Nathan Curry
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View article: High content 3D imaging by dual-view oblique plane microscopy
High content 3D imaging by dual-view oblique plane microscopy Open
Oblique plane microscopy (OPM) is a form of light-sheet fluorescence microscopy (LSFM) employing a single microscope objective at the sample for both fluorescence excitation and detection. Dual-view OPM (dOPM) is an optically folded form o…
View article: Geometric deep learning and multiple-instance learning for 3D cell-shape profiling
Geometric deep learning and multiple-instance learning for 3D cell-shape profiling Open
The three-dimensional (3D) morphology of cells emerges from complex cellular and environmental interactions, serving as an indicator of cell state and function. In this study, we used deep learning to discover morphology representations an…
View article: Connexin 30 locally controls actin cytoskeleton and mechanical remodeling in motile astrocytes
Connexin 30 locally controls actin cytoskeleton and mechanical remodeling in motile astrocytes Open
During brain maturation, astrocytes establish complex morphologies unveiling intense structural plasticity. Connexin 30 (Cx30), a gap‐junction channel‐forming protein expressed postnatally, dynamically regulates during development astrocyt…
View article: Environmentally dependent and independent control of 3D cell shape
Environmentally dependent and independent control of 3D cell shape Open
View article: 3D single-cell shape analysis using geometric deep learning
3D single-cell shape analysis using geometric deep learning Open
A bstract Aberrations in 3D cell morphogenesis are linked to diseases such as cancer. Yet there is little systems-level understanding of cell shape determination in 3D, largely because there is a paucity of data-driven methods to quantify …
View article: Environmentally dependent and independent control of cell shape determination by Rho GTPase regulators in melanoma
Environmentally dependent and independent control of cell shape determination by Rho GTPase regulators in melanoma Open
In order to invade 3D tissues, cancer cells dynamically change cell morphology in response to geometric and mechanical cues in the environment. But how cells determine their shape in 3D versus 2D environments is poorly understood. Studying…
View article: In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with autism risk genes
In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with autism risk genes Open
An in vivo analysis of autism risk genes CRISPR targeting in vivo, especially in mammals, can be difficult and time consuming when attempting to determine the effects of a single gene. However, such studies may be required to identify path…
View article: Oblique plane microscopy-based 3D plate-reader investigation of control of cell shape by Rho-regulatory proteins in distinct microenvironments
Oblique plane microscopy-based 3D plate-reader investigation of control of cell shape by Rho-regulatory proteins in distinct microenvironments Open
View article: Multidimensional spectroscopy and imaging of defects in synthetic diamond: excitation-emission-lifetime luminescence measurements with multiexponential fitting and phasor analysis
Multidimensional spectroscopy and imaging of defects in synthetic diamond: excitation-emission-lifetime luminescence measurements with multiexponential fitting and phasor analysis Open
We report the application of phasor analysis and nonlinear iterative fitting to complex spatial and spectroscopic luminescence decay data obtained from multidimensional microscopy of a CVD diamond grown on a HPHT substrate. This spectral a…
View article: FIN-Seq: transcriptional profiling of specific cell types from frozen archived tissue of the human central nervous system
FIN-Seq: transcriptional profiling of specific cell types from frozen archived tissue of the human central nervous system Open
Thousands of frozen, archived tissue samples from the human central nervous system (CNS) are currently available in brain banks. As recent developments in RNA sequencing technologies are beginning to elucidate the cellular diversity presen…
View article: <i>In vivo</i> Perturb-Seq reveals neuronal and glial abnormalities associated with Autism risk genes
<i>In vivo</i> Perturb-Seq reveals neuronal and glial abnormalities associated with Autism risk genes Open
