Miluše Hradilová
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View article: Phenol-driven cometabolic degradation of cis-1,2-dichloroethene (cDCE): insights from Acinetobacter pittii and Ectopseudomonas alcaliphila
Phenol-driven cometabolic degradation of cis-1,2-dichloroethene (cDCE): insights from Acinetobacter pittii and Ectopseudomonas alcaliphila Open
Accumulation of xenobiotic chlorinated ethenes (CEs) at legacy industrial soil and groundwater sites around the world is a pressing environmental and public health issue. Understanding the biochemical pathways through which microorganisms …
View article: In Vitro Investigation of Statin Effects on Genes Associated with Severe COVID-19 in Cancerous and Non-Cancerous Cells
In Vitro Investigation of Statin Effects on Genes Associated with Severe COVID-19 in Cancerous and Non-Cancerous Cells Open
Background: The progressive course of coronavirus disease 2019 (COVID-19) is more frequently observed in individuals with obesity, diabetes, pulmonary and cardiovascular disease, or arterial hypertension. Many patients with these condition…
View article: Absence of fibroin H sequences and a significant divergence in the putative fibroin L homolog in Neomicropteryx cornuta (Micropterigidae) silk
Absence of fibroin H sequences and a significant divergence in the putative fibroin L homolog in Neomicropteryx cornuta (Micropterigidae) silk Open
View article: The HOX code of human adult fibroblasts reflects their ectomesenchymal or mesodermal origin
The HOX code of human adult fibroblasts reflects their ectomesenchymal or mesodermal origin Open
Fibroblasts, the most abundant cell type in the human body, play crucial roles in biological processes such as inflammation and cancer progression. They originate from the mesoderm or neural-crest-derived ectomesenchyme. Ectomesenchyme-der…
View article: Cbf11 and Mga2 function together to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast
Cbf11 and Mga2 function together to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast Open
Within a eukaryotic cell, both lipid homeostasis and faithful cell cycle progression are meticulously orchestrated. The fission yeast Schizosaccharomyces pombe provides a powerful platform to study the intricate regulatory mechanisms gover…
View article: Absence of fibroin H sequences and a significant divergence in the putative fibroin L homolog in Neomicropteryx cornuta (Lepidoptera, Micropterigidae) silk.
Absence of fibroin H sequences and a significant divergence in the putative fibroin L homolog in Neomicropteryx cornuta (Lepidoptera, Micropterigidae) silk. Open
Micropterigidae is the sister group to all other Lepidoptera, providing important insights into the evolution of Lepidoptera. However, the gene and protein profiles of silk from Micropterigidae remain unidentified. We investigated the silk…
View article: Comprehensive analysis of silk proteins and gland compartments in Limnephilus lunatus, a case-making trichopteran
Comprehensive analysis of silk proteins and gland compartments in Limnephilus lunatus, a case-making trichopteran Open
Caddisfly larvae produce silk containing heavy and light fibroins, similar to the silk of Lepidoptera, for the construction of underwater structures. We analyzed the silk of Limnephilus lunatus belonging to the case-forming suborder Integr…
View article: Cbf11 and Mga2 function as a single regulatory entity to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast
Cbf11 and Mga2 function as a single regulatory entity to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast Open
Within a eukaryotic cell, both lipid homeostasis and faithful cell cycle progression are meticulously orchestrated. The fission yeast Schizosaccharomyces pombe provides a powerful platform to study the intricate regulatory mechanisms gover…
View article: σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription
σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription Open
σ factors are considered as positive regulators of gene expression. Here we reveal the opposite, inhibitory role of these proteins. We used a combination of molecular biology methods and computational modeling to analyze the regulatory act…
View article: Unravelling heterogeneous effects of cancer‑associated fibroblasts on poor prognosis markers in breast cancer EM‑G3 cell line: <i>In vitro</i>‑targeted treatment (anti‑IL-6, anti‑VEGF-A, anti‑MFGE8) based on transcriptomic profiling
Unravelling heterogeneous effects of cancer‑associated fibroblasts on poor prognosis markers in breast cancer EM‑G3 cell line: <i>In vitro</i>‑targeted treatment (anti‑IL-6, anti‑VEGF-A, anti‑MFGE8) based on transcriptomic profiling Open
