Miriam Belmonte
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View article: Selection of pre-leukemic hematopoietic stem cells driven by distinct extracellular matrix molecules
Selection of pre-leukemic hematopoietic stem cells driven by distinct extracellular matrix molecules Open
Summary Despite rapid advances in mapping genetic drivers and gene expression changes in hematopoietic stem cells (HSCs), there is a relative paucity of studies at the protein level. Here, we perform a deep, multi-omic characterization (ep…
View article: Increased CXCL10 (IP-10) is associated with advanced myeloproliferative neoplasms and its loss dampens erythrocytosis in mouse models
Increased CXCL10 (IP-10) is associated with advanced myeloproliferative neoplasms and its loss dampens erythrocytosis in mouse models Open
Key studies in pre-leukemic disorders have linked increases in pro-inflammatory cytokines with accelerated phases of the disease, but the precise role of the cellular microenvironment in disease initiation and evolution remains poorly unde…
View article: Convergent somatic evolution commences in utero in a germline ribosomopathy
Convergent somatic evolution commences in utero in a germline ribosomopathy Open
View article: Identification and characterization of <i>in vitro</i> expanded hematopoietic stem cells
Identification and characterization of <i>in vitro</i> expanded hematopoietic stem cells Open
View article: Diverse mutational landscapes in human lymphocytes
Diverse mutational landscapes in human lymphocytes Open
View article: Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division
Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division Open
This protocol details the isolation and in vitro maintenance of single hematopoietic stem cells (HSCs) in the absence of the bone marrow niche. The HSCs do not divide over 7 days and fully retain their long-term functional capacity …
View article: A highly efficient reporter system for identifying and characterizing <i>in vitro</i> expanded hematopoietic stem cells
A highly efficient reporter system for identifying and characterizing <i>in vitro</i> expanded hematopoietic stem cells Open
Summary Hematopoietic stem cells (HSCs) cultured outside the body are the fundamental component of a wide range of cellular and gene therapies. Recent efforts have achieved more than 200-fold expansion of functional HSCs, but their molecul…
View article: Hematopoietic stem cells retain functional potential and molecular identity in hibernation cultures
Hematopoietic stem cells retain functional potential and molecular identity in hibernation cultures Open
Advances in the isolation and gene expression profiling of single hematopoietic stem cells (HSCs) have permitted in-depth resolution of their molecular program. However, long-term HSCs can only be isolated to near purity from adult mouse b…
View article: Genome-wide mutational signatures of immunological diversification in normal lymphocytes
Genome-wide mutational signatures of immunological diversification in normal lymphocytes Open
A lymphocyte suffers many threats to its genome, including programmed mutation during differentiation, antigen-driven proliferation and residency in diverse microenvironments. After developing protocols for single-cell lymphocyte expansion…
View article: Response to Collinson et al. Comment
Response to Collinson et al. Comment Open
In response to our recent paper which identified GRO-α, EGF and Eotaxin as potential biomarkers in chronic phase myeloproliferative neoplasms,1 Collinson et al explored one of the possible cellular sources of these molecules by measuring l…
View article: Longitudinal Cytokine Profiling Identifies GRO‐α and EGF as Potential Biomarkers of Disease Progression in Essential Thrombocythemia
Longitudinal Cytokine Profiling Identifies GRO‐α and EGF as Potential Biomarkers of Disease Progression in Essential Thrombocythemia Open
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View article: Population dynamics of normal human blood inferred from somatic mutations
Population dynamics of normal human blood inferred from somatic mutations Open
View article: Single-cell approaches identify the molecular network driving malignant hematopoietic stem cell self-renewal
Single-cell approaches identify the molecular network driving malignant hematopoietic stem cell self-renewal Open
Key Points Single-cell approaches identify regulators of malignant HSC self-renewal. Identification of novel roles for Bmi1, Pbx1, and Meis1 in myeloproliferative neoplasms.
View article: Targeting Leukemia Stem Cells: Which Pathways Drive Self-Renewal Activity in T-Cell Acute Lymphoblastic Leukemia?
Targeting Leukemia Stem Cells: Which Pathways Drive Self-Renewal Activity in T-Cell Acute Lymphoblastic Leukemia? Open
T-Cell acute lymphoblastic leukemia (T-ALL) is a malignancy of white blood cells, characterized by an uncontrolled accumulation of T-cell progenitors. During leukemic progression, immature T cells grow abnormally and crowd into the bone ma…
View article: Leukemia stem cells in T-ALL require active Hif1α and Wnt signaling
Leukemia stem cells in T-ALL require active Hif1α and Wnt signaling Open
Key Points A real-time, integrated fluorescent Wnt reporter marks rare leukemia stem cells in T-ALL. Deletion of β-catenin or Hif1α reduces LIC frequency in established tumors, but does not affect the growth of bulk cells.