Daphne N. Dorst
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View article: CD8+ T cells drive myofibroblast activation and contraction via JAK/STAT3 and TGFβ signaling
CD8+ T cells drive myofibroblast activation and contraction via JAK/STAT3 and TGFβ signaling Open
Fibrosis in rheumatic connective tissue diseases is marked by CD8+ T cell and pro-fibrotic myofibroblast infiltration, though the role of CD8+ T cells in myofibroblast activity remains unexplored. To address this, we developed a 3D cell cu…
View article: Depleting Autoreactive B-Cells Using Targeted Photodynamic Therapy
Depleting Autoreactive B-Cells Using Targeted Photodynamic Therapy Open
In many autoimmune pathologies, including Rheumatoid Arthritis (RA), only a small percentage of the total B cell population is autoreactive and sustain disease. Yet, current immunotherapy treatments often eliminate the entire B-cell popula…
View article: Depleting autoreactive B-cells using targeted photodynamic therapy
Depleting autoreactive B-cells using targeted photodynamic therapy Open
In many autoimmune pathologies, including Rheumatoid Arthritis (RA), only a small percentage of the total B cell population is autoreactive and sustain disease. Yet, current immunotherapy treatments often eliminate the entire B-cell popula…
View article: CD8+ T cells drive myofibroblast activation and contraction via JAK/STAT3 and TGFβ signaling
CD8+ T cells drive myofibroblast activation and contraction via JAK/STAT3 and TGFβ signaling Open
Background: Fibrosis is a major cause of morbidity and mortality in rheumatic connective tissue diseases. In these conditions, fibrotic tissues are characterized by the infiltration of CD8+ T cells and pro-fibrotic myofibroblasts. However,…
View article: Fibroblast Activation Protein-Targeted Photodynamic Therapy of Cancer-Associated Fibroblasts in Murine Models for Pancreatic Ductal Adenocarcinoma
Fibroblast Activation Protein-Targeted Photodynamic Therapy of Cancer-Associated Fibroblasts in Murine Models for Pancreatic Ductal Adenocarcinoma Open
Patients with pancreatic ductal adenocarcinoma (PDAC) have a dismal 5 year survival of 9%. One important limiting factor for treatment efficacy is the dense tumor-supporting stroma. The cancer-associated fibroblasts in this stroma deposit …
View article: Fibroblast Activation Protein-Targeting Minibody-IRDye700DX for Ablation of the Cancer-Associated Fibroblast with Photodynamic Therapy
Fibroblast Activation Protein-Targeting Minibody-IRDye700DX for Ablation of the Cancer-Associated Fibroblast with Photodynamic Therapy Open
Fibroblast activation protein (FAP), expressed on cancer-associated fibroblasts, is a target for diagnosis and therapy in multiple tumour types. Strategies to systemically deplete FAP-expressing cells show efficacy; however, these induce t…
View article: A Novel In Vitro Disease Model for Systemic Sclerosis Using Polyisocyanide Hydrogels
A Novel In Vitro Disease Model for Systemic Sclerosis Using Polyisocyanide Hydrogels Open
Systemic sclerosis (SSc) is a rare autoimmune disease with limited treatment options that is characterized by fibrosis in various organs. To screen the effectiveness of new therapies, there is an urgent need for reliable in vitro models. K…
View article: Fibroblast Activation Protein Targeted Photodynamic Therapy Selectively Kills Activated Skin Fibroblasts from Systemic Sclerosis Patients and Prevents Tissue Contraction
Fibroblast Activation Protein Targeted Photodynamic Therapy Selectively Kills Activated Skin Fibroblasts from Systemic Sclerosis Patients and Prevents Tissue Contraction Open
Systemic sclerosis (SSc) is a rare, severe, auto-immune disease characterized by inflammation, vasculopathy and fibrosis. Activated (myo)fibroblasts are crucial drivers of this fibrosis. By exploiting their expression of fibroblast activat…
View article: Photodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development
Photodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development Open
Macrophages play a crucial role in the initiation and progression of rheumatoid arthritis (RA). Liposomes can be used to deliver therapeutics to macrophages by exploiting their phagocytic ability. However, since macrophages serve as the im…
View article: Targeting of fibroblast activation protein in rheumatoid arthritis patients: imaging and <i>ex vivo</i> photodynamic therapy
Targeting of fibroblast activation protein in rheumatoid arthritis patients: imaging and <i>ex vivo</i> photodynamic therapy Open
Objective Activated synovial fibroblasts are key effector cells in RA. Selectively depleting these based upon their expression of fibroblast activation protein (FAP) is an attractive therapeutic approach. Here we introduce FAP imaging of i…
View article: Targeted photodynamic therapy selectively kills activated fibroblasts in experimental arthritis
Targeted photodynamic therapy selectively kills activated fibroblasts in experimental arthritis Open
Objective In RA, synovial fibroblasts become activated. These cells express fibroblast activation protein (FAP) and contribute to the pathogenesis by producing cytokines, chemokines and proteases. Selective depletion in inflamed joints cou…
View article: Endotoxemia shifts neutrophils with TIMP-free gelatinase B/MMP-9 from bone marrow to the periphery and induces systematic upregulation of TIMP-1
Endotoxemia shifts neutrophils with TIMP-free gelatinase B/MMP-9 from bone marrow to the periphery and induces systematic upregulation of TIMP-1 Open
Lipopolysaccharides or endotoxins elicit an excessive host inflammatory response and lead to life-threatening conditions such as endotoxemia and septic shock. Lipopolysaccharides trigger mobilization and stimulation of leukocytes and exagg…