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View article: De Novo transcriptome assembly of Diaprepes abbreviatus: foundations for functional genomics and targeted pest management
De Novo transcriptome assembly of Diaprepes abbreviatus: foundations for functional genomics and targeted pest management Open
We present a high-quality de novo transcriptome assembly of D. abbreviatus with detailed, stage-specific transcript coverage. The identification of key developmentally regulated genes offers valuable insights into the molecular mechanisms …
View article: Identification of CAP genes in finger lime (Citrus australasica) and their role in plant responses to abiotic and biotic stress
Identification of CAP genes in finger lime (Citrus australasica) and their role in plant responses to abiotic and biotic stress Open
The study focuses on the in silico analysis of cysteine-rich secretory proteins and PR1-like (CAP) genes in finger lime (Citrus australasica), a citrus species known for its tolerance to Huanglongbing (HLB). We identified several PR1-like …
View article: Photosynthesis Responses to the Infection with Plant Pathogens
Photosynthesis Responses to the Infection with Plant Pathogens Open
Photosynthesis, the remarkable process by which green plants synthesize nutrients using light energy, plays a crucial role in sustaining life on Earth. However, the effects of pathogens on photosynthesis are not widely understood. In gener…
View article: Virus-induced gene silencing simultaneously exploits ‘attract and kill’ traits in plants and insects to manage huanglongbing
Virus-induced gene silencing simultaneously exploits ‘attract and kill’ traits in plants and insects to manage huanglongbing Open
The vector-borne disease huanglongbing (HLB) causes severe economic losses to citrus production worldwide with no available cure. Herein, we applied virus-induced gene silencing technology to engineer citrus that preferentially attracted a…
View article: Epigenetic modulation of fungal pathogens: a focus on Magnaporthe oryzae
Epigenetic modulation of fungal pathogens: a focus on Magnaporthe oryzae Open
Epigenetics has emerged as a potent field of study for understanding the factors influencing the effectiveness of human disease treatments and for identifying alternations induced by pathogens in host plants. However, there has been a pauc…
View article: Melatonin supplementation enhances browning suppression and improves transformation efficiency and regeneration of transgenic rough lemon plants (Citrus × jambhiri)
Melatonin supplementation enhances browning suppression and improves transformation efficiency and regeneration of transgenic rough lemon plants (Citrus × jambhiri) Open
Enzymatic browning poses a significant challenge that limits in vitro propagation and genetic transformation of plant tissues. This research focuses on investigating how adding antioxidant substances can suppress browning, leading to impro…
View article: Influence of Anthocyanin Expression on the Performance of Photosynthesis in Sweet Orange, Citrus sinensis (L.) Osbeck
Influence of Anthocyanin Expression on the Performance of Photosynthesis in Sweet Orange, Citrus sinensis (L.) Osbeck Open
Anthocyanins are a class of natural pigments that accumulate transiently or permanently in plant tissues, often in response to abiotic and biotic stresses. They play a photoprotective role by attenuating the irradiance incident on the phot…
View article: Physiological and Biochemical Evaluation of Salt Stress Tolerance in a Citrus Tetraploid Somatic Hybrid
Physiological and Biochemical Evaluation of Salt Stress Tolerance in a Citrus Tetraploid Somatic Hybrid Open
Somatic hybridization has emerged as a valuable tool for developing novel genetic combinations in citrus breeding programs, including the creation of salt-tolerant rootstocks. In this study, the performance of a tetraploid somatic hybrid, …
View article: Gamma-Aminobutyric Acid Accumulation Contributes to Citrus sinensis Response against ‘Candidatus Liberibacter Asiaticus’ via Modulation of Multiple Metabolic Pathways and Redox Status
