Moshe Szyf
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View article: The epiMelanoma test enables plasma-based detection of melanoma and prediction of immunotherapy response
The epiMelanoma test enables plasma-based detection of melanoma and prediction of immunotherapy response Open
The epiMelanoma assay represents a promising non-invasive tool for early melanoma detection and personalized treatment, by enabling timely intervention and identifying patients most likely to benefit from immunotherapy, and therefore, pote…
View article: DNA Methylation, Prenatal Maternal Stress and the Broad Autism Phenotype in Adolescence: Project Ice Storm
DNA Methylation, Prenatal Maternal Stress and the Broad Autism Phenotype in Adolescence: Project Ice Storm Open
Background/Objectives. Prenatal maternal stress (PNMS) predicts risk for autism spectrum disorders (ASD) although the mechanisms are unknown. The aim of this study was to determine the extent to which DNA methylation mediates the effects o…
View article: EpiAge: a next-generation sequencing-based ELOVL2 epigenetic clock for biological age assessment in saliva and blood across health and disease
EpiAge: a next-generation sequencing-based ELOVL2 epigenetic clock for biological age assessment in saliva and blood across health and disease Open
This study introduces EpiAgePublic, a new method to estimate biological age using only three specific sites on the gene ELOVL2, known for its connection to aging. Unlike traditional methods that require complex and extensive data, our mode…
View article: Hippocampal reelin and GAD67 gene expression and methylation in the GFAP.HMOX1 mouse model of schizophrenia
Hippocampal reelin and GAD67 gene expression and methylation in the GFAP.HMOX1 mouse model of schizophrenia Open
Schizophrenia is a complex neuropsychiatric disorder featuring enhanced brain oxidative stress and deficient reelin protein. GFAP.HMOX10-12m mice that overexpress heme oxygenase-1 (HO-1) in astrocytes manifest a schizophrenia-like neuroche…
View article: Clinical validation of peripheral blood mononuclear cell DNA methylation markers for accurate early detection of hepatocellular carcinoma in Asian patients
Clinical validation of peripheral blood mononuclear cell DNA methylation markers for accurate early detection of hepatocellular carcinoma in Asian patients Open
Our study confirms that combining gene methylation profiles with established diagnostic markers substantially improves the sensitivity of detecting early-stage HCC. This integrated diagnostic approach holds promise for advancing non-invasi…
View article: Correction: Potential reversal of epigenetic age using a diet and lifestyle intervention: a pilot randomized clinical trial
Correction: Potential reversal of epigenetic age using a diet and lifestyle intervention: a pilot randomized clinical trial Open
Aging | doi:10.18632/aging.205700. Kara N. Fitzgerald, Romilly Hodges, Douglas Hanes, Emily Stack, David Cheishvili, Moshe Szyf, Janine Henkel, Melissa W. Twedt, Despina Giannopoulou, Josette Herdell, Sally Logan, Ryan Bradley
View article: Genetic confounds of transgenerational epigenetic inheritance in mice
Genetic confounds of transgenerational epigenetic inheritance in mice Open
Transgenerational epigenetic inheritance in mammals remains a controversial phenomenon. A recent study by Takahashi et al. provides evidence for this mode of inheritance in mice by using a CRISPR/Cas9-based epigenetic editing technique to …
View article: Validation of novel <scp>DNA</scp> methylation markers in cervical precancer and cancer
Validation of novel <span>DNA</span> methylation markers in cervical precancer and cancer Open
We have recently identified, using a genome‐wide approach, new methylation markers which were evaluated among various cervical intraepithelial neoplasia (CIN) grades and cervical cancer. We herein validate the methylated state of these gen…
View article: Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance
Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance Open
As advances in genome engineering inch the technology towards wider clinical use—slowed by technical and ethical hurdles—a newer offshoot, termed “epigenome engineering”, offers the ability to correct disease-causing changes in the DNA wit…
View article: Supplementary Table 2 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Table 2 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 71KB, Supplementary Table S2. A list of kinases whose phosphorylation was examined in SkHep1 liver cancer cells after RASAL2, NENF or combined RASAL2 and NENF depletion. The level of phosphorylation is expressed as a percentage …
View article: Data from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Data from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
