Nancy Gerloff
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View article: Tracking poliovirus through wastewater: environmental surveillance insights from Haïti (2020–2023)
Tracking poliovirus through wastewater: environmental surveillance insights from Haïti (2020–2023) Open
Environmental surveillance (ES) for poliovirus is a critical component of global eradication efforts, enabling the detection of virus circulation in communities, even in the absence of acute flaccid paralysis (AFP) cases. To monitor poliov…
View article: Advancing poliovirus eradication: lessons learned from piloting direct molecular detection of polioviruses in high-risk and priority geographies
Advancing poliovirus eradication: lessons learned from piloting direct molecular detection of polioviruses in high-risk and priority geographies Open
In the Global Polio Laboratory Network (GPLN), poliovirus (PV) screening results from acute flaccid paralysis (AFP) surveillance is based on virus isolation (VI) through cell culture, entailing long turnaround times and the amplification o…
View article: Evaluating the Effectiveness of External Molecular Proficiency Testing in the Global Polio Laboratory Network, 2021–2022
Evaluating the Effectiveness of External Molecular Proficiency Testing in the Global Polio Laboratory Network, 2021–2022 Open
In the Global Poliovirus Laboratory Network (GPLN), participation and successful completion in annual proficiency test (PT) panels has been a part of the WHO accreditation process for decades. The PT panel is a molecular external quality a…
View article: Wastewater Surveillance for Poliovirus in Selected Jurisdictions, United States, 2022–2023
Wastewater Surveillance for Poliovirus in Selected Jurisdictions, United States, 2022–2023 Open
Wastewater testing can inform public health action as a component of polio outbreak response. During 2022-2023, a total of 7 US jurisdictions (5 states and 2 cities) participated in prospective or retrospective testing of wastewater for po…
View article: Correction: Validation of a redesigned pan-poliovirus assay and real-time PCR platforms for the global poliovirus laboratory network
Correction: Validation of a redesigned pan-poliovirus assay and real-time PCR platforms for the global poliovirus laboratory network Open
[This corrects the article DOI: 10.1371/journal.pone.0255795.].
View article: Molecular epidemiology of enteroviruses from Guatemalan wastewater isolated from human lung fibroblasts
Molecular epidemiology of enteroviruses from Guatemalan wastewater isolated from human lung fibroblasts Open
The Global Specialized Polio Laboratory at CDC supports the Global Poliovirus Laboratory Network with environmental surveillance (ES) to detect the presence of vaccine strain polioviruses, vaccine-derived polioviruses, and wild polioviruse…
View article: Validation of improved automated nucleic acid extraction methods for direct detection of polioviruses for global polio eradication
Validation of improved automated nucleic acid extraction methods for direct detection of polioviruses for global polio eradication Open
Polioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses of the family Picornaviridae. As we approach polio eradication, accurate and timely detection of poliovirus in sto…
View article: Wastewater Testing and Detection of Poliovirus Type 2 Genetically Linked to Virus Isolated from a Paralytic Polio Case — New York, March 9–October 11, 2022
Wastewater Testing and Detection of Poliovirus Type 2 Genetically Linked to Virus Isolated from a Paralytic Polio Case — New York, March 9–October 11, 2022 Open
In July 2022, a case of paralytic poliomyelitis resulting from infection with vaccine-derived poliovirus (VDPV) type 2 (VDPV2)§ was confirmed in an unvaccinated adult resident of Rockland County, New York (1). As of August 10, 2022, poliov…
View article: Public Health Response to a Case of Paralytic Poliomyelitis in an Unvaccinated Person and Detection of Poliovirus in Wastewater — New York, June–August 2022
Public Health Response to a Case of Paralytic Poliomyelitis in an Unvaccinated Person and Detection of Poliovirus in Wastewater — New York, June–August 2022 Open
On July 18, 2022, the New York State Department of Health (NYSDOH) notified CDC of detection of poliovirus type 2 in stool specimens from an unvaccinated immunocompetent young adult from Rockland County, New York, who was experiencing acut…
View article: Genome Sequences of 16 Enterovirus Isolates from Environmental Sewage in Guatemala, 2019 to 2021
Genome Sequences of 16 Enterovirus Isolates from Environmental Sewage in Guatemala, 2019 to 2021 Open
Enteroviruses can cause human infectious disease. We report 16 near-complete genome sequences of enteroviruses that were isolated through environmental surveillance of wastewater in Guatemala.