Breast cancer is the most frequently diagnosed cancer in women worldwide. Although dramatically increased survival rates of early diagnosed cases have been observed, late diagnosed patients and metastatic cancer may still be considered fat…
View article: Critical importance of DNA binding for CSL protein functions in fission yeast
Critical importance of DNA binding for CSL protein functions in fission yeast Open
CSL (CBF1/RBP-Jκ/Suppressor of Hairless/LAG-1) proteins are conserved transcription factors found in animals and fungi. In fission yeast, they regulate various cellular processes, including cell cycle progression, lipid metabolism, and cel…
View article: Characterization of silk genes in Ephestia kuehniella and Galleria mellonella revealed duplication of sericin genes and highly divergent sequences encoding fibroin heavy chains
Characterization of silk genes in Ephestia kuehniella and Galleria mellonella revealed duplication of sericin genes and highly divergent sequences encoding fibroin heavy chains Open
Silk is a secretory product of numerous arthropods with remarkable mechanical properties. In this work, we present the complete sequences of the putative major silk proteins of E. kuehniella and compare them with those of G. mellonella , w…
View article: Genome sequence and silkomics of the spindle ermine moth, Yponomeuta cagnagella, representing the early diverging lineage of the ditrysian Lepidoptera
Genome sequence and silkomics of the spindle ermine moth, Yponomeuta cagnagella, representing the early diverging lineage of the ditrysian Lepidoptera Open
View article: Mutation in Drosophila concentrative nucleoside transporter 1 alters spermatid maturation and mating behavior
Mutation in Drosophila concentrative nucleoside transporter 1 alters spermatid maturation and mating behavior Open
Concentrative nucleoside transporters (Cnts) are unidirectional carriers that mediate the energy-costly influx of nucleosides driven by the transmembrane sodium gradient. Cnts are transmembrane proteins that share a common structural organ…
View article: Using the multi-omics approach to reveal the silk composition in Plectrocnemia conspersa
Using the multi-omics approach to reveal the silk composition in Plectrocnemia conspersa Open
Similar to Lepidoptera, the larvae of Trichoptera are also capable of producing silk. Plectrocnemia conspersa , a predatory species belonging to the suborder Annulipalpia, builds massive silken retreats with preycapturing nets. In this stu…
View article: Detection of miRNAs in Patients with Abdominal Aortic Aneurysm
Detection of miRNAs in Patients with Abdominal Aortic Aneurysm Open
Introduction: MicroRNAs (miRNAs) are short non-coding ribonucleic acids that negatively regulate gene expression at the post-transcriptional level by inhibiting RNA translation or increasing mRNA degradation. miRNAs are currently considere…
View article: Exosomes produced by melanoma cells significantly influence the biological properties of normal and cancer-associated fibroblasts
Exosomes produced by melanoma cells significantly influence the biological properties of normal and cancer-associated fibroblasts Open
View article: Anaeramoebae are a divergent lineage of eukaryotes that shed light on the transition from anaerobic mitochondria to hydrogenosomes
Anaeramoebae are a divergent lineage of eukaryotes that shed light on the transition from anaerobic mitochondria to hydrogenosomes Open
View article: Predominant Biphenyl Dioxygenase From Legacy Polychlorinated Biphenyl (PCB)-Contaminated Soil Is a Part of Unusual Gene Cluster and Transforms Flavone and Flavanone
Predominant Biphenyl Dioxygenase From Legacy Polychlorinated Biphenyl (PCB)-Contaminated Soil Is a Part of Unusual Gene Cluster and Transforms Flavone and Flavanone Open
In this study, the diversity of bphA genes was assessed in a 13 C-enriched metagenome upon stable isotope probing (SIP) of microbial populations in legacy PCB-contaminated soil with 13 C-biphenyl (BP). In total, 13 bphA sequence variants (…
View article: Comparison of Silks from Pseudoips prasinana and Bombyx mori Shows Molecular Convergence in Fibroin Heavy Chains but Large Differences in Other Silk Components
Comparison of Silks from Pseudoips prasinana and Bombyx mori Shows Molecular Convergence in Fibroin Heavy Chains but Large Differences in Other Silk Components Open
Many lepidopteran larvae produce silk feeding shelters and cocoons to protect themselves and the developing pupa. As caterpillars evolved, the quality of the silk, shape of the cocoon, and techniques in forming and leaving the cocoon under…