Gamma-Aminobutyric Acid Accumulation Contributes to Citrus sinensis Response against ‘Candidatus Liberibacter Asiaticus’ via Modulation of Multiple Metabolic Pathways and Redox Status Open
Huanglongbing (HLB; also known as citrus greening) is the most destructive bacterial disease of citrus worldwide with no known sustainable cure yet. Herein, we used non-targeted metabolomics and transcriptomics to prove that γ-aminobutyric…
View article: Gamma-Aminobutyric Acid Supplementation Boosts the Phytohormonal Profile in ‘Candidatus Liberibacter asiaticus’-Infected Citrus
Gamma-Aminobutyric Acid Supplementation Boosts the Phytohormonal Profile in ‘Candidatus Liberibacter asiaticus’-Infected Citrus Open
The devastating citrus disease, Huanglongbing (HLB), is associated with ‘Candidatus Liberibacter sp.’ and transmitted by citrus psyllids. Unfortunately, HLB has no known sustainable cure yet. Herein, we proposed γ-aminobutyric acid (GABA) …
View article: In Silico Characterization and Gene Expression Analysis of Toll Signaling Pathway-Related Genes in Diaphorina citri
In Silico Characterization and Gene Expression Analysis of Toll Signaling Pathway-Related Genes in Diaphorina citri Open
The Asian citrus psyllid, Diaphorina citri is the main vector of citrus greening disease, also known as Huanglongbing (HLB). Currently, mitigating HLB depends on the control of D. citri using insecticides. To design innovative control stra…
View article: Systemic Uptake of Oxytetracycline and Streptomycin in Huanglongbing-Affected Citrus Groves after Foliar Application and Trunk Injection
Systemic Uptake of Oxytetracycline and Streptomycin in Huanglongbing-Affected Citrus Groves after Foliar Application and Trunk Injection Open
Huanglongbing (HLB), which is caused by the phloem-limited bacterium ‘Candidatus Liberibacter asiaticus,’ is an economically important disease of citrus in many regions of the world. Due to the significant damage caused by the HLB disease …
View article: Using X-ray Micro-Computed Tomography to Three-Dimensionally Visualize the Foregut of the Glassy-Winged Sharpshooter (Homalodisca vitripennis)
Using X-ray Micro-Computed Tomography to Three-Dimensionally Visualize the Foregut of the Glassy-Winged Sharpshooter (Homalodisca vitripennis) Open
Dissecting the heads of small insects belonging to the order Hemiptera for detailed anatomical investigation with light or scanning electron microscopy is difficult, time-consuming, and destructive, often resulting in sample preparation ar…
View article: Better together: the use of virus-induced gene silencing technique to repress the expression of two endogenous citrus genes simultaneously
Better together: the use of virus-induced gene silencing technique to repress the expression of two endogenous citrus genes simultaneously Open
Virus-induced gene silencing is a promising technique for functional genomics studies. Citrus tristeza virus was employed successfully to create an infectious clone that was used to silence endogenous citrus genes. Phytoene desaturase (PDS…
View article: Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1
Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1 Open
The curry leaf tree, Bergera koenigii , is highly attractive to the Asian citrus psyllid, Diaphorina citri , which vectors the bacterial causative agent of citrus greening or huanglongbing disease. This disease has decimated citrus product…
View article: Silencing Phytoene Desaturase Causes Alteration in Monoterpene Volatiles Belonging to the Methylerythritol Phosphate Pathway
Silencing Phytoene Desaturase Causes Alteration in Monoterpene Volatiles Belonging to the Methylerythritol Phosphate Pathway Open
Volatile organic compounds (VOCs) are a large group of lipophilic hydrocarbon compounds derived from different biosynthetic pathways in plants. VOCs are produced and released from plants as a defense mechanism against biotic and abiotic st…
View article: Silencing of δ‐aminolevulinic acid dehydratase via virus induced gene silencing promotes callose deposition in plant phloem
Silencing of δ‐aminolevulinic acid dehydratase via virus induced gene silencing promotes callose deposition in plant phloem Open
