Milan Polakovič
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View article: Galactooligosaccharide Production Using Immobilized Aspergillus oryzae β-Galactosidase, Part I: Characterization and Influence of Reaction Conditions
Galactooligosaccharide Production Using Immobilized Aspergillus oryzae β-Galactosidase, Part I: Characterization and Influence of Reaction Conditions Open
The enzymatic production of prebiotic galactooligosaccharides (GOS), functional food ingredients with established health benefits, remains an active research area driven by a rising global demand for GOS. These oligosaccharides are synthes…
View article: Protein Adsorption on a Multimodal Cation Exchanger: Effect of pH, Salt Type and Concentration, and Elution Conditions
Protein Adsorption on a Multimodal Cation Exchanger: Effect of pH, Salt Type and Concentration, and Elution Conditions Open
This study investigates key factors affecting the adsorption behavior of proteins on the multimodal chromatographic adsorbent Capto MMC, aiming to enhance selective protein separation strategies. Batch equilibrium experiments were conducte…
View article: β-Galactosidase-Catalyzed Transglycosylation of Tyrosol: Substrates and Deep Eutectic Solvents Affecting Activity and Stability
β-Galactosidase-Catalyzed Transglycosylation of Tyrosol: Substrates and Deep Eutectic Solvents Affecting Activity and Stability Open
β-Galactosidase, a glycoside hydrolase enzyme, also possesses glycosyl transferase activity and can glycosylate various aglycones, including tyrosol, a phenylethanoid with antioxidant and health-promoting effects. This study examines the e…
View article: Antibody Aggregate Removal by Multimodal Chromatography
Antibody Aggregate Removal by Multimodal Chromatography Open
The growing demand for therapeutic monoclonal antibodies (mAbs) has heightened the need for efficient and scalable purification strategies. A major challenge in downstream processing is the removal of antibody aggregates, which can comprom…
View article: Optimization of growth and induction conditions for the production of recombinant whole cell cyclohexanone monooxygenase in Escherichia coli
Optimization of growth and induction conditions for the production of recombinant whole cell cyclohexanone monooxygenase in Escherichia coli Open
View article: A fluorescent dye assay for antibody aggregates and its application in aggregate stability evaluation
A fluorescent dye assay for antibody aggregates and its application in aggregate stability evaluation Open
Aggregation of proteins naturally occurs during different stages of cultivation and downstream processes and is often associated with quality reduction in the final product. Therefore, determining the presence of aggregates is important. I…
View article: Yeast β‒fructosidase-catalyzed synthesis of tyrosol fructoside in mono- and biphasic organic–aqueous systems
Yeast β‒fructosidase-catalyzed synthesis of tyrosol fructoside in mono- and biphasic organic–aqueous systems Open
Tyrosol and its glycosides offer cell protection from oxidative stress and various health benefits. In this study, Saccharomyces cerevisiae β ‒fructosidase potential for synthesizing tyrosol fructoside in mono- and biphasic solvent systems…
View article: Catalytic Stability of S-1-(4-Hydroxyphenyl)-Ethanol Dehydrogenase from Aromatoleum aromaticum
Catalytic Stability of S-1-(4-Hydroxyphenyl)-Ethanol Dehydrogenase from Aromatoleum aromaticum Open
Derived from the denitrifying bacterium Aromatoleum aromaticum EbN1 (Azoarcus sp.), the enzyme S-1-(4-hydroxyphenyl)-ethanol dehydrogenase (S-HPED) belongs to the short-chain dehydrogenase/reductase family. Using research techniques like U…
View article: Separation of (2E,4E)-deca-2,4-dienal from a Biocatalytic Reaction Mixture Using Hydrophobic Adsorbents
Separation of (2E,4E)-deca-2,4-dienal from a Biocatalytic Reaction Mixture Using Hydrophobic Adsorbents Open
Biocatalytic oxidation of a fatty acid preparation by enzymes released from a plant extract provided (2E,4E)-deca-2,4-dienal (2,4-DDAL), a valuable natural aroma. The post-reaction mixture contained several other compounds, from which 2,4-…
View article: Production of Fructooligosaccharides Using a Commercial Heterologously Expressed Aspergillus sp. Fructosyltransferase
Production of Fructooligosaccharides Using a Commercial Heterologously Expressed Aspergillus sp. Fructosyltransferase Open
The catalytic properties of Seqenzym® FT, a fungal fructosyltransferase heterologously expressed in yeasts, were investigated at a temperature of 55 °C and pH 5.5. The initial rate measurements showed that the transfructosylation rate was …
View article: Investigation of process stability of a whole-cell biocatalyst with Baeyer–Villiger monooxygenase activity in continuous bioreactors
