Paul D. N. Hebert
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View article: DNA barcoding of more than one million insect specimens indicates that at least one third of California's insect biodiversity remains undiscovered
DNA barcoding of more than one million insect specimens indicates that at least one third of California's insect biodiversity remains undiscovered Open
View article: A low-cost, scalable solution for automated cutting of entomological labels
A low-cost, scalable solution for automated cutting of entomological labels Open
The digitization and labelling of historic and new specimens is a time-consuming, error-prone process as labels are still often hand-cut. To improve efficiency and consistency, we developed a low-cost solution for high-throughput environme…
View article: Haplodiploidy accelerates mitogenome evolution in insects
Haplodiploidy accelerates mitogenome evolution in insects Open
Rates of mitogenome evolution differ among animal lineages, and this variation has been linked to life history, to ecological traits and—potentially—to the sex-determination system. Insects are a compelling model for examining the latter f…
View article: The brown copper moth, Tridentaforma browncopper: DNA barcoding reveals a second species in the family Tridentaformidae (Lepidoptera, Adeloidea)
The brown copper moth, Tridentaforma browncopper: DNA barcoding reveals a second species in the family Tridentaformidae (Lepidoptera, Adeloidea) Open
A biodiversity monitoring program in south-central British Columbia, Canada, at sites on traditional nłeʔképmx territory near the Highland Valley Copper mine, collected many specimens of an unknown moth. DNA barcode analysis revealed its a…
View article: CanPests V1.0: A reference dataset for arthropod pests of Canada integrating DNA barcodes
CanPests V1.0: A reference dataset for arthropod pests of Canada integrating DNA barcodes Open
Arthropod pest species represent a serious threat to agriculture and forestry. Canada is no exception, with over 1200 recorded pest species. Although a consolidated dataset would benefit research, management, and policy, information on the…
View article: Common to rare transfer learning (CORAL) enables inference and prediction for a quarter million rare Malagasy arthropods
Common to rare transfer learning (CORAL) enables inference and prediction for a quarter million rare Malagasy arthropods Open
DNA-based biodiversity surveys result in massive-scale data, including up to millions of species—of which, most are rare. Making the most of such data for inference and prediction requires modeling approaches that can relate species occurr…
View article: Tachinid Flies (Diptera), Caterpillar Hosts (Lepidoptera) and Their Food Plants, Reared in Área de Conservación Guanacaste (ACG), Northwestern Costa Rica: Documenting Community Structure with the Aid of DNA Barcodes
Tachinid Flies (Diptera), Caterpillar Hosts (Lepidoptera) and Their Food Plants, Reared in Área de Conservación Guanacaste (ACG), Northwestern Costa Rica: Documenting Community Structure with the Aid of DNA Barcodes Open
We describe the trophic relationships of tachinid parasitoid flies that attack exophagous, leaf-eating Lepidoptera caterpillars in Área de Conservación Guanacaste (ACG), northwestern Costa Rica over approximately forty years beginning in 1…
View article: Caterpillar diet breadth in Área de Conservación Guanacaste, a large and diverse Neotropical wildland in northwestern Costa Rica: toxins, silica, aluminum, and sclerophylly
Caterpillar diet breadth in Área de Conservación Guanacaste, a large and diverse Neotropical wildland in northwestern Costa Rica: toxins, silica, aluminum, and sclerophylly Open
Caterpillar–food plant records collected over approximately 38 years in the Area de Conservación Guanacaste (ACG) in northwestern Costa Rica are described and summarized. The data comprise 431,212 individual rearing records, 197,366 of whi…
View article: <scp>BOLDistilled</scp> : Automated Construction of Comprehensive but Compact <scp>DNA</scp> Barcode Reference Libraries
<span>BOLDistilled</span> : Automated Construction of Comprehensive but Compact <span>DNA</span> Barcode Reference Libraries Open
Advances in DNA sequencing technology have stimulated the rapid uptake of protocols—such as eDNA analysis and metabarcoding—that infer the species composition of environmental samples from DNA sequences. DNA barcode reference libraries pla…