The δ-aminolevulinic acid dehydratase (ALAD) enzyme is an intermediate in the biosynthetic pathway of tetrapyrroles. It combines two δ-aminolevulinic acid (δ-ALA) molecules to form the pyrrole, porphobilinogen, an important precursor for p…
View article: Not Just a Cycle: Three <i>gab</i> Genes Enable the Non-Cyclic Flux Toward Succinate via GABA Shunt in ‘<i>Candidatus</i> Liberibacter asiaticus’–Infected Citrus
Not Just a Cycle: Three <i>gab</i> Genes Enable the Non-Cyclic Flux Toward Succinate via GABA Shunt in ‘<i>Candidatus</i> Liberibacter asiaticus’–Infected Citrus Open
Although the mitochondria retain all required enzymes for an intact tricarboxylic acid (TCA) cycle, plants might shift the cyclic flux from the TCA cycle to an alternative noncyclic pathway via γ-aminobutyric acid (GABA) shunt under specif…
View article: Influence of Rootstock on the Leaf Volatile Organic Compounds of Citrus Scion Is More Pronounced after the Infestation with Diaphorina citri
Influence of Rootstock on the Leaf Volatile Organic Compounds of Citrus Scion Is More Pronounced after the Infestation with Diaphorina citri Open
Nowadays, citrus greening or Huanglongbing is considered the most destructive disease in the citrus industry worldwide. In the Americas and Asia, the disease is caused by the putative pathogen, ‘Candidatus Liberibacter asiaticus’ and trans…
View article: Metabolomic analysis elucidates how shade conditions ameliorate the deleterious effects of greening (Huanglongbing) disease in citrus
Metabolomic analysis elucidates how shade conditions ameliorate the deleterious effects of greening (Huanglongbing) disease in citrus Open
Summary Under tropical and subtropical environments, citrus leaves are exposed to excess sunlight, inducing photoinhibition. Huanglongbing (HLB, citrus greening), a devastating phloem‐limited disease putatively caused by Candidatus Liberib…
View article: The Chorion Proteome of Diaphorina citri, the Vector of Huanglongbing Disease in Citrus
The Chorion Proteome of Diaphorina citri, the Vector of Huanglongbing Disease in Citrus Open
Nowadays, the Asian citrus psyllid, Diaphorina citri (Kuwayama) (Hemiptera: Liviidae) is considered the most devastating pest of citrus because it transmits “Candidatus Liberibacter asiaticus”, the putative causal agent of huanglongbing (H…
View article: Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Liviidae)
Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Liviidae) Open
The Asian citrus psyllid, Diaphorina citri Kuwayama is devastating the citrus industry worldwide. It transmits ‘Candidatus Liberibacter asiaticus’, the pathogen of Huanglongbing in citrus. RNA interference is an excellent tool for function…
View article: Detection of Oxytetracycline in Citrus Phloem and Xylem Saps Using Europium-Based Method
Detection of Oxytetracycline in Citrus Phloem and Xylem Saps Using Europium-Based Method Open
Oxytetracycline (OTC) has been used for the control of several plant diseases and was recently approved for the control of Huanglongbing, the citrus greening disease. Huanglongbing is caused by the phloem limited ‘Candidatus Liberibacter a…
View article: Citrate Mediated Europium-Based Detection of Oxytetracycline in Citrus Tissues
Citrate Mediated Europium-Based Detection of Oxytetracycline in Citrus Tissues Open
Oxytetracycline (OTC) and streptomycin have been used for the control of several plant diseases and were recently permitted for the control of citrus greening disease, Huanglongbing. Consequently, sensitive and reliable methods are highly …
View article: Diaphorina citri Genome Possesses a Complete Melatonin Biosynthesis Pathway Differentially Expressed under the Influence of the Phytopathogenic Bacterium, Candidatus Liberibacter asiaticus
Diaphorina citri Genome Possesses a Complete Melatonin Biosynthesis Pathway Differentially Expressed under the Influence of the Phytopathogenic Bacterium, Candidatus Liberibacter asiaticus Open
Melatonin is synthesized from the amino acid L-tryptophan via the shikimic acid pathway and ubiquitously distributed in both prokaryotes and eukaryotes. Although most of melatonin biosynthesis genes were characterized in several plants and…