Naglaa M. Hagag
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View article: Genomic surveillance and evolution of co-circulating avian influenza H5N1 and H5N8 viruses in Egypt, 2022–2024
Genomic surveillance and evolution of co-circulating avian influenza H5N1 and H5N8 viruses in Egypt, 2022–2024 Open
For over two decades, avian influenza virus (AIV) has significantly impacted the Egyptian poultry population, with multiple subtypes and genotypes contributing to significant economic and agricultural losses. As part of an ongoing national…
View article: Isolation, characterization and phylogenetic analyses of avian influenza A (H9N2) viruses isolated from poultry between 2019 and 2023 in Egypt
Isolation, characterization and phylogenetic analyses of avian influenza A (H9N2) viruses isolated from poultry between 2019 and 2023 in Egypt Open
The current study aimed to investigate the genetic characterization and evolution of low pathogenic avian influenza virus H9N2 in Egypt. Ten H9N2 viruses were recently isolated from samples collected between 2019 and 2023. Phylogenetic ana…
View article: A novel immunoinformatic approach for design and evaluation of heptavalent multiepitope foot-and-mouth disease virus vaccine
A novel immunoinformatic approach for design and evaluation of heptavalent multiepitope foot-and-mouth disease virus vaccine Open
Background Foot-and-mouth disease virus (FMDV) vaccine development can be a laborious task due to the existence of various serotypes and lineages and its quasi-species nature. Immunoinformatics provide effective and promising avenue for th…
View article: Estimation of Heritability, Genetic and Phenotypic Correlation of Head and Neck Traits of Egyptian Arabian Colts
Estimation of Heritability, Genetic and Phenotypic Correlation of Head and Neck Traits of Egyptian Arabian Colts Open
Morphometric characteristics of the head and neck are distinctive breed features in Egyptian Arabian horses, yet their heritability patterns remain incompletely characterized. This study investigated ten morphometric traits in 91 purebred …
View article: A Novel Application of Virus Like Particles in the Hemagglutination Inhibition Assay
A Novel Application of Virus Like Particles in the Hemagglutination Inhibition Assay Open
The hemagglutination inhibition (HI) assay is a traditional laboratory procedure for detection and quantitation of serum antibodies of hemagglutinating viruses containing the hemagglutinin (HA) gene. The current study aimed to investigate …
View article: Advancing IBDV diagnostics: a one-step multiplex real-time qRT-PCR for discriminating between vvIBDV and non-vvIBDV viruses, including the newly emerged IBDV variant
Advancing IBDV diagnostics: a one-step multiplex real-time qRT-PCR for discriminating between vvIBDV and non-vvIBDV viruses, including the newly emerged IBDV variant Open
The very virulent infectious bursal disease virus (vvIBDV) induces an acute, highly contagious and immunosuppressive disease in younger chicken causing massive economic losses globally. A major challenge in the field’s clinical diagnosis i…
View article: A case of a twin surviving to term following the abortion of its co-twin at 9 months in an Arabian mare
A case of a twin surviving to term following the abortion of its co-twin at 9 months in an Arabian mare Open
Twin pregnancy in mares is one of the leading causes of abortions. Abortion invariably impacts both fetuses. This report describes an unusual case of a twin surviving to term following the abortion of its co-twin at 9 months in a 7-year-ol…
View article: Emergence of the Novel Infectious bursal disease viruse Variant in Vaccinated Poultry Flocks in Egypt
Emergence of the Novel Infectious bursal disease viruse Variant in Vaccinated Poultry Flocks in Egypt Open
Infectious bursal disease viruses (IBDVs) have a profound impact on poultry production worldwide, directly causing mortality rates of up to 100%, and indirectly through their immunosuppressive effects. Since the emergence of the antigenica…
View article: Immunogenicity and Cross-Protective Efficacy Induced by an Inactivated Recombinant Avian Influenza A/H5N1 (Clade 2.3.4.4b) Vaccine against Co-Circulating Influenza A/H5Nx Viruses
Immunogenicity and Cross-Protective Efficacy Induced by an Inactivated Recombinant Avian Influenza A/H5N1 (Clade 2.3.4.4b) Vaccine against Co-Circulating Influenza A/H5Nx Viruses Open
Controlling avian influenza viruses (AIVs) is mainly based on culling of the infected bird flocks or via the implementation of inactivated vaccines in countries where AIVs are considered to be endemic. Over the last decade, several avian i…
View article: Interspecies transmission of SARS CoV-2 with special emphasis on viral mutations and ACE-2 receptor homology roles
Interspecies transmission of SARS CoV-2 with special emphasis on viral mutations and ACE-2 receptor homology roles Open
COVID-19 outbreak was first reported in 2019, Wuhan, China. The spillover of the disease caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), to a wide range of pet, zoo, wild, and farm animals has emphasized potential z…
View article: Circulating foot-and-mouth disease virus serotype A African-genotype IV in Egypt during 2022
Circulating foot-and-mouth disease virus serotype A African-genotype IV in Egypt during 2022 Open
