Michael P. Washburn
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View article: Integrated multi-omic analysis reveals novel subtype-specific regulatory interactions in pediatric B-cell acute lymphoblastic leukemia
Integrated multi-omic analysis reveals novel subtype-specific regulatory interactions in pediatric B-cell acute lymphoblastic leukemia Open
Molecular subtyping of pediatric B-cell acute lymphoblastic leukemia (B-ALL) has improved patient outcomes through stratification and selection of targeted therapies. Despite extensive genomic and transcriptomic profiling of this cancer, f…
View article: Integrative Structural Modeling of Intrinsically Disordered Regions in a Human HDAC2 Chromatin Remodeling Complex
Integrative Structural Modeling of Intrinsically Disordered Regions in a Human HDAC2 Chromatin Remodeling Complex Open
Intrinsically disordered regions (IDRs) and intrinsically disordered proteins (IDPs) play pivotal roles in cellular signaling, molecular recognition, and the regulation of various biological processes. These flexible and conformationally d…
View article: Cotranslational assembly confers specificity for in vivo target heterodimerization of paralogous H2B-like TAF12 proteins in the human fungal pathogen<i>Candida albicans</i>
Cotranslational assembly confers specificity for in vivo target heterodimerization of paralogous H2B-like TAF12 proteins in the human fungal pathogen<i>Candida albicans</i> Open
The fidelity of assembly of multiprotein complexes is essential for the formation of stable and functional protein complexes that are critical for cell growth and survival. In this context, TBP-associated factor (TAF) subunits maintain tig…
View article: TRIM16 mediates secretory autophagy in head and neck cancer-associated fibroblasts
TRIM16 mediates secretory autophagy in head and neck cancer-associated fibroblasts Open
Improving treatment options for head and neck squamous cell carcinoma (HNSCC) requires a deeper understanding of the tumor microenvironment, particularly cancer-associated fibroblasts (CAFs). We previously reported that HNSCC-derived FGF2/…
View article: <i>BRCA1</i> influences whole body metabolism in humanized mice
<i>BRCA1</i> influences whole body metabolism in humanized mice Open
This is the first in vivo evidence demonstrating the complex effects of BRCA1 expression in whole body metabolism.
View article: Human skeletal muscle mitochondrial pathways are impacted by a neuropathologic diagnosis of Alzheimer's disease
Human skeletal muscle mitochondrial pathways are impacted by a neuropathologic diagnosis of Alzheimer's disease Open
Alzheimer's disease (AD) is associated with reduced lean mass and impaired skeletal muscle mitochondrial and motor function. Although primary mitochondrial defects in AD may underlie these findings, molecular alterations in AD have not bee…
View article: 535 Multiomic analysis of KMT2A-r pediatric acute myeloid leukemia
535 Multiomic analysis of KMT2A-r pediatric acute myeloid leukemia Open
Objectives/Goals: Acute myeloid leukemia (AML) is the second most common leukemia among pediatric populations. Approximately 15% of pediatric AML cases have KMT2A gene rearrangements (KMT2A-r), which confers a worse prognosis. Our goal is …
View article: Differential Remodeling of the BRCA1 Interactome by a Unique Minimal Clinical Truncation Mutation
Differential Remodeling of the BRCA1 Interactome by a Unique Minimal Clinical Truncation Mutation Open
BRCA1, a critical tumor suppressor gene, plays an essential role in maintaining genomic stability through its involvement in DNA double-strand break repair, particularly via homologous recombination. Loss or impairment of BRCA1 function di…
View article: Analysis of FAIMS for the Study of Affinity-Purified Protein Complexes Using the Orbitrap Ascend Tribrid Mass Spectrometer
Analysis of FAIMS for the Study of Affinity-Purified Protein Complexes Using the Orbitrap Ascend Tribrid Mass Spectrometer Open