View article: CanPests V1.0: A comprehensive dataset for arthropod pests of Canada integrating DNA barcodes
CanPests V1.0: A comprehensive dataset for arthropod pests of Canada integrating DNA barcodes Open
Arthropod pest species represent a serious threat to agriculture and forestry. Canada is no exception, with over 1200 recorded pest species. Although a consolidated dataset would benefit research, management, and policy, information on the…
View article: Farmland Biodiversity Monitoring Using DNA Metabarcoding
Farmland Biodiversity Monitoring Using DNA Metabarcoding Open
Although 5–20% of global crop production is lost to arthropod damage, current biomonitoring programs are extremely limited. This study evaluates the feasibility of using metabarcoding to assess overall insect diversity and detect pest spec…
View article: Farmland Biodiversity Monitoring Using DNA Metabarcoding
Farmland Biodiversity Monitoring Using DNA Metabarcoding Open
Although 5-20% of global crop production is lost to arthropod damage, current biomonitoring programs are extremely limited. This study evaluates the feasibility of using metabarcoding to assess overall insect diversity and detect pest spec…
View article: Metabarcoding arthropods in agroecosystems in Southern Ontario, Canada
Metabarcoding arthropods in agroecosystems in Southern Ontario, Canada Open
Metabarcoding can generate large numbers of georeferenced occurrence data from bulk samples at low cost. Its integration into the practice of agricultural invertebrate biomonitoring currently lacks both standard methods and example dataset…
View article: Incidence and attributes of chimeric COI and 18S sequences derived from nematode-infected arthropods
Incidence and attributes of chimeric COI and 18S sequences derived from nematode-infected arthropods Open
High-throughput sequencing is speeding the discovery of new species and making it possible to quantify the species richness of bulk samples. However, chimeric sequences can lead to both type I errors (true species mistakenly rejected) and …
View article: Metabarcoding arthropods in agroecosystems in Southern Ontario, Canada
Metabarcoding arthropods in agroecosystems in Southern Ontario, Canada Open
Metabarcoding can generate large numbers of georeferenced occurrence data from bulk samples at low cost. Its integration into the practice of agricultural invertebrate biomonitoring currently lacks both standard methods and example dataset…
View article: Automating the Curation of <scp>DNA</scp> Barcode Databases for Vascular Plants
Automating the Curation of <span>DNA</span> Barcode Databases for Vascular Plants Open
Comprehensive, curated, and current DNA barcode reference databases are essential for both the identification of single specimens and for the interpretation of metabarcoding data. In the case of plants, nuclear (ITS) and plastid (rbcL, mat…
View article: Automating the Curation of DNA Barcode Databases for Vascular Plants
Automating the Curation of DNA Barcode Databases for Vascular Plants Open
Comprehensive, curated, and current DNA barcode reference databases are essential for both the identification of single specimens and for the interpretation of metabarcoding data. In the case of plants, nuclear (ITS) and plastid (rbcL, mat…
View article: Extensive DNA Barcoding of Lepidoptera of Crete (Greece) Reveals Significant Taxonomic and Faunistic Gaps and Supports the First Comprehensive Checklist of the Island’s Fauna
Extensive DNA Barcoding of Lepidoptera of Crete (Greece) Reveals Significant Taxonomic and Faunistic Gaps and Supports the First Comprehensive Checklist of the Island’s Fauna Open
Comprehensive genetic surveys of Lepidoptera are still largely lacking across most of the eastern Mediterranean. Consequently, there is a lack of modern, taxonomically validated checklists that meet current scientific standards. In this st…
View article: Multiple variants of the mitochondrial COI DNA barcode region are prevalent in North European sawflies
Multiple variants of the mitochondrial COI DNA barcode region are prevalent in North European sawflies Open
DNA barcoding, the use of standard segments of DNA to assign specimens to a species, has emerged as a major field of biodiversity research over the last 20 years. Large-scale global initiatives are building DNA barcode reference libraries …
View article: BOLDistilled: Comprehensive but compact DNA barcode reference libraries
