Muhammad Saad Yousuf
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View article: A Reference Atlas of the Human Dorsal Root Ganglion
A Reference Atlas of the Human Dorsal Root Ganglion Open
Somatosensory perception largely emerges from diverse peripheral sensory neurons whose cell bodies reside in dorsal root ganglia (DRG). Damage or dysfunction of DRG neurons is a major cause of chronic pain and sensory loss. In mice, deep s…
View article: A molecular map of the human spinal dorsal and ventral horn defines arrangement of neuronal types and glial sex differences
A molecular map of the human spinal dorsal and ventral horn defines arrangement of neuronal types and glial sex differences Open
The spinal cord is the gateway for sensory information from the body as it ascends to the brain, as well as a major motor output center of the nervous system. It is also a key location for sensory-motor integration, and a processing site f…
View article: Academic Resource Utilization and IT Gadget Accessibility Among Undergraduate Students in Baltistan
Academic Resource Utilization and IT Gadget Accessibility Among Undergraduate Students in Baltistan Open
This mixed-methods study investigates academic resource utilization patterns, IT accessibility, and library service satisfaction among undergraduate students studying in higher education institutions of Baltistan region. Data were collecte…
View article: Enabling wider access to human molecular neuroscience research in pain: A simple preservation method for human dorsal root ganglion neurons in Hibernate A media
Enabling wider access to human molecular neuroscience research in pain: A simple preservation method for human dorsal root ganglion neurons in Hibernate A media Open
Summary The use of human dorsal root ganglion (DRG) from organ donors opens the door for research into the molecular biology and physiology of human nociceptors; however, there are barriers to working with this tissue including logistical …
View article: Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use
Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use Open
Loss of GABAergic and glycinergic inhibitory efficacy in the spinal dorsal horn is associated with neuropathic pain and opioid-induced hyperalgesia in rodent models. Downregulation of the KCC2 chloride extrusion transporter is a key mechan…
View article: Frequency of Fast Track Extubation and Factors Affecting its Success in Adult Cardiac Surgery Patients: A Retrospective Analysis
Frequency of Fast Track Extubation and Factors Affecting its Success in Adult Cardiac Surgery Patients: A Retrospective Analysis Open
Objective: The primary objective is to identify how often do adult patients, who undergo elective open-heart surgeries are fast track extubated. Secondary objectives are to identify the causes of delayed extubation and to quantify the freq…
View article: Spatial transcriptomic profiling of human paravertebral sympathetic chain ganglia reveals diabetes-induced neuroplasticity
Spatial transcriptomic profiling of human paravertebral sympathetic chain ganglia reveals diabetes-induced neuroplasticity Open
The paravertebral sympathetic chain ganglia (SCG) are autonomic ganglia critical for regulating the “fight-or-flight” response. Symptoms of sympathetic dysfunction are prevalent in diabetes, affecting up to 90% of patients. The molecular a…
View article: Neurobiological and Chemical Characterization of the Cyanobacterial Metabolite Veraguamide E
Neurobiological and Chemical Characterization of the Cyanobacterial Metabolite Veraguamide E Open
Summary Ver E’s structure was validated by ¹H NMR, HRMS, and molecular networking analyses. Computational docking and NMR titration confirmed direct, saturable, and tight binding of Ver E to the human Sigma-2 receptor/transmembrane protein…
View article: Sigma-2 Receptor/Transmembrane Protein 97 in Pain Modulation: Tracing Historical Roots and Current Research Directions
Sigma-2 Receptor/Transmembrane Protein 97 in Pain Modulation: Tracing Historical Roots and Current Research Directions Open
The sigma-2 receptor/transmembrane protein 97 (σ2R/TMEM97) has emerged as a promising target for treating various diseases, including chronic pain. This review explores the potential of σ2R/TMEM97 as a novel therapeut…