In this study, we analyzed the combination of affinity purification mass spectrometry (AP-MS) with high-field asymmetric waveform ion mobility spectrometry (FAIMS), integrated between nanoLC-MS and an Orbitrap Ascend Tribrid Mass Spectrome…
View article: Proteomic Characterization of Pediatric Acute Lymphoblastic Leukemias Sheds Light on Subtype-Specific Molecular Mechanisms
Proteomic Characterization of Pediatric Acute Lymphoblastic Leukemias Sheds Light on Subtype-Specific Molecular Mechanisms Open
Acute lymphoblastic leukemia (ALL) accounts for approximately one third of cancers diagnosed in children every year. Molecular subtyping has significantly improved patient stratification and treatment selection for pediatric leukemia patie…
View article: DYRK1A interacts with the tuberous sclerosis complex and promotes mTORC1 activity
DYRK1A interacts with the tuberous sclerosis complex and promotes mTORC1 activity Open
DYRK1A, a ubiquitously expressed kinase , is linked to the dominant intellectual developmental disorder, microcephaly, and Down syndrome in humans. It regulates numerous cellular processes such as cell cycle, vesicle trafficking, and micro…
View article: DYRK1A Interacts with the Tuberous Sclerosis Complex and Promotes mTORC1 Activity
DYRK1A Interacts with the Tuberous Sclerosis Complex and Promotes mTORC1 Activity Open
DYRK1A, a ubiquitously expressed kinase, is linked to the dominant intellectual developmental disorder, microcephaly and Down syndrome in humans. It regulates numerous cellular processes such as cell cycle, vesicle trafficking and microtub…
View article: Author response: DYRK1A Interacts with the Tuberous Sclerosis Complex and Promotes mTORC1 Activity
Author response: DYRK1A Interacts with the Tuberous Sclerosis Complex and Promotes mTORC1 Activity Open
DYRK1A, a ubiquitously expressed kinase, is linked to the dominant intellectual developmental disorder, microcephaly and Down syndrome in humans. It regulates numerous cellular processes such as cell cycle, vesicle trafficking and microtub…
View article: An integrated structural model of the DNA damage-responsive H3K4me3 binding WDR76:SPIN1 complex with the nucleosome
An integrated structural model of the DNA damage-responsive H3K4me3 binding WDR76:SPIN1 complex with the nucleosome Open
Serial capture affinity purification (SCAP) is a powerful method to isolate a specific protein complex. When combined with cross-linking mass spectrometry and computational approaches, one can build an integrated structural model of the is…
View article: The Orphan G Protein-Coupled Receptor GPR52 is a Novel Regulator of Breast Cancer Multicellular Organization
The Orphan G Protein-Coupled Receptor GPR52 is a Novel Regulator of Breast Cancer Multicellular Organization Open
Statement of Significance We showed that loss of the orphan G protein-coupled receptor GPR52 in human breast cell lines leads to increased cell clustering, hybrid/partial EMT, and increased tumor burden in zebrafish. Background G protein-c…
View article: Proteomic profiling of bronchoalveolar lavage fluid uncovers protein clusters linked to survival in idiopathic forms of interstitial lung disease
Proteomic profiling of bronchoalveolar lavage fluid uncovers protein clusters linked to survival in idiopathic forms of interstitial lung disease Open
Background Idiopathic interstitial pneumonias (IIPs), such as idiopathic pulmonary fibrosis and interstitial pneumonia with autoimmune features, present diagnostic and therapeutic challenges due to their heterogeneous nature. This study ai…
View article: Proteomic profiling of bronchoalveolar lavage fluid uncovers protein clusters linked to survival in idiopathic forms of interstitial lung disease
Proteomic profiling of bronchoalveolar lavage fluid uncovers protein clusters linked to survival in idiopathic forms of interstitial lung disease Open
Background Idiopathic interstitial pneumonias (IIPs) such as idiopathic pulmonary fibrosis (IPF) and interstitial pneumonia with autoimmune features (IPAF), present diagnostic and therapeutic challenges due to their heterogeneous nature. T…
