Natsuko Miura
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View article: Enhanced deoxyviolacein bioproduction via enzyme condensation with a yeast glycolytic enzyme‐derived peptide tag
Enhanced deoxyviolacein bioproduction via enzyme condensation with a yeast glycolytic enzyme‐derived peptide tag Open
In microbial production, regulating endogenous and exogenous synthetic pathways is essential. Techniques to induce intracellular enzyme condensation have attracted attention as a means to increase apparent enzyme activity with minimal tran…
View article: Characterization of carotenoid-producing<i>Muricauda</i>sp. strains isolated from the reef-building coral<i>Galaxea fascicularis</i>and subtropical seawater
Characterization of carotenoid-producing<i>Muricauda</i>sp. strains isolated from the reef-building coral<i>Galaxea fascicularis</i>and subtropical seawater Open
Bacterial symbionts in corals and coral-associated zooxanthellae are attracting increasing attention as potential probiotics. Some members of the family Flavobacteriaceae are zooxanthellae-associated bacteria, which are known to protect zo…
View article: Deciphering mechanisms of UV filter- and temperature-induced bleaching in the coral<i>Acropora tenuis</i>, using ecotoxicogenomics
Deciphering mechanisms of UV filter- and temperature-induced bleaching in the coral<i>Acropora tenuis</i>, using ecotoxicogenomics Open
Coral reefs are at risk of bleaching due to various environmental and anthropogenic stressors such as global warming and chemical pollutants. However, there is little understanding of stressor-specific mechanisms that cause coral bleaching…
View article: Foci-forming regions of pyruvate kinase and enolase at the molecular surface incorporate proteins into yeast cytoplasmic metabolic enzymes transiently assembling (META) bodies
Foci-forming regions of pyruvate kinase and enolase at the molecular surface incorporate proteins into yeast cytoplasmic metabolic enzymes transiently assembling (META) bodies Open
Spatial reorganization of metabolic enzymes to form the “metabolic enzymes transiently assembling (META) body” is increasingly recognized as a mechanism contributing to regulation of cellular metabolism in response to environmental changes…
View article: Mutualistic Interactions between Dinoflagellates and Pigmented Bacteria Mitigate Environmental Stress
Mutualistic Interactions between Dinoflagellates and Pigmented Bacteria Mitigate Environmental Stress Open
Coral reefs cover less than 0.1% of the ocean floor, but about 25% of all marine species depend on coral reefs at some point in their life cycles. However, rising ocean temperatures associated with global climate change are a serious threa…
View article: Condensate Formation by Metabolic Enzymes in Saccharomyces cerevisiae
Condensate Formation by Metabolic Enzymes in Saccharomyces cerevisiae Open
Condensate formation by a group of metabolic enzymes in the cell is an efficient way of regulating cell metabolism through the formation of “membrane-less organelles.” Because of the use of green fluorescent protein (GFP) for investigating…
View article: Peptide barcoding for one-pot evaluation of sequence–function relationships of nanobodies
Peptide barcoding for one-pot evaluation of sequence–function relationships of nanobodies Open
View article: Simple Esterification of [1-<sup>13</sup>C]-Alpha-Ketoglutarate Enhances Membrane Permeability and Allows for Noninvasive Tracing of Glutamate and Glutamine Production
Simple Esterification of [1-<sup>13</sup>C]-Alpha-Ketoglutarate Enhances Membrane Permeability and Allows for Noninvasive Tracing of Glutamate and Glutamine Production Open
Alpha-ketoglutarate (α-KG) is a key metabolite and signaling molecule in cancer cells, but the low permeability of α-KG limits the study of α-KG mediated effects in vivo. Recently, cell-permeable monoester and diester α-KG derivatives have…
View article: Synthesis of [1‐<sup>13</sup>C‐5‐<sup>12</sup>C]‐alpha‐ketoglutarate enables noninvasive detection of 2‐hydroxyglutarate
Synthesis of [1‐<sup>13</sup>C‐5‐<sup>12</sup>C]‐alpha‐ketoglutarate enables noninvasive detection of 2‐hydroxyglutarate Open
Isocitrate dehydrogenase 1 (IDH1) mutations that generate the oncometabolite 2‐hydroxyglutarate (2‐HG) from α‐ketoglutarate (α‐KG) have been identified in many types of tumors and are an important prognostic factor in gliomas. 2‐HG product…
View article: Generation of two‐dimensional electron gas to normally depleted AlGaN/GaN hetero‐interface by SiO <sub>2</sub> deposition and subsequent high‐temperature annealing
Generation of two‐dimensional electron gas to normally depleted AlGaN/GaN hetero‐interface by SiO <sub>2</sub> deposition and subsequent high‐temperature annealing Open
SiO 2 film deposition and subsequent high‐temperature annealing resulted in the generation of a two‐dimensional electron gas (2DEG) at Al(Ga)N/GaN hetero‐interfaces, of which the 2DEG was originally fully depleted. The obtained mobilities …
View article: An Experimental Study on the Effects of Hot and ColdCompresses on Skin Injuries Caused by ChemotherapyExtravasation
An Experimental Study on the Effects of Hot and ColdCompresses on Skin Injuries Caused by ChemotherapyExtravasation Open
