Molly S. Shoichet
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View article: Immunomodulation With Local, Sustained Delivery of Pituitary Adenylate Cyclase Activating Polypeptide Results in Improved Functional Recovery in Stroke‐Injured Mice
Immunomodulation With Local, Sustained Delivery of Pituitary Adenylate Cyclase Activating Polypeptide Results in Improved Functional Recovery in Stroke‐Injured Mice Open
Following ischemic stroke, astrocytes and microglia become activated and create a hostile microenvironment that can exacerbate brain damage, yet these cells also contribute to tissue regeneration. Pituitary adenylate cyclase activating pol…
View article: Identification of Injury-Response State Determinants in Glioblastoma Stem Cells
Identification of Injury-Response State Determinants in Glioblastoma Stem Cells Open
Glioblastoma (GBM) is a common and highly lethal type of primary brain tumor in adults. Therapeutic failure is partly attributed to a fraction of Glioblastoma Stem Cells (GSCs) that show high levels of heterogeneity and plasticity. GSCs ex…
View article: Colloid‐Forming Prodrug‐Hydrogel Composite Prolongs Lower Intraocular Pressure in Rodent Eyes after Subconjunctival Injection
Colloid‐Forming Prodrug‐Hydrogel Composite Prolongs Lower Intraocular Pressure in Rodent Eyes after Subconjunctival Injection Open
Colloidal drug aggregates (CDAs) are challenging in drug discovery due to their unpredictable formation and interference with screening assays. These limitations are turned into a strategic advantage by leveraging CDAs as a drug delivery p…
View article: RNA lipid nanoparticles stabilized during nebulization through excipient selection
RNA lipid nanoparticles stabilized during nebulization through excipient selection Open
High shear forces during nebulization can destabilize lipid nanoparticles with encapsulated RNA and lead to material loss. We demonstrated the impact of nebulization buffer composition on nanoparticle size, RNA encapsulation, and material …
View article: Acid‐Responsive Polymer Additives Increase RNA Transfection from Lipid Nanoparticles
Acid‐Responsive Polymer Additives Increase RNA Transfection from Lipid Nanoparticles Open
Lipid nanoparticles (LNPs) are the most clinically advanced RNA delivery technology, but their efficiency is limited by low RNA release after endosome disruption. To improve RNA release, an acid‐responsive polymer is synthesized with which…
View article: Hyaluronan improves photoreceptor differentiation and maturation in human retinal organoids
Hyaluronan improves photoreceptor differentiation and maturation in human retinal organoids Open
Human stem cell-derived organoids enable both disease modeling and serve as a source of cells for transplantation. Human retinal organoids are particularly important as a source of human photoreceptors; however, the long differentiation pe…
View article: Transplanted human photoreceptors transfer cytoplasmic material but not to the recipient mouse retina
Transplanted human photoreceptors transfer cytoplasmic material but not to the recipient mouse retina Open
Background The discovery of material transfer between transplanted and host mouse photoreceptors has expanded the possibilities for utilizing transplanted photoreceptors as potential vehicles for delivering therapeutic cargo. However, prev…
View article: Engineering Biomaterials to Model Immune‐Tumor Interactions In Vitro
Engineering Biomaterials to Model Immune‐Tumor Interactions In Vitro Open
Engineered biomaterial scaffolds are becoming more prominent in research laboratories to study drug efficacy for oncological applications in vitro, but do they have a place in pharmaceutical drug screening pipelines? The low efficacy of ca…
View article: Long‐Acting Ocular Injectables: Are We Looking In The Right Direction?
Long‐Acting Ocular Injectables: Are We Looking In The Right Direction? Open
The complex anatomy and physiological barriers of the eye make delivering ocular therapeutics challenging. Generally, effective drug delivery to the eye is hindered by rapid clearance and limited drug bioavailability. Biomaterial‐based app…
View article: Soluble CX3CL1-expressing retinal pigment epithelium cells protect rod photoreceptors in a mouse model of retinitis pigmentosa
Soluble CX3CL1-expressing retinal pigment epithelium cells protect rod photoreceptors in a mouse model of retinitis pigmentosa Open
View article: Engineered In Vitro Tumor Model Recapitulates Molecular Signatures of Invasion in Glioblastoma
Engineered In Vitro Tumor Model Recapitulates Molecular Signatures of Invasion in Glioblastoma Open
Glioblastoma stem cells (GSCs) play an important role in the invasive nature of glioblastoma (GBM); yet, the mechanisms driving this behavior are poorly understood. To recapitulate tumor invasion in vitro, we developed a GBM tumor-mimetic …
View article: Tissue‐Engineered Disease Modeling of Lymphangioleiomyomatosis Exposes a Therapeutic Vulnerability to HDAC Inhibition
Tissue‐Engineered Disease Modeling of Lymphangioleiomyomatosis Exposes a Therapeutic Vulnerability to HDAC Inhibition Open
Lymphangioleiomyomatosis (LAM) is a rare disease involving cystic lung destruction by invasive LAM cells. These cells harbor loss‐of‐function mutations in TSC2 , conferring hyperactive mTORC1 signaling. Here, tissue engineering tools are e…
View article: Data from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis
Data from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis Open
Lymphangioleiomyomatosis (LAM) is a progressive destructive neoplasm of the lung associated with inactivating mutations in the TSC1 or TSC2 tumor suppressor genes. Cell or animal models that accurately reflect the pathology o…
View article: Supplementary Figures S1-S7 from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis
Supplementary Figures S1-S7 from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis Open
This file provides supplementary data pertaining to TSC2 mutation and protein expression analysis in human fibroblast cell lines (Figure S1); as well as Karyotypes (Figure S2), in vitro and in vivo pluripotency and differentiation potentia…
View article: Supplementary Figures S1-S7 from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis
Supplementary Figures S1-S7 from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis Open
This file provides supplementary data pertaining to TSC2 mutation and protein expression analysis in human fibroblast cell lines (Figure S1); as well as Karyotypes (Figure S2), in vitro and in vivo pluripotency and differentiation potentia…
View article: Supplementary Materials and Methods and References from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis
Supplementary Materials and Methods and References from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis Open
This file contains descriptions of materials and methods used in this study pertaining to cell and tissue staining, Western blot and mRNA analysis, and cell size and genotyping analyses. Corresponding references are also included.