View article: DCLK1-Mediated Regulation of Invadopodia Dynamics and Matrix Metalloproteinase Trafficking Drives Invasive Progression in Head and Neck Squamous Cell Carcinoma
DCLK1-Mediated Regulation of Invadopodia Dynamics and Matrix Metalloproteinase Trafficking Drives Invasive Progression in Head and Neck Squamous Cell Carcinoma Open
Head and neck squamous cell carcinoma (HNSCC) is a major health concern due to its high mortality from poor treatment responses and locoregional tumor invasion into life sustaining structures in the head and neck. A deeper comprehension of…
View article: Distinct regions within SAP25 recruit O-linked glycosylation, DNA demethylation, and ubiquitin ligase and hydrolase activities to the Sin3/HDAC complex
Distinct regions within SAP25 recruit O-linked glycosylation, DNA demethylation, and ubiquitin ligase and hydrolase activities to the Sin3/HDAC complex Open
Summary Epigenetic control of gene expression is crucial for maintaining gene regulation. Sin3 is an evolutionarily conserved repressor protein complex mainly associated with histone deacetylase (HDAC) activity. A large number of proteins …
View article: Neutrophil extracellular traps linked to idiopathic pulmonary fibrosis severity and survival
Neutrophil extracellular traps linked to idiopathic pulmonary fibrosis severity and survival Open
Background Idiopathic pulmonary fibrosis (IPF) leads to progressive loss of lung function and mortality. Understanding mechanisms and markers of lung injury in IPF is paramount to improving outcomes for these patients. Despite the lack of …
View article: Beyond the Sin3/HDAC Complex: FAM60A emerges as a regulator of RNA Splicing
Beyond the Sin3/HDAC Complex: FAM60A emerges as a regulator of RNA Splicing Open
FAM60A, traditionally linked to chromatin remodeling within the Sin3/HDAC complex, has emerged as a critical regulator in RNA splicing. Employing an integrative approach that combines immunological assays, CRISPR/Cas9 technology, comprehen…
View article: The antiproliferative effect of FGF2 in K-Ras-driven tumor cells involves modulation of rRNA and the nucleolus
The antiproliferative effect of FGF2 in K-Ras-driven tumor cells involves modulation of rRNA and the nucleolus Open
The nucleolus is sensitive to stress and can orchestrate a chain of cellular events in response to stress signals. Despite being a growth factor, FGF2 has antiproliferative and tumor-suppressive functions in some cellular contexts. In this…
View article: Small Intestinal Polyp Burden in Pediatric Peutz–Jeghers Syndrome Assessed through Capsule Endoscopy: A Longitudinal Study
Small Intestinal Polyp Burden in Pediatric Peutz–Jeghers Syndrome Assessed through Capsule Endoscopy: A Longitudinal Study Open
The management of pediatric Peutz–Jeghers Syndrome (PJS) focuses on the prevention of intussusception complicating small intestinal (SI) polyposis. This hinges on the accurate appraisal of the polyp burden to tailor therapeutic interventio…
View article: Personalized Cancer Monitoring Assay for the Detection of ctDNA in Patients with Solid Tumors
Personalized Cancer Monitoring Assay for the Detection of ctDNA in Patients with Solid Tumors Open
The Invitae Personalized Cancer Monitoring assay, featuring a flexible patient-specific panel design with 18-50 variants, demonstrated high sensitivity and specificity for detecting ctDNA at variant allele frequencies as low as 0.008%. Thi…
View article: Small Intestinal Polyp Burden in Pediatric Peutz-Jeghers Syndrome
Small Intestinal Polyp Burden in Pediatric Peutz-Jeghers Syndrome Open
The authors have requested that this preprint be removed from Research Square.
View article: DYRK1A interacts with the tuberous sclerosis complex and promotes mTORC1 activity
DYRK1A interacts with the tuberous sclerosis complex and promotes mTORC1 activity Open
DYRK1A, a ubiquitously expressed kinase , is linked to the dominant intellectual developmental disorder, microcephaly, and Down syndrome in humans. It regulates numerous cellular processes such as cell cycle, vesicle trafficking, and micro…