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View article: Dynamics of Tpm1.8 domains on actin filaments with single-molecule resolution
Dynamics of Tpm1.8 domains on actin filaments with single-molecule resolution Open
Characterization of the kinetics of Tpm1.8 binding to actin filaments with single-molecule resolution. This work provides molecular insight into actin–tropomyosin filament formation and the role of tropomyosins in regulating actin filament…
View article: Dynamics of Tpm1.8 domains on actin filaments with single molecule resolution
Dynamics of Tpm1.8 domains on actin filaments with single molecule resolution Open
Tropomyosins regulate dynamics and functions of the actin cytoskeleton by forming long chains along the two strands of actin filaments that act as gatekeepers for the binding of other actin-binding proteins. The fundamental molecular inter…
Interactions of tropomyosin Tpm1.1 on a single actin filament: A method for extraction and processing of high resolution TIRF microscopy data Open
Skeletal muscle tropomyosin (Tpm1.1) is an elongated, rod-shaped, alpha-helical coiled-coil protein that forms continuous head-to-tail polymers along both sides of the actin filament. In this study we use single molecule fluorescence TIRF …
Ultralow- and Low-Background Surfaces for Single-Molecule Localization Microscopy of Multistep Biointerfaces for Single-Molecule Sensing Open
Single-molecule localization microscopy (SMLM) has created the opportunity of pushing fluorescence microscopy from being a biological imaging tool to a surface characterization and possibly even a quantitative analytical tool. The latter c…
Effect of surface chemistry on tropomyosin binding to actin filaments on surfaces Open
Reconstitution of actin filaments on surfaces for observation of filament‐associated protein dynamics by fluorescence microscopy is currently an exciting field in biophysics. Here we examine the effects of attaching actin filaments to surf…
The impact of tropomyosins on actin filament assembly is isoform specific Open
Tropomyosin (Tpm) is an α helical coiled-coil dimer that forms a co-polymer along the actin filament. Tpm is involved in the regulation of actin's interaction with binding proteins as well as stabilization of the actin filament and its ass…
A small molecule inhibitor of tropomyosin dissociates actin binding from tropomyosin-directed regulation of actin dynamics Open
The tropomyosin family of proteins form end-to-end polymers along the actin filament. Tumour cells rely on specific tropomyosin-containing actin filament populations for growth and survival. To dissect out the role of tropomyosin in actin …