View article: EFFICACY OF ENDOVASCULAR MECHANOCHEMICAL ABLATION (MOCA) WITH FLEBOGRIF TO TREAT VARICOSE VEINS OF LOWER EXTREMITIES: A SINGLE CENTER EXPERIENCE AT SHAHEED MOHTARMA BENAZIR BHUTTO INSTITUTE OF TRAUMA (SMBBIT)
EFFICACY OF ENDOVASCULAR MECHANOCHEMICAL ABLATION (MOCA) WITH FLEBOGRIF TO TREAT VARICOSE VEINS OF LOWER EXTREMITIES: A SINGLE CENTER EXPERIENCE AT SHAHEED MOHTARMA BENAZIR BHUTTO INSTITUTE OF TRAUMA (SMBBIT) Open
Objective: The study aims to determine the outcomes of mechanochemical ablation (MOCA) with flebogrif to treat varicose veins of the lower extremities. Methods: After the ethical approval from institutional review, this prospective observa…
View article: Expansion of OSMR expression and signaling in the human dorsal root ganglion links OSM to neuropathic pain
Expansion of OSMR expression and signaling in the human dorsal root ganglion links OSM to neuropathic pain Open
RNA sequencing studies on human dorsal root ganglion (hDRG) from patients suffering from neuropathic pain show upregulation of OSM, linking this IL-6 family cytokine to pain disorders. In mice, however, OSM signaling causes itch behaviors …
View article: Diroximel fumarate acts through Nrf2 to attenuate methylglyoxal-induced nociception in mice and decrease ISR activation in DRG neurons
Diroximel fumarate acts through Nrf2 to attenuate methylglyoxal-induced nociception in mice and decrease ISR activation in DRG neurons Open
Diabetic neuropathic pain is associated with elevated plasma levels of methylglyoxal (MGO). MGO is a metabolite of glycolysis that causes pain hypersensitivity in mice by stimulating the phosphorylation of eukaryotic initiation factor 2α (…
View article: <b>Diroximel fumarate acts through Nrf2 to attenuate methylglyoxal-induced nociception in mice and decrease ISR activation in DRG neurons</b>
<b>Diroximel fumarate acts through Nrf2 to attenuate methylglyoxal-induced nociception in mice and decrease ISR activation in DRG neurons</b> Open
Diabetic neuropathic pain is associated with elevated plasma levels of methylglyoxal (MGO). MGO is a metabolite of glycolysis that causes pain hypersensitivity in mice by stimulating the phosphorylation of eukaryotic initiation factor 2α (…
View article: Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use
Decreased KCC2 expression in the human spinal dorsal horn associated with chronic pain and long-term opioid use Open
Loss of GABAergic and glycinergic inhibitory efficacy in the spinal dorsal horn is associated with neuropathic pain and opioid-induced hyperalgesia in rodent models. Downregulation of the KCC2 chloride extrusion transporter is a key mechan…
View article: Enabling Wider Access to Human Molecular Neuroscience Research in Pain: A Simple Preservation Method for Human Dorsal Root Ganglion Neurons in Hibernate a Media
Enabling Wider Access to Human Molecular Neuroscience Research in Pain: A Simple Preservation Method for Human Dorsal Root Ganglion Neurons in Hibernate a Media Open
View article: Effect of Intravenous Paracetamol on Postoperative Recovery in Children Undergoing Hypospadias Repair under General Anaesthesia with Caudal Block: A Randomised Controlled Trial
Effect of Intravenous Paracetamol on Postoperative Recovery in Children Undergoing Hypospadias Repair under General Anaesthesia with Caudal Block: A Randomised Controlled Trial Open
Intravenous paracetamol, Caudal analgesia, Ropivacaine, Paediatric patients.
View article: <i>IGHG4</i>Expression in C2 Human Dorsal Root Ganglion Potentially Links B Cells to Spreading Chronic Neck Pain
<i>IGHG4</i>Expression in C2 Human Dorsal Root Ganglion Potentially Links B Cells to Spreading Chronic Neck Pain Open
Very little is known about the molecular mechanisms underlying chronic neck pain, a highly prevalent and burdensome condition. We analyzed the C2 dorsal root ganglion (DRG) of patients with neck pain who underwent C1-2 arthrodesis surgery.…
View article: Post-Neutralisation Activated Clotting Time and Postoperative Transfusions in Cardiac Surgery Outcome
Post-Neutralisation Activated Clotting Time and Postoperative Transfusions in Cardiac Surgery Outcome Open
Coronary Artery bypass grafting, Cardiopulmonary bypass, Activated clotting time (ACT), Heparin, Postoperative bleeding, Blood transfusions.
