Miles Smith
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View article: A novel process for H&E, immunofluorescence, and imaging mass cytometry on a single slide with a concise analytics pipeline
A novel process for H&E, immunofluorescence, and imaging mass cytometry on a single slide with a concise analytics pipeline Open
Imaging mass cytometry (IMC) is a powerful spatial technology that utilizes cytometry time of flight to acquire multiplexed image datasets with up to 40 markers, via metal‐tagged antibodies. Recent advances in IMC have led to the inclusion…
View article: Ancestry-based differences in the immune phenotype are associated with lupus activity
Ancestry-based differences in the immune phenotype are associated with lupus activity Open
In this study, a broad multi-omic analysis of differences in SLE disease activity by race was used to determine differences in the cellular and molecular presentation of disease. Data from CyTOF (immunophenotyping & phospho-Cytof), multiop…
View article: OP0109 SINGLE-CELL RNA-SEQ REVEALS DIFFERENCES IN METABOLIC PATHWAYS OF MYELOID CELLS IN THE PROGRESSION OF SLE
OP0109 SINGLE-CELL RNA-SEQ REVEALS DIFFERENCES IN METABOLIC PATHWAYS OF MYELOID CELLS IN THE PROGRESSION OF SLE Open
View article: POS1155 IMPACT OF HYDROXYCHLOROQUINE ON INTERFERON PATHWAY EXPRESSION OF SLE PHENOTYPIC SUBSETS IN THE ABSENCE OF BACKGROUND MEDICATIONS
POS1155 IMPACT OF HYDROXYCHLOROQUINE ON INTERFERON PATHWAY EXPRESSION OF SLE PHENOTYPIC SUBSETS IN THE ABSENCE OF BACKGROUND MEDICATIONS Open
View article: POS1154 PRE-TREATMENT DIFFERENTIALLY EXPRESSED METAGENES CHARACTERIZE SYSTEMIC LUPUS PATIENTS WHO SUBSEQUENTLY ACHIEVE CLINICAL RESPONSE TO BELIMUMAB
POS1154 PRE-TREATMENT DIFFERENTIALLY EXPRESSED METAGENES CHARACTERIZE SYSTEMIC LUPUS PATIENTS WHO SUBSEQUENTLY ACHIEVE CLINICAL RESPONSE TO BELIMUMAB Open
View article: POS0296 GENE EXPRESSION IN SLE PATIENTS, DOMINATED BY UNIQUE B CELL PROFILES, DISTINGUISHES THOSE WITH CLINICAL IMPROVEMENT FROM ABATACEPT
POS0296 GENE EXPRESSION IN SLE PATIENTS, DOMINATED BY UNIQUE B CELL PROFILES, DISTINGUISHES THOSE WITH CLINICAL IMPROVEMENT FROM ABATACEPT Open
View article: Genetic load in incomplete lupus erythematosus
Genetic load in incomplete lupus erythematosus Open
Objective Patients with incomplete lupus erythematosus (ILE) have lupus features but insufficient criteria for SLE classification. Some patients with ILE transition to SLE, but most avoid major organ involvement. This study tested whether …
View article: Genetic load in incomplete lupus erythematosus
Genetic load in incomplete lupus erythematosus Open
Incomplete lupus erythematosus (ILE) patients have lupus features but insufficient criteria for systemic lupus erythematosus (SLE) classification. Some ILE patients transition to SLE, but most avoid major organ involvement. This study test…
View article: Ancestry-based differences in the immune system are associated with lupus severity
Ancestry-based differences in the immune system are associated with lupus severity Open
Systemic lupus erythematosus (SLE) affects 1 in 537 of African American (AA) women, which is >2-fold more than European American (EA) women. AA patients also develop the disease at a younger age, have more severe symptoms, and a greater ch…
View article: Modular gene analysis reveals distinct molecular signatures for subsets of patients with cutaneous lupus erythematosus*
Modular gene analysis reveals distinct molecular signatures for subsets of patients with cutaneous lupus erythematosus* Open
Our data support a diverse molecular profile in CLE that further adds to the clinical variations of this skin disease, and may affect disease course and treatment selection. Future studies with a larger and diverse cohort of patients with …
View article: Autoantibody-positive healthy individuals with lower lupus risk display a unique immune endotype
Autoantibody-positive healthy individuals with lower lupus risk display a unique immune endotype Open
View article: Unique Sjögren’s syndrome patient subsets defined by molecular features
Unique Sjögren’s syndrome patient subsets defined by molecular features Open
Objective To address heterogeneity complicating primary SS (pSS) clinical trials, research and care by characterizing and clustering patients by their molecular phenotypes. Methods pSS patients met American–European Consensus Group classif…
View article: 233 Autoantibody-positive healthy individuals constrain T cell pathways to regulate autoimmune disease
233 Autoantibody-positive healthy individuals constrain T cell pathways to regulate autoimmune disease Open
Background Anti-nuclear antibody (ANA) positivity is a principal feature of individuals with an autoimmune disease, yet up to one in five healthy individuals are ANA-positive (ANA+) and will never develop overt disease. Understanding diffe…
View article: 296 Activated stress response genes and perturbation of regulatory pathways in anti-nuclear antibody positive individuals and SLE patients vary by cell type and race in single-cell transcriptomic analyses
296 Activated stress response genes and perturbation of regulatory pathways in anti-nuclear antibody positive individuals and SLE patients vary by cell type and race in single-cell transcriptomic analyses Open
Background A universal hallmark of systemic lupus erythematosus (SLE) is the presence of antinuclear antibodies (ANAs). While their cognate antigens and ANA levels can associate with SLE subphenotypes, many individuals develop ANAs years p…
View article: 287 African american SLE patients with variable disease activity reveal alterations in signaling pathways and soluble mediators that are more pronounced than european american patients
287 African american SLE patients with variable disease activity reveal alterations in signaling pathways and soluble mediators that are more pronounced than european american patients Open
Background Systemic lupus erythematosus (SLE) is an autoimmune disorder with a variable clinical presentation and periods of waxing and waning disease. Heterogeneity in SLE is influenced by genetic and non-genetic susceptibility found in d…
View article: Direct Visualization of HIV-1 Replication Intermediates Shows that Capsid and CPSF6 Modulate HIV-1 Intra-nuclear Invasion and Integration
Direct Visualization of HIV-1 Replication Intermediates Shows that Capsid and CPSF6 Modulate HIV-1 Intra-nuclear Invasion and Integration Open
Direct visualization of HIV-1 replication would improve our understanding of the viral life cycle. We adapted established technology and reagents to develop an imaging approach, ViewHIV, which allows evaluation of early HIV-1 replication i…
View article: RNASEK Is a V-ATPase-Associated Factor Required for Endocytosis and the Replication of Rhinovirus, Influenza A Virus, and Dengue Virus
RNASEK Is a V-ATPase-Associated Factor Required for Endocytosis and the Replication of Rhinovirus, Influenza A Virus, and Dengue Virus Open
Human rhinovirus (HRV) causes upper respiratory infections and asthma exacerbations. We screened multiple orthologous RNAi reagents and identified host proteins that modulate HRV replication. Here, we show that RNASEK, a transmembrane prot…