The thousands of disease risk genes and loci identified through human genetic studies far outstrip our current capacity to systematically study their functions. New experimental approaches are needed for functional investigations of large …
View article: Fast Fluorescence Lifetime Imaging Reveals the Aggregation Processes of α-Synuclein and Polyglutamine in Aging <i>Caenorhabditis elegans</i>
Fast Fluorescence Lifetime Imaging Reveals the Aggregation Processes of α-Synuclein and Polyglutamine in Aging <i>Caenorhabditis elegans</i> Open
The nematode worm Caenorhabditis elegans has emerged as an important model organism in the study of the molecular mechanisms of protein misfolding diseases associated with amyloid formation because of its small size, ease of genetic manipu…
View article: Neuronal Activity Drives Astroglial Connexin 30 in Perisynaptic Processes and Shapes Its Functions
Neuronal Activity Drives Astroglial Connexin 30 in Perisynaptic Processes and Shapes Its Functions Open
Astrocytes play key roles in brain functions through dynamic interactions with neurons. One of their typical features is to express high levels of connexins (Cxs), Cx43 and Cx30, the gap junction (GJ)-forming proteins. Cx30 is involved in …
View article: FIN-Seq: Transcriptional profiling of specific cell types in frozen archived tissue from the human central nervous system
FIN-Seq: Transcriptional profiling of specific cell types in frozen archived tissue from the human central nervous system Open
Thousands of frozen, archived tissues from postmortem human central nervous system (CNS) are currently available in brain banks. As single cell and single nucleus technologies are beginning to elucidate the cellular diversity present withi…
View article: Calcium binding at the C-terminus of α-synuclein modulates synaptic vesicle interaction
Calcium binding at the C-terminus of α-synuclein modulates synaptic vesicle interaction Open
View article: C-terminal calcium binding of α-synuclein modulates synaptic vesicle interaction
C-terminal calcium binding of α-synuclein modulates synaptic vesicle interaction Open
View article: Fast Fluorescence Lifetime Imaging for Longitudinal Studies of Protein Aggregation in Living C. Elegans
Fast Fluorescence Lifetime Imaging for Longitudinal Studies of Protein Aggregation in Living C. Elegans Open
View article: 2-Photon Lithography for Nanofluidic Lab-on-Chip Devices
2-Photon Lithography for Nanofluidic Lab-on-Chip Devices Open
View article: Simultaneous AFM and FLIM Imaging with a SiR-DNA Probe Reveals Structural Changes during DNA Condensation in Live Cell Nuclei
Simultaneous AFM and FLIM Imaging with a SiR-DNA Probe Reveals Structural Changes during DNA Condensation in Live Cell Nuclei Open
View article: Live Cell STED-AFM Analysis Correlates Cytoskeletal Structure Remodelling and Membrane Physical Properties during Polarized Migration in Astrocytes
Live Cell STED-AFM Analysis Correlates Cytoskeletal Structure Remodelling and Membrane Physical Properties during Polarized Migration in Astrocytes Open
View article: Development and application of correlative STED and AFM to investigate neuronal cells
Development and application of correlative STED and AFM to investigate neuronal cells Open
Over the past three decades in cellular neuroscience there has been a shift towards the view of the ``tripartite synapse'', where, astrocytes -- as well as the pre-synapse and post-synapse -- are involved in synaptic signalling. The migrat…
View article: Correlative STED and Atomic Force Microscopy on Live Astrocytes Reveals Plasticity of Cytoskeletal Structure and Membrane Physical Properties during Polarized Migration
Correlative STED and Atomic Force Microscopy on Live Astrocytes Reveals Plasticity of Cytoskeletal Structure and Membrane Physical Properties during Polarized Migration Open
The plasticity of the cytoskeleton architecture and membrane properties is important for the establishment of cell polarity, adhesion and migration. Here, we present a method which combines stimulated emission depletion (STED) super-resolu…
View article: Gene network and familial analyses uncover a gene network involving Tbx5/Osr1/Pcsk6 interaction in the second heart field for atrial septation
Gene network and familial analyses uncover a gene network involving Tbx5/Osr1/Pcsk6 interaction in the second heart field for atrial septation Open
Atrial septal defects (ASDs) are a common human congenital heart disease (CHD) that can be induced by genetic abnormalities. Our previous studies have demonstrated a genetic interaction between Tbx5 and Osr1 in the second heart field (SHF)…