origin of methane in an alluvial aquifer: evidence for the upward migration of methane from underlying coal measures
Biogeochemical constraints on the origin of methane in an alluvial aquifer: evidence for the upward migration of methane from underlying coal measures Open
Geochemical and microbiological indicators of methane (CH4) production, oxidation and migration processes in groundwater are important to understand when attributing sources of gas. The processes controlling the natural occurrence of CH4 i…
View article: Investigation of the microbial communities colonizing prepainted steel used for roofing and walling
Investigation of the microbial communities colonizing prepainted steel used for roofing and walling Open
Microbial colonization of prepainted steel, commonly used in roofing applications, impacts their aesthetics, durability, and functionality. Understanding the relevant organisms and the mechanisms by which colonization occurs would provide …
View article: Biogeochemical constraints on the origin of methane in an alluvial aquifer: evidence for the upward migration of methane from a coal seam
Biogeochemical constraints on the origin of methane in an alluvial aquifer: evidence for the upward migration of methane from a coal seam Open
Geochemical and microbiological indicators of methane (CH4) production, oxidation and migration processes in groundwater are important to understand when attributing sources of gas. The processes controlling the natural occurrence of CH4 i…
View article: Supplementary material to "Biogeochemical constraints on the origin of methane in an alluvial aquifer: evidence for the upward migration of methane from a coal seam"
Supplementary material to "Biogeochemical constraints on the origin of methane in an alluvial aquifer: evidence for the upward migration of methane from a coal seam" Open
View article: N-Acetylglucosamine Inhibits LuxR, LasR and CviR Based Quorum Sensing Regulated Gene Expression Levels
N-Acetylglucosamine Inhibits LuxR, LasR and CviR Based Quorum Sensing Regulated Gene Expression Levels Open
N-acetyl glucosamine, the monomer of chitin, is an abundant source of carbon and nitrogen in nature as it is the main component and breakdown product of many structural polymers. Some bacteria use N-acyl-L-homoserine lactone (AHL) mediated…
View article: High-rate, High Temperature Acetotrophic Methanogenesis Governed by a Three Population Consortium in Anaerobic Bioreactors
High-rate, High Temperature Acetotrophic Methanogenesis Governed by a Three Population Consortium in Anaerobic Bioreactors Open
A combination of acetate oxidation and acetoclastic methanogenesis has been previously identified to enable high-rate methanogenesis at high temperatures (55 to 65°C), but this capability had not been linked to any key organisms. This stud…
View article: Serratia Secondary Metabolite Prodigiosin Inhibits Pseudomonas aeruginosa Biofilm Development by Producing Reactive Oxygen Species that Damage Biological Molecules
Serratia Secondary Metabolite Prodigiosin Inhibits Pseudomonas aeruginosa Biofilm Development by Producing Reactive Oxygen Species that Damage Biological Molecules Open
Prodigiosin is a heterocyclic bacterial secondary metabolite belonging to the class of tripyrrole compounds, synthesized by various types of bacteria including Serratia species. Prodigiosin has been the subject of intense research over the…
View article: Application of stable isotope tools for evaluating natural and stimulated biodegradation of organic pollutants in field studies
Application of stable isotope tools for evaluating natural and stimulated biodegradation of organic pollutants in field studies Open
View article: Glutathione-Disrupted Biofilms of Clinical Pseudomonas aeruginosa Strains Exhibit an Enhanced Antibiotic Effect and a Novel Biofilm Transcriptome
Glutathione-Disrupted Biofilms of Clinical Pseudomonas aeruginosa Strains Exhibit an Enhanced Antibiotic Effect and a Novel Biofilm Transcriptome Open
Pseudomonas aeruginosa infections result in high morbidity and mortality rates for individuals with cystic fibrosis (CF), with premature death often occurring. These infections are complicated by the formation of biofilms in the sputum. An…
View article: Isolation and characterization of <i>Dehalobacter sp.</i> strain UNSWDHB capable of chloroform and chlorinated ethane respiration
Isolation and characterization of <i>Dehalobacter sp.</i> strain UNSWDHB capable of chloroform and chlorinated ethane respiration Open
Summary Dehalobacter sp. strain UNSWDHB can dechlorinate up to 4 mM trichloromethane at a rate of 0.1 mM per day to dichloromethane and 1,1,2‐trichloroethane (1 mM, 0.1 mM per day) with the unprecedented product profile of 1,2‐dichloroetha…
View article: Synthesis and biological evaluation of N-naphthoyl-phenylglyoxamide-based small molecular antimicrobial peptide mimics as novel antimicrobial agents and biofilm inhibitors
Synthesis and biological evaluation of N-naphthoyl-phenylglyoxamide-based small molecular antimicrobial peptide mimics as novel antimicrobial agents and biofilm inhibitors Open
The synthesis of novel glyoxamide-based antimicrobial peptide mimics is described.
View article: Novel phenazine crystals enable direct electron transfer to methanogens in anaerobic digestion by redox potential modulation
Novel phenazine crystals enable direct electron transfer to methanogens in anaerobic digestion by redox potential modulation Open
Phenazine crystals enhance methanogenesis by electron delivery to respiratory heterodisulfide reductase enzyme.
View article: Phenazine virulence factor binding to extracellular DNA is important for Pseudomonas aeruginosa biofilm formation
Phenazine virulence factor binding to extracellular DNA is important for Pseudomonas aeruginosa biofilm formation Open
View article: The role of lipids in activated sludge floc formation
The role of lipids in activated sludge floc formation Open
Activated sludge is widely used to treat municipal and industrial wastewater globally and the formation of activated sludge flocculates (flocs) underpins the ability to separate sludge from treated water. Despite the importance of activate…
View article: The role of lipids in activated sludge floc formation
The role of lipids in activated sludge floc formation Open
Activated sludge is widely used to treat municipal and industrial wastewater globally and the formation of activated sludge flocculates (flocs) underpins the ability to separate sludge from treated water. Despite the importance of activate…
View article: CCDC 1020368: Experimental Crystal Structure Determination
CCDC 1020368: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …