Monika W. Murcha
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View article: Structural and Functional Perspectives on Mitochondrial <scp>LYR</scp>‐Domain Proteins in Plants
Structural and Functional Perspectives on Mitochondrial <span>LYR</span>‐Domain Proteins in Plants Open
Arabidopsis thaliana contains 12 nuclear‐encoded mitochondrial LYR (leucine/tyrosine/arginine) motif‐containing proteins (LYRMs). Four of these proteins have been previously characterized in plants and were found to be involved in iron–sul…
View article: The complex I subunit <scp>B22</scp> contains a <scp>LYR</scp> domain that is crucial for an interaction with the mitochondrial acyl carrier protein <scp>SDAP1</scp>
The complex I subunit <span>B22</span> contains a <span>LYR</span> domain that is crucial for an interaction with the mitochondrial acyl carrier protein <span>SDAP1</span> Open
SUMMARY Mitochondrial complex I (CI), a large multi‐subunit respiratory complex contains two LYR (leucine/tyrosine/arginine) domain‐containing subunits, B14 (NDUA6/LYRM6) and B22 (NDUB9/LYRM3). Mitochondrial LYR (LYRM) proteins are soluble…
View article: The lowdown on breakdown: Open questions in plant proteolysis
The lowdown on breakdown: Open questions in plant proteolysis Open
Proteolysis, including post-translational proteolytic processing as well as protein degradation and amino acid recycling, is an essential component of the growth and development of living organisms. In this article, experts in plant proteo…
View article: Structural analysis of the SAM domain of the Arabidopsis mitochondrial tRNA import receptor
Structural analysis of the SAM domain of the Arabidopsis mitochondrial tRNA import receptor Open
View article: Structural analysis of the Sterile alpha motif (SAM) domain of the Arabidopsis mitochondrial tRNA import receptor
Structural analysis of the Sterile alpha motif (SAM) domain of the Arabidopsis mitochondrial tRNA import receptor Open
Mitochondria are membrane bound organelles of endosymbiotic origin with limited protein coding capacity. As a consequence, the continual import of nuclear-encoded protein and nucleic acids such as DNA and small non-coding RNA is required a…
View article: FTSH PROTEASE 3 facilitates Complex I degradation through a direct interaction with the Complex I subunit PSST
FTSH PROTEASE 3 facilitates Complex I degradation through a direct interaction with the Complex I subunit PSST Open
Complex I (CI) (NADH dehydrogenase), the largest complex involved in mitochondrial oxidative phosphorylation, is composed of nuclear- and mitochondrial-encoded subunits. CI assembly occurs via the sequential addition of subdomains and modu…
View article: FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSST
FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSST Open
Complex I (NADH dehydrogenase), the largest complex involved in mitochondrial oxidative phosphorylation is composed of nuclear and mitochondrial encoded subunits. Its assembly requires sequential addition of subdomains and modules. As it i…
View article: The biogenesis and regulation of the plant oxidative phosphorylation system
The biogenesis and regulation of the plant oxidative phosphorylation system Open
Mitochondria are central organelles for respiration in plants. At the heart of this process is oxidative phosphorylation (OXPHOS) system, which generates ATP required for cellular energetic needs. OXPHOS complexes comprise of multiple subu…
View article: Presequence translocase-associated motor subunits of the mitochondrial protein import apparatus are dual-targeted to mitochondria and plastids
Presequence translocase-associated motor subunits of the mitochondrial protein import apparatus are dual-targeted to mitochondria and plastids Open
The import and assembly of most of the mitochondrial proteome is regulated by protein translocases located within the mitochondrial membranes. The Presequence Translocase-Associated Motor (PAM) complex powers the translocation of proteins …
View article: An MCIA-like complex is required for mitochondrial complex I assembly and seed development in maize
An MCIA-like complex is required for mitochondrial complex I assembly and seed development in maize Open
View article: Mitochondrial activity and biogenesis during resurrection of <i>Haberlea rhodopensis</i>