Background and Aim: Foot-and-mouth disease (FMD) virus causes continuous outbreaks, leading to serious economic consequences that affect animal productivity and restrict trade movement. The potential influence of the disease was due to the…
View article: Development of multiplex gold nanoparticles biosensors for ultrasensitive detection and genotyping of equine herpes viruses
Development of multiplex gold nanoparticles biosensors for ultrasensitive detection and genotyping of equine herpes viruses Open
Gold nanoparticles (GNPs) biosensors can detect low viral loads and differentiate between viruses types, enabling early diagnosis and effective disease management. In the present study, we developed GNPs biosensors with two different cappi…
View article: First record of abortion of single co-twin in an Arabian mare and continuity of the other to full term and birth
First record of abortion of single co-twin in an Arabian mare and continuity of the other to full term and birth Open
Twin pregnancies in Arabian horses is a major cause of abortion. In all cases when the abortion occurs, it occurs for both fetuses. Unusually in the case described here, which we believe has not previously been documented in Arabian horses…
View article: Emergence of Highly Pathogenic Avian Influenza A Virus (H5N1) of Clade 2.3.4.4b in Egypt, 2021–2022
Emergence of Highly Pathogenic Avian Influenza A Virus (H5N1) of Clade 2.3.4.4b in Egypt, 2021–2022 Open
Wild migratory birds have the capability to spread avian influenza virus (AIV) over long distances as well as transmit the virus to domestic birds. In this study, swab and tissue samples were obtained from 190 migratory birds during close …
View article: Feline Pan Leukopenia Molecular Detection and Viral Phylogeny in Egypt
Feline Pan Leukopenia Molecular Detection and Viral Phylogeny in Egypt Open
Feline panleukopenia virus (FPV) causes severe disease in cats, as well as in a variety of wild carnivores including many endangered animals.Regular appearance of the virus increases the importance of evaluating the percentage of identity …
View article: SARS‐CoV‐2 infection of companion animals in Egypt and its risk of spillover
SARS‐CoV‐2 infection of companion animals in Egypt and its risk of spillover Open
Background Reverse zoonoses occur because of interactions between humans and animals. Homology of ACE‐2 cell receptors in different hosts and high mutation rate of SARS‐CoV‐2 enhance viral transmission among species. Objectives This study …
View article: Circulating Mutant of Foot and Mouth Disease virus serotype A-African- Genotype IV in Egypt during 2022
Circulating Mutant of Foot and Mouth Disease virus serotype A-African- Genotype IV in Egypt during 2022 Open
Foot and Mouth Disease Virus causes continuously annoying outbreaks and massive animal illnesses. Usually, the potential influence of the disease was due to the emergence of conquered emergent new strains or re-emergence of local strains w…
View article: Molecular Epidemiology and Evolutionary Analysis of Avian Influenza A(H5) Viruses Circulating in Egypt, 2019–2021
Molecular Epidemiology and Evolutionary Analysis of Avian Influenza A(H5) Viruses Circulating in Egypt, 2019–2021 Open
The highly pathogenic avian influenza (HPAI) H5N8 virus was first detected in Egypt in late 2016. Since then, the virus has spread rapidly among different poultry sectors, becoming the dominant HPAI H5 subtype reported in Egypt. Different …
View article: Molecular Identification of Virulence Genes of Pathogenic Escherichia coli Isolated from Broilers Chicken
Molecular Identification of Virulence Genes of Pathogenic Escherichia coli Isolated from Broilers Chicken Open
This study was implemented to isolate, characterize the presence of E coli and study their antibiotic resistance and virulence genes in broiler chickens in Assiut city .A total of 120 samples (liver, heart, yolk sac and lung) were gained f…
View article: Mutations of the SARS-CoV-2 Spike Glycoprotein Detected in Cats and Their Effect on Its Structure and Function
Mutations of the SARS-CoV-2 Spike Glycoprotein Detected in Cats and Their Effect on Its Structure and Function Open
The high frequency of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) mutations and homology of the Angiotensin-Converting Enzyme-2 (ACE2) cell receptors in various hosts help the virus transcend species barriers. In this stud…
View article: Genetic Variations among Different Variants of G1-like Avian Influenza H9N2 Viruses and Their Pathogenicity in Chickens
Genetic Variations among Different Variants of G1-like Avian Influenza H9N2 Viruses and Their Pathogenicity in Chickens Open
Since it was first discovered, the low pathogenic avian influenza (LPAI) H9N2 subtype has established linages infecting the poultry population globally and has become one of the most prevalent influenza subtypes in domestic poultry. Severa…
View article: Application of Loop-Mediated Isothermal Amplification (LAMP) in Sex Identification of Parrots Bred in Egypt
Application of Loop-Mediated Isothermal Amplification (LAMP) in Sex Identification of Parrots Bred in Egypt Open
Over 400 of the 3800 tropical avian species are endangered or threatened. One of many solutions to conserve animal biodiversity is breeding animals in zoos or private animal farms. Animal breeding programs are difficult to implement in spe…