Minoru Takasato
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View article: Protocol to develop a proximal tubule-on-chip model based on hiPSC-derived kidney organoids for functional analysis of renal transporters
Protocol to develop a proximal tubule-on-chip model based on hiPSC-derived kidney organoids for functional analysis of renal transporters Open
Here, we present a protocol for describing the generation of a proximal tubule-on-chip model using human induced pluripotent stem cell (hiPSC)-derived kidney organoids for renal transporter analysis. We describe steps for hiPSC differentia…
View article: Proximal tubule cell maturation rate and function are controlled by PPARα signaling in kidney organoids
Proximal tubule cell maturation rate and function are controlled by PPARα signaling in kidney organoids Open
Human pluripotent stem cell-derived kidney organoids are expected to be a useful tool for new drug discoveries, however, the immaturation of kidney organoids causes difficulties in recapitulating renal pharmacokinetics using organoids. Her…
View article: Female sex hormones inversely regulate acute kidney disease susceptibility throughout life
Female sex hormones inversely regulate acute kidney disease susceptibility throughout life Open
While epidemiological and experimental studies have demonstrated kidney-protective effects of estrogen and female sex in adulthood, some epidemiological data showed deterioration of kidney function during puberty when estrogen production i…
View article: Investigation of exon skipping therapy in kidney organoids from Alport syndrome patients derived <scp>iPSCs</scp>
Investigation of exon skipping therapy in kidney organoids from Alport syndrome patients derived <span>iPSCs</span> Open
Alport syndrome (AS) is a hereditary disease caused by mutations in the COL4A5 gene and leads to chronic kidney disease. Currently, no specific treatment has been developed. However, a recent study using AS‐model mice demonstrated that the…
View article: Identification of three distinct cell populations for urate excretion in human kidney
Identification of three distinct cell populations for urate excretion in human kidney Open
In humans, uric acid is an end-product of purine metabolism. Urate excretion from human kidney is tightly regulated by reabsorption and secretion. At least eleven genes have been identified as human renal urate transporters. However, it re…
View article: Editorial: Epithelial plasticity and complexity in development, disease and regeneration
Editorial: Epithelial plasticity and complexity in development, disease and regeneration Open
EDITORIAL article Front. Cell Dev. Biol., 12 January 2023Sec. Cell Adhesion and Migration Volume 10 - 2022 | https://doi.org/10.3389/fcell.2022.1105402
View article: Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor
Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor Open
The neural crest (NC) is an important multipotent embryonic cell population and its impaired specification leads to various developmental defects, often in an anteroposterior (A-P) axial level-specific manner. The mechanisms underlying the…
View article: Author response: Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor
Author response: Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract The neural crest (NC) …
View article: Inducing human retinal pigment epithelium-like cells from somatic tissue
Inducing human retinal pigment epithelium-like cells from somatic tissue Open
Regenerative medicine relies on basic research outcomes that are only practical when cost effective. The human eyeball requires the retinal pigment epithelium (RPE) to interface the neural retina and the choroid at large. Millions of peopl…
View article: Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor
Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor Open
The neural crest (NC) is an important multipotent embryonic cell population and its impaired specification leads to various developmental defects, often in an anteroposterior (A-P) axial level-specific manner. The mechanisms underlying the…
View article: Perspective: Extending the Utility of Three-Dimensional Organoids by Tissue Clearing Technologies
Perspective: Extending the Utility of Three-Dimensional Organoids by Tissue Clearing Technologies Open
An organoid, a self-organizing organ-like tissue developed from stem cells, can exhibit a miniaturized three-dimensional (3D) structure and part of the physiological functions of the original organ. Due to the reproducibility of tissue com…
View article: Reprogramming epiblast stem cells into pre-implantation blastocyst cell-like cells
Reprogramming epiblast stem cells into pre-implantation blastocyst cell-like cells Open
Recently, a new wave of synthetic embryo systems (SESs) has been established from cultured cells for efficient and ethical embryonic development research. We recently reported our epiblast stem cell (EPISC) reprogramming SES that generates…
View article: 27‐Hydroxycholesterol regulates human <i>SLC22A12</i> gene expression through estrogen receptor action
27‐Hydroxycholesterol regulates human <i>SLC22A12</i> gene expression through estrogen receptor action Open
The excretion and reabsorption of uric acid both to and from urine are tightly regulated by uric acid transporters. Metabolic syndrome conditions, such as obesity, hypercholesterolemia, and insulin resistance, are believed to regulate the …
View article: Genetic background, recent advances in molecular biology, and development of novel therapy in Alport syndrome
Genetic background, recent advances in molecular biology, and development of novel therapy in Alport syndrome Open
Alport syndrome (AS) is a progressive inherited kidney disease characterized by hearing loss and ocular abnormalities. There are three forms of AS depending on inheritance mode: X-linked Alport syndrome (XLAS), autosomal recessive AS (ARAS…
View article: Reprogramming Epiblast Stem Cells into Pre-Implantation Blastocyst Cell-like Cells
Reprogramming Epiblast Stem Cells into Pre-Implantation Blastocyst Cell-like Cells Open
Recently, a new wave of synthetic embryo systems (SESs) have been established from cultured cells toward efficient and ethical embryonic development research. We recently reported our epiblast stem cell (EPISC) reprogramming SES that gener…
View article: Inducing Human Retinal Pigment Epithelium-like Cells from Somatic Tissue
Inducing Human Retinal Pigment Epithelium-like Cells from Somatic Tissue Open
SUMMARY Regenerative medicine relies on basic research to find safe and useful outcomes that are only practical when cost-effective. The human eyeball requires the retinal pigment epithelium (RPE) for support and maintenance that interface…
View article: Development of an exon skipping therapy for X-linked Alport syndrome with truncating variants in COL4A5
Development of an exon skipping therapy for X-linked Alport syndrome with truncating variants in COL4A5 Open
Currently, there are no treatments for Alport syndrome, which is the second most commonly inherited kidney disease. Here we report the development of an exon-skipping therapy using an antisense-oligonucleotide (ASO) for severe male X-linke…
View article: Control and design of biosystems
Control and design of biosystems Open
Biosystems are dynamic networks driven by cross-scale interactions between molecules, cells, tissues, organs and organisms. Recent advances in our understanding of the spatiotemporal regulation and organization of biosystems have stimulate…
View article: Human URAT1/<i>SLC22A12</i>gene promoter is regulated by 27-hydroxycholesterol through estrogen response elements
Human URAT1/<i>SLC22A12</i>gene promoter is regulated by 27-hydroxycholesterol through estrogen response elements Open
Elevated levels of uric acid, a metabolite of purine in humans, is related to various diseases, such as gout, atherosclerosis and renal dysfunction. The excretion and reabsorption of uric acid to/from urine is tightly regulated by uric aci…
View article: Transcriptional evaluation of the developmental accuracy, reproducibility and robustness of kidney organoids derived from human pluripotent stem cells
Transcriptional evaluation of the developmental accuracy, reproducibility and robustness of kidney organoids derived from human pluripotent stem cells Open
We have previously reported a protocol for the directed differentiation of human induced pluripotent stem cells to kidney organoids comprised of nephrons, proximal and distal epithelium, vasculature and surrounding interstitial elements. T…