Milena Kordalewska
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View article: Characterization of a Novel <i>FKS1</i> Mutation in <i>Candida lusitaniae</i> Shows a Potential Critical Role for <i>MKC1</i> in Echinocandin Resistance
Characterization of a Novel <i>FKS1</i> Mutation in <i>Candida lusitaniae</i> Shows a Potential Critical Role for <i>MKC1</i> in Echinocandin Resistance Open
Caspofungin is an echinocandin antifungal that inhibits glucan synthesis in the fungal cell wall. A Candida parapsil osis bloodstream isolate resistant to echinocandins was recovered from a patient who had undergone allogeneic hematopoieti…
View article: Broad-Spectrum Activity and Mechanisms of Action of SQ109 on a Variety of Fungi
Broad-Spectrum Activity and Mechanisms of Action of SQ109 on a Variety of Fungi Open
We investigated the activity of the tuberculosis drug SQ109 against sixteen fungal pathogens: Candida albicans , C. auris , C. glabrata , C. guilliermondi , C. kefyr , C. krusei , C. lusitaniae , Candida parapsilosis , C. tropicalis, Crypt…
View article: Correction: Candida in COVID-19: Gut-Lung Axis, Dysbiosis, and Infections
Correction: Candida in COVID-19: Gut-Lung Axis, Dysbiosis, and Infections Open
View article: Candida in COVID-19: Gut-Lung Axis, Dysbiosis, and Infections
Candida in COVID-19: Gut-Lung Axis, Dysbiosis, and Infections Open
Purpose of Review This review discusses the connections between the gut-lung axis, gut and respiratory tract dysbiosis, and Candida bloodstream, oral, and respiratory infections in COVID-19 patients. Recent Findings COVID-19–related dysfun…
View article: Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance Open
We determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-re…
View article: Novel non hot spot modification in Fks1 of<i>Candida auris</i>confers echinocandin resistance
Novel non hot spot modification in Fks1 of<i>Candida auris</i>confers echinocandin resistance Open
We determined echinocandin susceptibility and FKS1 genotypes of thirteen clinical isolates of Candida auris recovered from four patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resista…
View article: Rare modification in the ergosterol biosynthesis pathway leads to amphotericin B resistance in<i>Candida auris</i>clinical isolates
Rare modification in the ergosterol biosynthesis pathway leads to amphotericin B resistance in<i>Candida auris</i>clinical isolates Open
We determined amphotericin B (AmB) susceptibility and sequenced key genes of the ergosterol biosynthesis pathway implicated in AmB resistance ( ERG2, ERG3, ERG6, ERG11 ) of 321 clinical isolates of Candida auris . In antifungal susceptibil…
View article: Emergence of Multiple SARS-CoV-2 Antibody Escape Variants in an Immunocompromised Host Undergoing Convalescent Plasma Treatment
Emergence of Multiple SARS-CoV-2 Antibody Escape Variants in an Immunocompromised Host Undergoing Convalescent Plasma Treatment Open
Over a year of the COVID-19 pandemic, distinct severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lineages have arisen in multiple geographic areas around the world. SARS-CoV-2 variants of concern (VOCs), i.e., B.1.1.7 (alpha), B…
View article: Impact of Erg11 amino acid substitutions identified in <i>Candida auris</i> clade III isolates on triazole drug susceptibility
Impact of Erg11 amino acid substitutions identified in <i>Candida auris</i> clade III isolates on triazole drug susceptibility Open
ERG11 sequencing of 28 Candida auris clade III isolates revealed the presence of concomitant V125A and F126L substitutions. Heterologous expression of Erg11-V125A/F126L in Saccharomyces cerevisiae led to reduced fluconazole and voriconazol…
View article: Antifungal Drug Susceptibility and Genetic Characterization of Fungi Recovered from COVID-19 Patients
Antifungal Drug Susceptibility and Genetic Characterization of Fungi Recovered from COVID-19 Patients Open
