N.K. Boardman
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Effects of pH and Oxygen on Photosynthetic Reactions of Intact Chloroplasts Open
Oxygen inhibition of photosynthesis was studied with intact spinach (Spinacia oleracea L.) chloroplasts which exhibited very high rates of photosynthetic CO(2) reduction and were insensitive to additions of photosynthetic intermediates whe…
Light-dependent absorption and selective scattering changes at 518 nm in chloroplast thylakoid membranes. Open
The light-induced absorbance change at 518 nm of isolated chloroplasts consists of a rapid phase, and a slow phase which is complete in about 20 sec. The slow component of the 518 nm absorbance change correlates with the light-induced chan…
Properties of Protochlorophyllide and Chlorophyll(ide) Holochromes from Etiolated and Greening Leaves Open
Protochlorophyllide and chlorophyll(ide) holochromes (Pchl-H and Chl-H) were extracted from dark-grown and greening seedlings with saponin and partly purified by ammonium sulfate fractionation. Sephadex gel filtration in the presence of sa…
Lipid biosynthesis by isolated plastids from greening pea, Pisum sativum Open
Isolated etioplasts from 8-day-old dark-grown pea seedlings incorporated [1-(14)C]acetate into lipid at a relatively low rate. Plastids from seedlings that had been illuminated for at least 2 hr showed an enhanced incorporation provided th…
Development of Photochemical Activity and the Appearance of the High Potential Form of Cytochrome <i>b</i>-559 in Greening Barley Seedlings Open
The development of photochemical activity during the greening of dark-grown barley seedlings (Hordeum vulgare L. cv. Svalöfs Bonus) was studied in relation to the formation of the high potential form of cytochrome b-559 (cytochrome b-559(H…
Action Spectrum of Cytochrome <i>f</i> Photooxidation in Greening Bean Leaves Open
Photooxidation of cytochrome f can be demonstrated in an etiolated bean leaf after 2 to 3 hr of illumination (3, 4).
Lipid Composition of Pea and Bean Leaves during Chloroplast Development Open
The changes in composition of the complex lipids were followed during the greening of dark-grown pea (Pisum sativum) and bean (Phaseolus vulgaris) seedlings. No significant changes in glycerolipid concentrations in the leaves were observed…
Deficient Photosystem II in Agranal Bundle Sheath Chloroplasts of C <sub>4</sub> Plants Open
A method is described for separating mesophyll and bundle sheath chloroplasts from the leaves of C 4 plants. The agranal bundle sheath chloroplasts are inactive in the Hill reaction, whereas granal bundle sheath and granal mesophyll chloro…
Fat Metabolism in Higher Plants Open
Spinach chloroplasts with intact outer envelopes (Class I) rapidly synthesize oleic and palmitic acids from 14C-acetate in the presence of coenzyme A, sodium bicarbonate, magnesium ion, and light. Light intensity studies reveal a 2-fold st…
Photosynthetic Studies on a Pea-mutant Deficient in Chlorophyll Open
A chlorophyll-deficient mutant of pea (Pisum sativum) was found as a spontaneous mutation of the variety Greenfeast. Total chlorophyll of the mutant leaves was about one-half that of normal pea leaves per mg dry weight, and the ratio of ch…
Studies on The Greening of Dark-Grown Bean Plants II. Development of Photochemical Activity Open
A study of the photochemical activity of plastids isolated from etiolated bean leaves which had been illuminated for increasing periods was undertaken. Proplastids isolated from etiolated leaves were inactive in the Hill reaction. Photoche…
Studies on The Greening of Dark-Grown Bean Plants I. Formation of Chloroplasts From Proplastids Open
Bean plants were grown in the dark for 10-14 days and then transferred to continuous artificial light for varying periods. After illumination, the plastids were isolated and purified by differential and density-gradient centrifugation. The…
Separation of neutral proteins on ion-exchange resins Open
Research Article| April 01 1955 Separation of neutral proteins on ion-exchange resins N. K. Boardman; N. K. Boardman * 1Low Temperature Station for Research in Biochemistry and Biophysics, University of Cambridge, and Department of Scienti…