View article: CaFÉ: A Sensitive, Low-Cost Filtration Method for Detecting Polioviruses and Other Enteroviruses in Residual Waters
CaFÉ: A Sensitive, Low-Cost Filtration Method for Detecting Polioviruses and Other Enteroviruses in Residual Waters Open
Acute flaccid paralysis (AFP) surveillance has been used to identify polio cases and target vaccination campaigns since the inception of the Global Poliovirus Eradication Initiative (GPEI) in 1988. To date, only Afghanistan and Pakistan ha…
View article: Culture-Independent Detection of Poliovirus in Stool Samples by Direct RNA Extraction
Culture-Independent Detection of Poliovirus in Stool Samples by Direct RNA Extraction Open
The GPLN is a global surveillance system composed of 146 laboratories in 92 countries, in each of the six World Health Organization regions. Laboratory surveillance for PV relies on virus isolation by cell culture to identify PV in stool s…
View article: Direct detection of polioviruses using a recombinant poliovirus receptor
Direct detection of polioviruses using a recombinant poliovirus receptor Open
Polioviruses are positive-sense, single-stranded RNA picornaviruses and the principal cause of poliomyelitis. Global poliovirus surveillance has relied on poliovirus isolation in cells, which may take a minimum of 10 days, involves maintai…
View article: Validation of a redesigned pan-poliovirus assay and real-time PCR platforms for the global poliovirus laboratory network
Validation of a redesigned pan-poliovirus assay and real-time PCR platforms for the global poliovirus laboratory network Open
Surveillance and detection of polioviruses (PV) remain crucial to monitoring eradication progress. Intratypic differentiation (ITD) using the real-time RT-PCR kit is key to the surveillance workflow, where viruses are screened after cell c…
View article: Direct detection of polioviruses using a recombinant poliovirus receptor
Direct detection of polioviruses using a recombinant poliovirus receptor Open
Polioviruses are positive-sense, single-stranded RNA picornaviruses and the principal cause of poliomyelitis. Global poliovirus surveillance has relied on poliovirus isolation in cells, which may take a minimum of 10 days, involves maintai…
View article: Diagnostic Assay Development for Poliovirus Eradication
Diagnostic Assay Development for Poliovirus Eradication Open
With poliovirus eradication nearing, few pockets of active wild poliovirus (WPV) transmission remain in the world. Intratypic differentiation (ITD) plays a crucial part in laboratory surveillance as the molecular detection method that can …
View article: Mild Respiratory Illness Among Young Children Caused by Highly Pathogenic Avian Influenza A (H5N1) Virus Infection in Dhaka, Bangladesh, 2011
Mild Respiratory Illness Among Young Children Caused by Highly Pathogenic Avian Influenza A (H5N1) Virus Infection in Dhaka, Bangladesh, 2011 Open
Identification of H5N1 cases through population-based surveillance suggests possible additional undetected cases throughout Bangladesh and highlights the importance of surveillance for mild respiratory illness among populations frequently …
View article: Shifting Clade Distribution, Reassortment, and Emergence of New Subtypes of Highly Pathogenic Avian Influenza A(H5) Viruses Collected from Vietnamese Poultry from 2012 to 2015
Shifting Clade Distribution, Reassortment, and Emergence of New Subtypes of Highly Pathogenic Avian Influenza A(H5) Viruses Collected from Vietnamese Poultry from 2012 to 2015 Open
Whole-genome sequences of representative highly pathogenic avian influenza A(H5) viruses from Vietnam were generated, comprising samples from poultry outbreaks and active market surveillance collected from January 2012 to August 2015. Six …
View article: Genetically Diverse Low Pathogenicity Avian Influenza A Virus Subtypes Co-Circulate among Poultry in Bangladesh
Genetically Diverse Low Pathogenicity Avian Influenza A Virus Subtypes Co-Circulate among Poultry in Bangladesh Open
Influenza virus surveillance, poultry outbreak investigations and genomic sequencing were assessed to understand the ecology and evolution of low pathogenicity avian influenza (LPAI) A viruses in Bangladesh from 2007 to 2013. We analyzed 5…