View article: Data from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis
Data from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis Open
Lymphangioleiomyomatosis (LAM) is a progressive destructive neoplasm of the lung associated with inactivating mutations in the TSC1 or TSC2 tumor suppressor genes. Cell or animal models that accurately reflect the pathology o…
View article: Supplementary Figure Legends from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis
Supplementary Figure Legends from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis Open
This file contains the full descriptive legends for Supplementary Figures S1-S7.
View article: Supplementary Figure Legends from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis
Supplementary Figure Legends from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis Open
This file contains the full descriptive legends for Supplementary Figures S1-S7.
View article: Supplementary Materials and Methods and References from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis
Supplementary Materials and Methods and References from Human Pluripotent Stem Cell–Derived <i>TSC2</i>-Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis Open
This file contains descriptions of materials and methods used in this study pertaining to cell and tissue staining, Western blot and mRNA analysis, and cell size and genotyping analyses. Corresponding references are also included.
View article: Synthetic Ionizable Colloidal Drug Aggregates Enable Endosomal Disruption
Synthetic Ionizable Colloidal Drug Aggregates Enable Endosomal Disruption Open
Colloidal drug aggregates enable the design of drug‐rich nanoparticles; however, the efficacy of stabilized colloidal drug aggregates is limited by entrapment in the endo‐lysosomal pathway. Although ionizable drugs are used to elicit lysos…
View article: Viscoelastic Notch Signaling Hydrogel Induces Liver Bile Duct Organoid Growth and Morphogenesis
Viscoelastic Notch Signaling Hydrogel Induces Liver Bile Duct Organoid Growth and Morphogenesis Open
Cholangiocyte organoids can be used to model liver biliary disease; however, both a defined matrix in which to emulate cholangiocyte self-assembly and the mechano-transduction pathways involved therein remain elusive. We designed a series …
View article: Synthesis and adsorption of polymers :: control of polymer and surface structure/
Synthesis and adsorption of polymers :: control of polymer and surface structure/ Open
Polymer surface modification can be accomplished by several techniques including chemical reaction and adsorption. In Chapter I, a simple and versatile technique to introduce carboxylic acid functionality to the surfaces of three fluoropol…
View article: Three-dimensional drug screen identifies HDAC inhibitors as therapeutic agents in mTORC1<i>-</i>driven lymphangioleiomyomatosis
Three-dimensional drug screen identifies HDAC inhibitors as therapeutic agents in mTORC1<i>-</i>driven lymphangioleiomyomatosis Open
Lymphangioleiomyomatosis (LAM) is a rare disease involving cystic lung destruction by invasive LAM cells. These cells harbor loss-of-function mutations in TSC2 , conferring constitutive mTORC1 signaling. Rapamycin is the only clinically ap…
View article: Safe and immune-tolerant 'designer' RPE cells towards the treatment of age-related macular degeneration
Safe and immune-tolerant 'designer' RPE cells towards the treatment of age-related macular degeneration Open
View article: Single-cell chromatin accessibility profiling of glioblastoma identifies an invasive cancer stem cell population associated with lower survival
Single-cell chromatin accessibility profiling of glioblastoma identifies an invasive cancer stem cell population associated with lower survival Open
Chromatin accessibility discriminates stem from mature cell populations, enabling the identification of primitive stem-like cells in primary tumors, such as glioblastoma (GBM) where self-renewing cells driving cancer progression and recurr…
View article: Author response: Single-cell chromatin accessibility profiling of glioblastoma identifies an invasive cancer stem cell population associated with lower survival
Author response: Single-cell chromatin accessibility profiling of glioblastoma identifies an invasive cancer stem cell population associated with lower survival Open
View article: An Injectable Hyaluronan–Methylcellulose (HAMC) Hydrogel Combined with Wharton’s Jelly-Derived Mesenchymal Stromal Cells (WJ-MSCs) Promotes Degenerative Disc Repair
An Injectable Hyaluronan–Methylcellulose (HAMC) Hydrogel Combined with Wharton’s Jelly-Derived Mesenchymal Stromal Cells (WJ-MSCs) Promotes Degenerative Disc Repair Open
Intervertebral disc (IVD) degeneration is one of the predominant causes of chronic low back pain (LBP), which is a leading cause of disability worldwide. Despite substantial progress in cell therapy for the treatment of IVD degeneration, s…
View article: Reengineering biocatalysts: Computational redesign of chondroitinase ABC improves efficacy and stability
Reengineering biocatalysts: Computational redesign of chondroitinase ABC improves efficacy and stability Open
Computational redesign of chondroitinase ABC introduces 37 point mutations to improve enzyme stability and activity.
View article: In vitro Evaluation of ASCs and HUVECs Co-cultures in 3D Biodegradable Hydrogels on Neurite Outgrowth and Vascular Organization
In vitro Evaluation of ASCs and HUVECs Co-cultures in 3D Biodegradable Hydrogels on Neurite Outgrowth and Vascular Organization Open
Vascular disruption following spinal cord injury (SCI) decisively contributes to the poor functional recovery prognosis facing patients with the condition. Using a previously developed gellan gum hydrogel to which the adhesion motif GRGDS …