Fungal infections are common complications of respiratory viral infections and are associated with the increased need for intensive care and elevated mortality. Data regarding microbiological and molecular characteristics of such infection…
View article: Emergence of multiple SARS-CoV-2 antibody escape variants in an immunocompromised host undergoing convalescent plasma treatment
Emergence of multiple SARS-CoV-2 antibody escape variants in an immunocompromised host undergoing convalescent plasma treatment Open
SARS-CoV-2 Variants of Concerns (VOC), e.g., B.1.351 (20H/501Y.V2) and P1 (20J/501Y.V3), harboring N-terminal domain (NTD) or the receptor-binding domain (RBD) (e.g., E484K) mutations, exhibit reduced in vitro susceptibility to convalescen…
View article: Performance Evaluation of Culture-Independent SYBR Green Candida auris Quantitative PCR Diagnostics on Anterior Nares Surveillance Swabs
Performance Evaluation of Culture-Independent SYBR Green Candida auris Quantitative PCR Diagnostics on Anterior Nares Surveillance Swabs Open
Candida auris is a multidrug-resistant yeast that causes severe invasive infections among patients in health care facilities. C. auris was first reported in 2009 after being isolated from the ear canal of a patient in Japan and in the deca…
View article: Detection of Candida auris Antifungal Drug Resistance Markers Directly from Clinical Skin Swabs
Detection of Candida auris Antifungal Drug Resistance Markers Directly from Clinical Skin Swabs Open
Accurate and rapid assessment of Candida auris antifungal drug resistance is crucial for effective infection prevention and control actions, as well as for patient management. Here, performance of a molecular diagnostic platform, enabling …
View article: Molecular Diagnostics in the Times of Surveillance for Candida auris
Molecular Diagnostics in the Times of Surveillance for Candida auris Open
Recently, global health professionals have been significantly challenged by the emergence of Candida auris and its propensity to colonize human skin, persist in the healthcare environment, and cause healthcare-associated outbreaks. Additio…
View article: Identification of Drug Resistant Candida auris
Identification of Drug Resistant Candida auris Open
Candida auris is a multidrug resistant yeast, recognized as a cause of invasive infections and health care associated outbreaks around the world. C. auris is of great public health concern, due to its propensity for drug resi…
View article: Novel <i>FKS1</i> and <i>FKS2</i> modifications in a high-level echinocandin resistant clinical isolate of <i>Candida glabrata</i>
Novel <i>FKS1</i> and <i>FKS2</i> modifications in a high-level echinocandin resistant clinical isolate of <i>Candida glabrata</i> Open
Echinocandin resistance in Candida glabrata poses a serious clinical challenge. The underlying resistance mechanism of a pan-echinocandin-resistant C. glabrata isolate (strain L74) was investigated in this study. FKS m…
View article: Rapid Detection of <i>ERG11</i> -Associated Azole Resistance and <i>FKS</i> -Associated Echinocandin Resistance in Candida auris
Rapid Detection of <i>ERG11</i> -Associated Azole Resistance and <i>FKS</i> -Associated Echinocandin Resistance in Candida auris Open
Candida auris is an emerging multidrug-resistant yeast that can cause serious invasive infections. The accurate and rapid assessment of antifungal resistance is important for effective patient management.
View article: Spontaneous Mutational Frequency and <i>FKS</i> Mutation Rates Vary by Echinocandin Agent against <i>Candida glabrata</i>
Spontaneous Mutational Frequency and <i>FKS</i> Mutation Rates Vary by Echinocandin Agent against <i>Candida glabrata</i> Open
Echinocandins are front-line agents for treatment of invasive candidiasis. There are no reported agent-specific differences in Candida mutational frequency of resistance or propensity to develop FKS mutations.