Mitochondrial activity and biogenesis during resurrection of <i>Haberlea rhodopensis</i> Open
Summary Haberlea rhodopensis is a resurrection plant that can tolerate extreme and prolonged periods of desiccation with a rapid restoration of physiological function upon rehydration. Specialized mechanisms are required to minimize cellul…
View article: Late Embryogenesis Abundant (LEA)5 Regulates Translation in Mitochondria and Chloroplasts to Enhance Growth and Stress Tolerance
Late Embryogenesis Abundant (LEA)5 Regulates Translation in Mitochondria and Chloroplasts to Enhance Growth and Stress Tolerance Open
The late embryogenesis abundant (LEA)5 protein is predominantly expressed in Arabidopsis leaves in the dark, the levels of LEA5 transcripts decreasing rapidly upon illumination. LEA5 is important in plant responses to environmental stresse…
View article: Proteolytic regulation of mitochondrial oxidative phosphorylation components in plants
Proteolytic regulation of mitochondrial oxidative phosphorylation components in plants Open
Mitochondrial function relies on the homeostasis and quality control of their proteome, including components of the oxidative phosphorylation (OXPHOS) pathway that generates energy in form of ATP. OXPHOS subunits are under constant exposur…
View article: The mitochondrial <scp>LYR</scp> protein <scp>SDHAF1</scp> is required for succinate dehydrogenase activity in Arabidopsis
The mitochondrial <span>LYR</span> protein <span>SDHAF1</span> is required for succinate dehydrogenase activity in Arabidopsis Open
Summary Succinate dehydrogenase (SDH, complex II), which plays an essential role in mitochondrial respiration and tricarboxylic acid metabolism, requires the assembly of eight nuclear‐encoded subunits and the insertion of various cofactors…
View article: Inhibition of chloroplast translation as a new target for herbicides
Inhibition of chloroplast translation as a new target for herbicides Open
The antibiotic linezolid is an effective herbicide pre- and post-emergence. Making a series of analogues, we partly separated its antibacterial and herbicidal activities, making chloroplast translation a potential new herbicide mode of act…
View article: Inhibition of chloroplast translation as a new target for herbicides
Inhibition of chloroplast translation as a new target for herbicides Open
The rise in herbicide resistance over recent decades threatens global agriculture and food security and so discovery of new modes of action is increasingly important. Here we reveal linezolid, an oxazolidinone antibiotic that inhibits micr…
View article: The mitochondrial AAA protease FTSH3 regulates Complex I abundance by promoting its disassembly
The mitochondrial AAA protease FTSH3 regulates Complex I abundance by promoting its disassembly Open
ATP is generated in mitochondria by oxidative phosphorylation. Complex I (NADH:ubiquinone oxidoreductase or NADH dehydrogenase) is the first multisubunit protein complex of this pathway, oxidizing NADH and transferring electrons to the ubi…
View article: Current status of the multinational Arabidopsis community
Current status of the multinational Arabidopsis community Open
The multinational Arabidopsis research community is highly collaborative and over the past thirty years these activities have been documented by the Multinational Arabidopsis Steering Committee (MASC). Here, we (a) highlight recent researc…
View article: Mitochondrial CLPP2 Assists Coordination and Homeostasis of Respiratory Complexes
Mitochondrial CLPP2 Assists Coordination and Homeostasis of Respiratory Complexes Open
Protein homeostasis in eukaryotic organelles and their progenitor prokaryotes is regulated by a series of proteases including the caseinolytic protease (CLPP). CLPP has essential roles in chloroplast biogenesis and maintenance, but the sig…
View article: Coordination and assembly of protein complexes encoded across mitochondrial and nuclear genomes is assisted by CLPP2 in<i>Arabidopsis thaliana</i>
Coordination and assembly of protein complexes encoded across mitochondrial and nuclear genomes is assisted by CLPP2 in<i>Arabidopsis thaliana</i> Open
Protein homeostasis in eukaryotic organelles and their progenitor prokaryotes is regulated by a series of proteases including the caseinolytic protease (CLPP). CLPP has essential roles in chloroplast biogenesis and maintenance, but the sig…