Investigation of process stability of a whole-cell biocatalyst with Baeyer–Villiger monooxygenase activity in continuous bioreactors Open
The stability of a whole-cell biocatalyst based on genetically modified Escherichia coli cells harboring cyclohexanone monooxygenase was investigated in a free and immobilized form in continuous bioreactors. The biotransformation of a bicy…
View article: Adsorption Performance of a Multimodal Anion-Exchange Chromatography Membrane: Effect of Liquid Phase Composition and Separation Mode
Adsorption Performance of a Multimodal Anion-Exchange Chromatography Membrane: Effect of Liquid Phase Composition and Separation Mode Open
Membrane chromatography is a modern, high-throughput separation method that finds important applications in therapeutic protein purification. Multimodal, salt-tolerant membranes are the most recent innovation in chromatographic membrane ad…
View article: Apiin-induction of <i>β</i>-apiosidase production by <i>Aspergillus</i> sp. strains
Apiin-induction of <i>β</i>-apiosidase production by <i>Aspergillus</i> sp. strains Open
β -Apiosidase is a rare glycosidase applied in winemaking for flavour enhancement. This enzyme is involved in the release of volatile terpenes by hydrolysis of their odourless glycosidic precursors. It is found as a minor component in comm…
View article: Assessing teaching effectiveness in higher education
Assessing teaching effectiveness in higher education Open
Recently, a robust tool for assessment of different teaching methodologies in engineering education has been developed by the consortium of the iTeach project (www.iteach-chemeng.eu). The tool was initially tested for its applicability in …
View article: Corrigendum to: Thermal inactivation kinetics of <i>Aspergillus oryzae β</i>-galactosidase in concentrated lactose solution
Corrigendum to: Thermal inactivation kinetics of <i>Aspergillus oryzae β</i>-galactosidase in concentrated lactose solution Open
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View article: Recombinant human erythropoietin separation using a cation-exchange multimodal adsorbent
Recombinant human erythropoietin separation using a cation-exchange multimodal adsorbent Open
This work deals with the capture of human recombinant erythropoietin (rhEPO) from a mixture of proteins in a concentrated postcultivation supernatant. Cation-exchange multimodal adsorbent Capto MMC ImpRes was selected as potential chromato…
View article: Design of operational temperature for immobilized glucose isomerise using an accelerated inactivation method
Design of operational temperature for immobilized glucose isomerise using an accelerated inactivation method Open
Thermal inactivation of immobilized glucose isomerase in a concentrated glucose solution was investigated in the batch mode and temperature range of 83–95 °C, which is substantially higher than the temperature used in the industrial produc…
View article: Thermal inactivation kinetics of <i>Aspergillus oryzae β</i>-galactosidase in concentrated lactose solution
Thermal inactivation kinetics of <i>Aspergillus oryzae β</i>-galactosidase in concentrated lactose solution Open
Thermal inactivation of a commercial β -galactosidase from Aspergillus oryzae in a 300 g/L lactose solution was studied in the temperature range of 65–75 °C. Lactose exhibited a stabilisation effect when similar inactivation rates as those…
View article: Editorial
Editorial Open
View article: Investigation of plant sources of hydroperoxide lyase for 2(E)-hexenal production
Investigation of plant sources of hydroperoxide lyase for 2(E)-hexenal production Open
2(E)-hexenal is a green note flavour molecule that is widely used in various compositions of aromas, flavours and perfumery. As there is considerable demand for naturally produced aromas this article deals with some aspects of this C6-vola…
View article: Application of Ionic Liquids in the Utilization of the Agricultural Wastes: Towards the One-Step Pre-Treatment and Cellulose Hydrolysis
Application of Ionic Liquids in the Utilization of the Agricultural Wastes: Towards the One-Step Pre-Treatment and Cellulose Hydrolysis Open
Cheap, renewable lignocellulosic materials are relevant to the future of biofuel production. Wood and agricultural wastes (e.g. straw, corn stover) provide a raw material source that cannot be used for human consumption, thus biofuels from…
View article: Biotransformation of acetophenone to R-1-phenylethanol with immobilized Pichia capsulata in batch reactor
Biotransformation of acetophenone to R-1-phenylethanol with immobilized Pichia capsulata in batch reactor Open
Biotransformation of acetophenone to R-1-phenylethanol with Pichia capsulata immobilized in Caalginate gel beads was studied. Experiments were carried out in a batch reactor stirred on an orbital shaker (300 rpm) at 25 ℃. Based on experime…