The management of intravenous therapy is an important task for nurses.Extravasation of drugs is an adverse event of infusion therapy that can occurwith a variety of drugs. The frequency of extravasation of anticancer drugsranges from 0.01%…
View article: A Zeaxanthin-Producing Bacterium Isolated from the Algal Phycosphere Protects Coral Endosymbionts from Environmental Stress
A Zeaxanthin-Producing Bacterium Isolated from the Algal Phycosphere Protects Coral Endosymbionts from Environmental Stress Open
Occupying less than 1% of the seas, coral reefs are estimated to harbor ∼25% of all marine species. However, the destruction of coral reefs has intensified in the face of global climate changes, such as rising seawater temperatures, which …
View article: Design of novel primer sets for easy detection of <i>Ruegeria</i> species from seawater
Design of novel primer sets for easy detection of <i>Ruegeria</i> species from seawater Open
Some coral-associated bacteria show protective roles for corals against pathogens. However, the distribution of coral-protecting bacteria in seawater is not well known. In addition, compared with the methods for investigating coral pathoge…
View article: High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics
High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics Open
Since G-protein coupled receptors (GPCRs) are linked to various diseases, screening of functional ligands against GPCRs is vital for drug discovery. In the present study, we developed a high-throughput functional cell-based assay by combin…
View article: Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris
Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris Open
Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies, utilization of microbes is an…
View article: Peptide barcoding for establishment of new types of genotype–phenotype linkages
Peptide barcoding for establishment of new types of genotype–phenotype linkages Open
Measuring binding properties of binders (e.g., antibodies) is essential for developing useful experimental reagents, diagnostics, and pharmaceuticals. Display technologies can evaluate a large number of binders in a high-throughput manner,…
View article: Design of novel primer sets for easy detection of <i>Ruegeria</i> species from seawater
Design of novel primer sets for easy detection of <i>Ruegeria</i> species from seawater Open
Some coral-associated bacteria show protective roles for corals against pathogens. However, the distribution of coral-protecting bacteria in seawater is not well known. In addition, compared with the methods for investigating coral pathoge…
View article: MOESM1 of Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris
MOESM1 of Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris Open
Additional file 1. Additional tables.
View article: Evaluation of Unconventional Protein Secretion by Saccharomyces cerevisiae and other Fungi
Evaluation of Unconventional Protein Secretion by Saccharomyces cerevisiae and other Fungi Open
Development of proteome analysis of extracellular proteins has revealed that a wide variety of proteins, including fungal allergens are present outside the cell. These secreted allergens often do not contain known secretion signal sequence…
View article: Three-dimensional alginate hydrogels for radiobiological and metabolic studies of cancer cells
Three-dimensional alginate hydrogels for radiobiological and metabolic studies of cancer cells Open
View article: Alginate Hydrogels for Three-Dimensional Culture and Real-Time Monitoring of Cancer Cell Metabolism and Radiation Response
Alginate Hydrogels for Three-Dimensional Culture and Real-Time Monitoring of Cancer Cell Metabolism and Radiation Response Open
View article: Validation of defect size to be detected during isi based on probabilistic fracture mechanics
Validation of defect size to be detected during isi based on probabilistic fracture mechanics Open
View article: Prognostic significance of gene amplification of ACTN4 in stage I and II oral tongue cancer
Prognostic significance of gene amplification of ACTN4 in stage I and II oral tongue cancer Open
Despite complete resection of the early stage of oral tongue cancer by partial glossectomy, late cervical lymph node metastasis is frequently observed. Gene amplification of ACTN4 (protein name: actinin-4) is closely associated with the me…
View article: Reconstruction of thermotolerant yeast by one-point mutation identified through whole-genome analyses of adaptively-evolved strains
Reconstruction of thermotolerant yeast by one-point mutation identified through whole-genome analyses of adaptively-evolved strains Open
Saccharomyces cerevisiae is used as a host strain in bioproduction, because of its rapid growth, ease of genetic manipulation, and high reducing capacity. However, the heat produced during the fermentation processes inhibits the biological…
View article: Effect of Differential DNA Repair Process Inhibition on Radiosensitization
Effect of Differential DNA Repair Process Inhibition on Radiosensitization Open
View article: ACTN4 copy number increase as a predictive biomarker for chemoradiotherapy of locally advanced pancreatic cancer
ACTN4 copy number increase as a predictive biomarker for chemoradiotherapy of locally advanced pancreatic cancer Open
The copy number of ACTN4 is a predictive biomarker for CRT of LAPC.