View article: Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology
Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology Open
Euglenids represent a group of protists with diverse modes of feeding. To date, only a partial genomic sequence of Euglena gracilis and transcriptomes of several phototrophic and secondarily osmotrophic species are available, while primari…
View article: The <i>Mastigamoeba balamuthi</i> Genome and the Nature of the Free-Living Ancestor of <i>Entamoeba</i>
The <i>Mastigamoeba balamuthi</i> Genome and the Nature of the Free-Living Ancestor of <i>Entamoeba</i> Open
The transition of free-living organisms to parasitic organisms is a mysterious process that occurs in all major eukaryotic lineages. Parasites display seemingly unique features associated with their pathogenicity; however, it is important …
View article: Desmoplastic Crosstalk in Pancreatic Ductal Adenocarcinoma Is Reflected by Different Responses of Panc-1, MIAPaCa-2, PaTu-8902, and CAPAN-2 Cell Lines to Cancer-associated/Normal Fibroblasts
Desmoplastic Crosstalk in Pancreatic Ductal Adenocarcinoma Is Reflected by Different Responses of Panc-1, MIAPaCa-2, PaTu-8902, and CAPAN-2 Cell Lines to Cancer-associated/Normal Fibroblasts Open
The desmoplastic patient-specific regulation of cancer cells by CAFs (also demonstrated by the heterogeneous response of PDAC cell lines to fibroblasts) precludes simple targeting and development of an effective treatment strategy and rath…
View article: Heterotrophic Euglenid Rhabdomonas Costata Resembles Its Phototrophic Relatives in Many Aspects of Molecular and Cell Biology
Heterotrophic Euglenid Rhabdomonas Costata Resembles Its Phototrophic Relatives in Many Aspects of Molecular and Cell Biology Open
Euglenids represent a group of protists with diverse modes of feeding. To date, only a partial genomic sequence of Euglena gracilis and transcriptomes of several phototrophic and secondarily osmotrophic species are available, while primari…
View article: A rapid approach for in locus overexpression of Trypanosoma brucei proteins
A rapid approach for in locus overexpression of Trypanosoma brucei proteins Open
Altering amounts of a protein in a cell has become a crucial tool for understanding its function. In many organisms, including the protozoan parasite Trypanosoma brucei, protein overexpression has been achieved by inserting a protein-codin…
View article: Analysis of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas and Paired Normal Mucosae Reveals Cyclin D1 Deregulation and Compensatory Effect of Cyclin D2
Analysis of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas and Paired Normal Mucosae Reveals Cyclin D1 Deregulation and Compensatory Effect of Cyclin D2 Open
Aberrant regulation of the cell cycle is a typical feature of all forms of cancer. In head and neck squamous cell carcinoma (HNSCC), it is often associated with the overexpression of cyclin D1 (CCND1). However, it remains unclear how CCND1…
View article: Anti‐angiogenic effects of the blue‐green alga <i>Arthrospira platensis</i> on pancreatic cancer
Anti‐angiogenic effects of the blue‐green alga <i>Arthrospira platensis</i> on pancreatic cancer Open
Arthrospira platensis , a blue‐green alga, is a popular nutraceutical substance having potent antioxidant properties with potential anti‐carcinogenic activities. The aim of our study was to assess the possible anti‐angiogenic effects of A …
View article: The Head and Neck Squamous Cell Carcinoma Microenvironment as a Potential Target for Cancer Therapy
The Head and Neck Squamous Cell Carcinoma Microenvironment as a Potential Target for Cancer Therapy Open
Similarly to other types of malignant tumours, the incidence of head and neck cancer is increasing globally. It is frequently associated with smoking and alcohol abuse, and in a broader sense also with prolonged exposure to these factors d…
View article: Modular structure, sequence diversification and appropriate nomenclature of seroins produced in the silk glands of Lepidoptera
Modular structure, sequence diversification and appropriate nomenclature of seroins produced in the silk glands of Lepidoptera Open
View article: The expansion of genes encoding soluble silk components in the greater wax moth, Galleria mellonella
The expansion of genes encoding soluble silk components in the greater wax moth, Galleria mellonella Open
Lepidopteran silk is a complex assembly of proteins produced by a pair of highly specialized labial glands called silk glands. Silk composition has been examined only in a handful of species. Here we report on the analysis of silk gland-sp…