View article: Direct Detection of Emergent Fungal Pathogen Candida auris in Clinical Skin Swabs by SYBR Green-Based Quantitative PCR Assay
Direct Detection of Emergent Fungal Pathogen Candida auris in Clinical Skin Swabs by SYBR Green-Based Quantitative PCR Assay Open
The recent emergence of the multidrug-resistant and pathogenic yeast Candida auris continues to cause public health concern worldwide. C. auris is alarming because it causes health care-associated outbreaks and can establish invasive infec…
View article: Limited <i>ERG11</i> Mutations Identified in Isolates of Candida auris Directly Contribute to Reduced Azole Susceptibility
Limited <i>ERG11</i> Mutations Identified in Isolates of Candida auris Directly Contribute to Reduced Azole Susceptibility Open
Multiple Erg11 amino acid substitutions were identified in clinical isolates of Candida auris originating from India and Colombia. Elevated azole MICs were detected in Saccharomyces cerevisiae upon heterologous expression of C. auris ERG11…
View article: Understanding Echinocandin Resistance in the Emerging Pathogen Candida auris
Understanding Echinocandin Resistance in the Emerging Pathogen Candida auris Open
Candida auris has simultaneously emerged on five continents as a fungal pathogen causing nosocomial outbreaks. The challenges in the treatment of C. auris infections are the variable antifungal susceptibility profiles among clinical isolat…
View article: <i>FKS2</i> and <i>FKS3</i> Genes of Opportunistic Human Pathogen Candida albicans Influence Echinocandin Susceptibility
<i>FKS2</i> and <i>FKS3</i> Genes of Opportunistic Human Pathogen Candida albicans Influence Echinocandin Susceptibility Open
Candida albicans , a prevailing opportunistic fungal pathogen of humans, has a diploid genome containing three homologous FKS genes that are evolutionarily conserved. One of these, the essential gene FKS1 , encodes the catalytic subunit of…
View article: A multicentre study of antifungal susceptibility patterns among 350 Candida auris isolates (2009–17) in India: role of the ERG11 and FKS1 genes in azole and echinocandin resistance
A multicentre study of antifungal susceptibility patterns among 350 Candida auris isolates (2009–17) in India: role of the ERG11 and FKS1 genes in azole and echinocandin resistance Open
Overall, 25% and 13% of isolates were MDR and multi-azole resistant, respectively. The most common resistance combination was azoles and 5-flucytosine in 14% followed by azoles and amphotericin B in 7% and azoles and echinocandins in 2% of…
View article: The Gastrointestinal Tract Is a Major Source of Echinocandin Drug Resistance in a Murine Model of Candida glabrata Colonization and Systemic Dissemination
The Gastrointestinal Tract Is a Major Source of Echinocandin Drug Resistance in a Murine Model of Candida glabrata Colonization and Systemic Dissemination Open
Candida species are a part of the human microbiome and can cause systemic infection upon immune suppression. Candida glabrata infections are increasing and have greater rates of antifungal resistance than other species. Here, we present a …
View article: Rapid and Accurate Molecular Identification of the Emerging Multidrug-Resistant Pathogen Candida auris
Rapid and Accurate Molecular Identification of the Emerging Multidrug-Resistant Pathogen Candida auris Open
Candida auris is an emerging multidrug-resistant fungal pathogen causing nosocomial and invasive infections associated with high mortality. C. auris is commonly misidentified as several different yeast species by commercially available phe…
View article: Rapid Detection of <i>FKS</i> -Associated Echinocandin Resistance in Candida glabrata
Rapid Detection of <i>FKS</i> -Associated Echinocandin Resistance in Candida glabrata Open
A novel and highly accurate diagnostic assay platform was established for rapid identification of FKS mutations associated with echinocandin resistance in Candida glabrata . The assay platform uses allele-specific molecular beacon and DNA …
View article: Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species
Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species Open
View article: PCR and real-time PCR assays to detect fungi of Alternaria alternata species
PCR and real-time PCR assays to detect fungi of Alternaria alternata species Open
Fungi of the Alternaria genus are mostly associated with allergic diseases. However, with a growing number of immunocompromised patients, these fungi, with A. alternata being the most prevalent one, are increasingly recognized as etiologic…
View article: PCR Detection of Scopulariopsis brevicaulis
PCR Detection of Scopulariopsis brevicaulis Open
Scopulariopsis brevicaulis is known as the most common etiological factor of the mould toenail infections. There are also reports indicating that S. brevicaulis could cause organ and disseminated infections. Nowadays microscopic observatio…