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View article: Applying Modern Teaching Methods and Techniques in Teaching to Meet Output Standards for Social Sciences and Humanities at the Telecommunications University – Vietnam
Applying Modern Teaching Methods and Techniques in Teaching to Meet Output Standards for Social Sciences and Humanities at the Telecommunications University – Vietnam Open
Modern teaching methods and techniques are ways of combining teaching methods and techniques with the use of information technology equipment to promote the positivity, initiative and creativity of learners, contributing to improve the qua…
View article: Single-molecule tracking reveals dual front door/back door inhibition of Cel7A cellulase by its product cellobiose
Single-molecule tracking reveals dual front door/back door inhibition of Cel7A cellulase by its product cellobiose Open
Degrading cellulose is a key step in the processing of lignocellulosic biomass into bioethanol. Cellobiose, the disaccharide product of cellulose degradation, has been shown to inhibit cellulase activity, but the mechanisms underlying prod…
View article: Single-molecule imaging of lignocellulose deconstruction by SCATTIRSTORM microscopy
Single-molecule imaging of lignocellulose deconstruction by SCATTIRSTORM microscopy Open
The goal of this project is to build a multimodal optical microscope to measure the binding, processive degradation, and pausing behaviors of cellulases as they interact with and degrade both synthetic and naturally occurring lignocellulos…
View article: Lignin impairs Cel7A degradation of in vitro lignified cellulose by impeding enzyme movement and not by acting as a sink
Lignin impairs Cel7A degradation of in vitro lignified cellulose by impeding enzyme movement and not by acting as a sink Open
Background Cellulose degradation by cellulases has been studied for decades due to the potential of using lignocellulosic biomass as a sustainable source of bioethanol. In plant cell walls, cellulose is bonded together and strengthened by …
View article: Characterization and Proteomic Analysis of <i>Geobacter sulfurreducens</i> PCA under Long-Term Electron-Donor Starvation
Characterization and Proteomic Analysis of <i>Geobacter sulfurreducens</i> PCA under Long-Term Electron-Donor Starvation Open
The natural environment of Geobacter sulfurreducens is oligotrophic and described as limiting in both electron donors and terminal electron acceptors (TEA). In previous studies we examined the effects of long-term TEA (fumarate) limitation…
View article: Lignin impairs Cel7A degradation of cellulose by impeding enzyme movement and not by acting as a sink
Lignin impairs Cel7A degradation of cellulose by impeding enzyme movement and not by acting as a sink Open
Background Cellulose degradation by cellulases has been studied for decades due to the potential of using lignocellulosic biomass as a sustainable source of bioethanol. In plant cell walls, cellulose is bonded together and strengthened by …
View article: Single-molecule tracking reveals dual front door/back door inhibition of Cel7A cellulase by its product cellobiose
Single-molecule tracking reveals dual front door/back door inhibition of Cel7A cellulase by its product cellobiose Open
Degrading cellulose is a key step in the processing of lignocellulosic biomass into bioethanol. Cellobiose, the disaccharide product of cellulose degradation, has been shown to inhibit cellulase activity, but the mechanisms underlying prod…
View article: Compositions and methods relating to transgenic plants and cellulosic ethanol production
Compositions and methods relating to transgenic plants and cellulosic ethanol production Open
Transgenic lignocellulosic plants are provided according to embodiments of the present invention, the transgenic plants transformed with an expression cassette encoding a protein operably linked to a signal peptide which targets the protei…
View article: Time-Resolved 3D Multi-Resolution Microscopy for Real-Time Cellulase Actions in Situ (Final Scientific/Technical Report)
Time-Resolved 3D Multi-Resolution Microscopy for Real-Time Cellulase Actions in Situ (Final Scientific/Technical Report) Open
In this Final Report, we are pleased to report that all technical innovation goals as described in the original grant proposal have been achieved. We also provide yet to be published results highlighting the new capabilities enabled by thi…
View article: Leveraging lifetime information to perform real-time 3D single-particle tracking in noisy environments
Leveraging lifetime information to perform real-time 3D single-particle tracking in noisy environments Open
A microscopy platform that leverages the arrival time of individual photons to enable 3D single-particle tracking of fast-moving (translational diffusion coefficient of ≃3.3 µm2/s) particles in high-background environments is reported here…
View article: Integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes
Integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes Open