View article: Anaesthetic and Procedural-Related Complications in Transcatheter Aortic Valve Implantation (TAVI) and Its Outcome: A Retrospective Observational Study
Anaesthetic and Procedural-Related Complications in Transcatheter Aortic Valve Implantation (TAVI) and Its Outcome: A Retrospective Observational Study Open
View article: Dissociating and culturing human dorsal root ganglia neurons v1
Dissociating and culturing human dorsal root ganglia neurons v1 Open
A protocol for culturing and maintaining human dorsal root ganglia (DRG) neurons.
View article: Single-cell RNA sequencing from human dorsal root ganglion v2
Single-cell RNA sequencing from human dorsal root ganglion v2 Open
This protocol describes how to extract single cells from dorsal root ganglia sourced from human organ donors and perform single-cell RNA sequencing using the Single Cell Gene Expression FLEX kit from 10X Genomics.
View article: Challenges and strategies regarding anaesthetic management of twin pregnancy undergoing redo aortic valve replacement
Challenges and strategies regarding anaesthetic management of twin pregnancy undergoing redo aortic valve replacement Open
A multidisciplinary approach is crucial for managing twin pregnancy following redo aortic valve surgery. Careful planning, close monitoring, and specialised surgical and anaesthetic techniques are key to minimising risks to both mother and…
View article: Single stage cricoid split laryngoplasty with costochondral rib grafting is a novel approach to treat subglottic stenosis in a paediatric patient: A case report
Single stage cricoid split laryngoplasty with costochondral rib grafting is a novel approach to treat subglottic stenosis in a paediatric patient: A case report Open
View article: Loss of Sigma-2 Receptor/TMEM97 Is Associated with Neuropathic Injury-Induced Depression-Like Behaviors in Female Mice
Loss of Sigma-2 Receptor/TMEM97 Is Associated with Neuropathic Injury-Induced Depression-Like Behaviors in Female Mice Open
Previous studies have shown that ligands that bind to sigma-2 receptor/TMEM97 (s 2 R/TMEM97), a transmembrane protein, have anxiolytic/antidepressant-like properties and relieve neuropathic pain-like effects in rodents. Despite medical int…
View article: Human Ganglia and Spinal Cord Tissue Procurement from Organ Donors and Tissue Quality Assessment v1
Human Ganglia and Spinal Cord Tissue Procurement from Organ Donors and Tissue Quality Assessment v1 Open
Purpose Describe the human nervous tissue removal, preservation, storage, and transportation procedures during tissue procurement from organ donors. Responsibility The Human Tissue Procurement and Processing core at the University of Texas…
View article: Ephrin-B2 promotes nociceptive plasticity and hyperalgesic priming through EphB2-MNK-eIF4E signaling in both mice and humans
Ephrin-B2 promotes nociceptive plasticity and hyperalgesic priming through EphB2-MNK-eIF4E signaling in both mice and humans Open
Ephrin-B-EphB signaling promotes pain through signaling between dorsal root ganglion (DRG) neurons and spinal cord neurons in the dorsal horn, and through signaling between peripheral cells and EphB receptors expressed by DRG neurons. Prev…
View article: A Comparison of Techniques of Internal Jugular Vein Cannulation: Anatomical Landmark, Ultrasound Guided Pre-location, and Real-Time Ultrasound Guided
A Comparison of Techniques of Internal Jugular Vein Cannulation: Anatomical Landmark, Ultrasound Guided Pre-location, and Real-Time Ultrasound Guided Open
View article: Nerve detection and visualization using hyperspectral imaging for surgical guidance
Nerve detection and visualization using hyperspectral imaging for surgical guidance Open
During surgery of delicate regions, differentiation between nerve and surrounding tissue is crucial. Hyperspectral imaging (HSI) techniques can enhance the contrast between types of tissue beyond what the human eye can differentiate. Where…
View article: Ephrin-B2 Promotes Nociceptive Plasticity and Hyperalgesic Priming Through Ephb2-Mnk-Eif4e Signaling in Both Mice and Humans
Ephrin-B2 Promotes Nociceptive Plasticity and Hyperalgesic Priming Through Ephb2-Mnk-Eif4e Signaling in Both Mice and Humans Open
View article: Diroximel fumarate acts through Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons
Diroximel fumarate acts through Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons Open
Diabetic neuropathic pain is associated with elevated plasma levels of methylglyoxal (MGO). MGO is a metabolite of glycolysis that causes mechanical hypersensitivity in mice by inducing the integrated stress response (ISR), which is charac…