BOLDistilled: Comprehensive but compact DNA barcode reference libraries Open
Advances in DNA sequencing technology have stimulated the rapid uptake of protocols—such as eDNA analysis and metabarcoding—that infer the species composition of environmental samples from DNA sequences. DNA barcode reference libraries pla…
View article: Extensive DNA Barcoding of Lepidoptera of Crete (Greece) Reveals Significant Taxonomic and Faunistic Gaps and Supports the First Comprehensive Checklist of the Island’s Fauna
Extensive DNA Barcoding of Lepidoptera of Crete (Greece) Reveals Significant Taxonomic and Faunistic Gaps and Supports the First Comprehensive Checklist of the Island’s Fauna Open
Comprehensive genetic surveys of Lepidoptera are still largely lacking across most of the eastern Mediterranean. Consequently, there is a lack of modern, taxonomically validated checklists that meet current scientific standards. In this st…
View article: Aquatic Biotas of Sundaland Are Fragmented But Not Refugial
Aquatic Biotas of Sundaland Are Fragmented But Not Refugial Open
Tropical insular systems have long attracted biologists, stimulating some important controversies in ecology and evolution. Eustatic fluctuations during the Pleistocene have been invoked to explain species dispersal and proliferation in th…
View article: Data on insect biodiversity in a Chinese potato agroecosystem from DNA metabarcoding
Data on insect biodiversity in a Chinese potato agroecosystem from DNA metabarcoding Open
Potato ( Solanum tuberosum ) is a staple crop important in global food security. As a leading potato producer, China faces significant challenges from insect pest infestations that compromise yield and quality. However, insect communities …
View article: Professor Jeremy Nichol McNeil, a retrospective
Professor Jeremy Nichol McNeil, a retrospective Open
View article: Correction: Spatial and seasonal determinants of arthropod community composition across an agro-ecosystem landscape
Correction: Spatial and seasonal determinants of arthropod community composition across an agro-ecosystem landscape Open
View article: HAPLODIPLOIDY ACCELERATES MITOGENOME EVOLUTION IN INSECTS
HAPLODIPLOIDY ACCELERATES MITOGENOME EVOLUTION IN INSECTS Open
Rates of mitogenome evolution differ among animal lineages, variation which has been linked to life history, ecological traits, and potentially to breeding system. Insects are a good model for examining the latter impacts as, although most…
View article: Forty-Five Years of Caterpillar Rearing in Area de Conservación Guanacaste (ACG) Northwestern Costa Rica: DNA Barcodes, BINs, and a First Description of Plant–Caterpillar–Ichneumonoid Interactions Detected
Forty-Five Years of Caterpillar Rearing in Area de Conservación Guanacaste (ACG) Northwestern Costa Rica: DNA Barcodes, BINs, and a First Description of Plant–Caterpillar–Ichneumonoid Interactions Detected Open
Foliage-feeding wild caterpillars have been collected and reared year-round by 1–30 rural resident parataxonomists in the Area de Conservación Guanacaste (ACG) in northwestern Costa Rica since 1978. The aim of the work was to describe the …
View article: Towards a Taxonomy Machine: A Training Set of 5.6 Million Arthropod Images
Towards a Taxonomy Machine: A Training Set of 5.6 Million Arthropod Images Open
The taxonomic identification of organisms from images is an active research area within the machine learning community. Current algorithms are very effective for object recognition and discrimination, but they require extensive training da…
View article: Barcode 100K Specimens: In a Single Nanopore Run
Barcode 100K Specimens: In a Single Nanopore Run Open
It is a global priority to better manage the biosphere, but action must be informed by comprehensive data on the abundance and distribution of species. The acquisition of such information is currently constrained by high costs. DNA barcodi…
View article: Response of parasitoid communities to insecticide application during a <i>Lymantria dispar</i> outbreak in mixed oak forests
Response of parasitoid communities to insecticide application during a <i>Lymantria dispar</i> outbreak in mixed oak forests Open
In the temperate mixed oak forests of Central Europe, outbreaks of insects such as the spongy moth, Lymantria dispar , can cause severe defoliation and insecticide is sometimes applied for their control. Parasitoids, mainly Hymenoptera and…