We describe a multimodal microscope for visualizing processive enzymes moving on immobilized substrates. The instrument combines interference reflection microscopy (IRM) with multi-wavelength total internal reflectance fluorescence microsc…
View article: An integrated multi-wavelength SCATTIRSTORM microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes
An integrated multi-wavelength SCATTIRSTORM microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes Open
We describe a multimodal SCATTIRSTORM microscope for visualizing processive enzymes moving on immobilized substrates. The instrument combines Interference Reflection Microscopy (IRM) with multi-wavelength Total Internal Reflectance Fluores…
View article: Nanoscale Dynamics of Cellulase TrCel7A Digesting Cellulose
Nanoscale Dynamics of Cellulase TrCel7A Digesting Cellulose Open
Understanding how cellulases catalyze the digestion of lignocellulose is a major goal of bioenergy research. Cel7A from Trichoderma reesei is a model exoglucanase that degrades cellulose strands from their reducing ends by processively cle…
View article: An integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes
An integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes Open
The raw data underlying figures used in the work: An integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes
View article: Imaging Lignin Degradation: Bio-Prospecting for New Enzymes for Use In Biofuel Production
Imaging Lignin Degradation: Bio-Prospecting for New Enzymes for Use In Biofuel Production Open
The proposed research aimed at developing emission computed tomography (PET) technology to visualize the degradation of lignin, a process of central importance to biomass utilization. It is in response to a need in this area: little is kno…
View article: Convergent evolution of hetero‐oligomeric cellulose synthesis complexes in mosses and seed plants
Convergent evolution of hetero‐oligomeric cellulose synthesis complexes in mosses and seed plants Open
Summary In seed plants, cellulose is synthesized by rosette‐shaped cellulose synthesis complexes ( CSC s) that are obligate hetero‐oligomeric, comprising three non‐interchangeable cellulose synthase ( CESA ) isoforms. The moss Physcomitrel…
View article: Simulations of Cellulose Synthesis Initiation and Termination in Bacteria
Simulations of Cellulose Synthesis Initiation and Termination in Bacteria Open
The processivity of cellulose synthesis in bacterial cellulose synthase (CESA) was investigated using molecular dynamics simulations and the hybrid quantum mechanics and molecular mechanics approach. Our results suggested that cellulose sy…
View article: Domain swaps of Arabidopsis secondary wall cellulose synthases to elucidate their class specificity
Domain swaps of Arabidopsis secondary wall cellulose synthases to elucidate their class specificity Open
Cellulose microfibrils are synthesized by membrane‐embedded cellulose synthesis complexes (CSCs), currently modeled as hexamers of cellulose synthase (CESA) trimers. The three paralogous CESAs involved in secondary cell wall (SCW) cellulos…
View article: Biochemical Mechanisms and Associated Biomolecular Signatures Underlying Energy Strategies of Geobacter Sulfurreducens
Biochemical Mechanisms and Associated Biomolecular Signatures Underlying Energy Strategies of Geobacter Sulfurreducens Open
To provide the scientific understanding required to allow DOE sites to incorporate relevant biological, chemical, and physical processes into decisions concerning environmental remediation, a fundamental understanding of the controls on mi…
View article: Initiation, Elongation, and Termination of Bacterial Cellulose Synthesis
Initiation, Elongation, and Termination of Bacterial Cellulose Synthesis Open
Cellulose is the major component of the plant cell wall and composed of β-linked glucose units. Use of cellulose is greatly impacted by its physical properties, which are dominated by the number of individual cellulose strand within each f…
View article: Reducing biomass recalcitrance by heterologous expression of a bacterial peroxidase in tobacco (Nicotiana benthamiana)
Reducing biomass recalcitrance by heterologous expression of a bacterial peroxidase in tobacco (Nicotiana benthamiana) Open
Commercial scale production of biofuels from lignocellulosic feed stocks has been hampered by the resistance of plant cell walls to enzymatic conversion, primarily owing to lignin. This study investigated whether DypB, the lignin-degrading…
View article: Isolation and Characterization of Two Cellulose Morphology Mutants of Gluconacetobacter hansenii ATCC23769 Producing Cellulose with Lower Crystallinity
Isolation and Characterization of Two Cellulose Morphology Mutants of Gluconacetobacter hansenii ATCC23769 Producing Cellulose with Lower Crystallinity Open
Gluconacetobacter hansenii, a Gram-negative bacterium, produces and secrets highly crystalline cellulose into growth medium, and has long been used as a model system for studying cellulose synthesis in higher plants. Cellulose synthesis in…