View article: A Mitochondrial LYR Protein Is Required for Complex I Assembly
A Mitochondrial LYR Protein Is Required for Complex I Assembly Open
Complex I biogenesis requires the expression of both nuclear and mitochondrial genes, the import of proteins, cofactor biosynthesis, and the assembly of at least 49 individual subunits. Assembly factors interact with subunits of Complex I …
View article: The peptidases involved in plant mitochondrial protein import
The peptidases involved in plant mitochondrial protein import Open
Mitochondrial biogenesis requires correct targeting and import of nuclear-encoded proteins to ensure the mitochondrial proteome responds to meet the plant’s energetic demands. Protein-degrading machineries also play key roles in protein im…
View article: Accumulation of endogenous peptides triggers a pathogen stress response in <i>Arabidopsis thaliana</i>
Accumulation of endogenous peptides triggers a pathogen stress response in <i>Arabidopsis thaliana</i> Open
Summary The stepwise degradation of peptides to amino acids in plant mitochondria and chloroplasts is catalyzed by a network of oligopeptidases (presequence protease PreP, organellar oligopeptidase OOP ) and aminopeptidases. In the present…
View article: An Assembly Factor Promotes Assembly of Flavinated SDH1 into the Succinate Dehydrogenase Complex
An Assembly Factor Promotes Assembly of Flavinated SDH1 into the Succinate Dehydrogenase Complex Open
Succinate dehydrogenase (Complex II; SDH) plays an important role in mitochondrial respiratory metabolism. The SDH complex consists of four core subunits and multiple cofactors, which must be assembled correctly to ensure enzyme function. …
View article: Targeting plant <scp>DIHYDROFOLATE REDUCTASE</scp> with antifolates and mechanisms for genetic resistance
Targeting plant <span>DIHYDROFOLATE REDUCTASE</span> with antifolates and mechanisms for genetic resistance Open
Summary The folate biosynthetic pathway and its key enzyme dihydrofolate reductase ( DHFR ) is a popular target for drug development due to its essential role in the synthesis of DNA precursors and some amino acids. Despite its importance,…
View article: Isolation and Respiratory Measurements of Mitochondria from <em>Arabidopsis thaliana</em>
Isolation and Respiratory Measurements of Mitochondria from <em>Arabidopsis thaliana</em> Open
Mitochondria are essential organelles involved in numerous metabolic pathways in plants, most notably the production of adenosine triphosphate (ATP) from the oxidation of reduced compounds such as nicotinamide adenine dinucleotide (NADH) a…
View article: A Common Peptidolytic Mechanism for Targeting Peptide Degradation in Mitochondria and Chloroplasts
A Common Peptidolytic Mechanism for Targeting Peptide Degradation in Mitochondria and Chloroplasts Open
View article: The plant <i>i</i>-AAA protease controls the turnover of an essential mitochondrial protein import component
The plant <i>i</i>-AAA protease controls the turnover of an essential mitochondrial protein import component Open
Mitochondria are multifunctional organelles that play a central role in energy metabolism. Owing to the life-essential functions of these organelles, mitochondrial content, quality and dynamics are tightly controlled. Across the species, h…
View article: Plant-Specific Preprotein and Amino Acid Transporter Proteins Are Required for tRNA Import into Mitochondria
Plant-Specific Preprotein and Amino Acid Transporter Proteins Are Required for tRNA Import into Mitochondria Open
A variety of eukaryotes, in particular plants, do not contain the required number of tRNAs to support the translation of mitochondria-encoded genes and thus need to import tRNAs from the cytosol. This study identified two Arabidopsis (Arab…
View article: Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of<i>Arabidopsis thaliana</i>
Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of<i>Arabidopsis thaliana</i> Open
In plant cells, mitochondria are major providers of energy and building blocks for growth and development as well as abiotic and biotic stress responses. They are encircled by two lipid membranes containing proteins that control mitochondr…