Purpose: We utilized whole-genome mapping of promoters that are activated by DNA hypomethylation in hepatocellular carcinoma (HCC) clinical samples to shortlist novel targets for anticancer therapeutics. We provide a proof of principle of …
View article: Supplementary Figure 8 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Figure 8 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 685KB, Supplementary Figure S8. Expression of different RASAL2 transcript variants in liver cancer cells and normal hepatocytes. (A,B) Expression of RASAL2 as measured by QPCR using primers specific to transcript variant 1 and s…
View article: Supplementary Materials and Methods, Figure Legends, Table Legends from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Materials and Methods, Figure Legends, Table Legends from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 116KB
View article: Supplementary Materials and Methods, Figure Legends, Table Legends from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Materials and Methods, Figure Legends, Table Legends from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 116KB
View article: Supplementary Figure 4 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Figure 4 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 651KB, Supplementary Figure S4. Expression of RASAL2 and NENF on the protein level as determined by western blot using anti-RASAL2 (Bethyl Laboratories Inc., A302-109A) or anti-NENF (Abcam, ab74474) antibody at 1:1000 and 1:500 …
View article: Supplementary Figure 2 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Figure 2 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 492KB, Supplementary Figure S2. (A) Effect of 5-azaCdR at 1.0 microM concentration on non-transformed NorHep cell growth throughout 20 day-treatment. NorHep cells were seeded at a density of 3 x 105 per a 10-cm tissue culture di…
View article: Supplementary Figure 4 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Figure 4 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 651KB, Supplementary Figure S4. Expression of RASAL2 and NENF on the protein level as determined by western blot using anti-RASAL2 (Bethyl Laboratories Inc., A302-109A) or anti-NENF (Abcam, ab74474) antibody at 1:1000 and 1:500 …
View article: Data from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Data from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
Purpose: We utilized whole-genome mapping of promoters that are activated by DNA hypomethylation in hepatocellular carcinoma (HCC) clinical samples to shortlist novel targets for anticancer therapeutics. We provide a proof of principle of …
View article: Supplementary Figure 1 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Figure 1 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 961KB, Supplementary Figure S1. (A,B) Extensive methylation analysis of RASAL2 (A) and NENF (B) promoters in cells treated with 5-azaCdR, different cell lines and patients. Average methylation state of CpG sites was determined b…
View article: Supplementary Table 2 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Table 2 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 71KB, Supplementary Table S2. A list of kinases whose phosphorylation was examined in SkHep1 liver cancer cells after RASAL2, NENF or combined RASAL2 and NENF depletion. The level of phosphorylation is expressed as a percentage …
View article: Supplementary Figure 6 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Figure 6 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 701KB, Supplementary Figure S6. (A) 1) Expression of the depleted genes, RASAL2 and NENF, quantified by QPCR after first (I) and second (II) transfection of Hep3B HCC cell line with scrambled siRNA (siCtrl) and siRNA directed to…
View article: Supplementary Figure 1 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Figure 1 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 961KB, Supplementary Figure S1. (A,B) Extensive methylation analysis of RASAL2 (A) and NENF (B) promoters in cells treated with 5-azaCdR, different cell lines and patients. Average methylation state of CpG sites was determined b…
View article: Supplementary Figure 7 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Figure 7 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 690KB, Supplementary Figure S7. Impact of RASAL2 and NENF depletion on on expression of genes of DNA methylation machinery, DNMT1, MBD2, DNMT3A, and DNMT3B. DNMT1 (A), MBD2 (B), DNMT3A (C), and DNMT3B (D) expression levels quant…
View article: Supplementary Figure 3 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets
Supplementary Figure 3 from Genome-Wide Study of Hypomethylated and Induced Genes in Patients with Liver Cancer Unravels Novel Anticancer Targets Open
PDF file - 774KB, Supplementary Figure S3. RASAL2 and NENF are overexpressed in different types of human cancers. RASAL2 or NENF depletion decreases cancer cell growth and invasive capacities in liver cancer. Changes defined using differen…