Nathan G. Hendricks
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View article: Blood to Biomarker Quantitation in Under One Hour with Rapid Proteomics using a Hyperthermoacidic Protease
Blood to Biomarker Quantitation in Under One Hour with Rapid Proteomics using a Hyperthermoacidic Protease Open
Multi-step multi-hour tryptic proteolysis has limited the utility of bottom-up proteomics for cases that require immediate quantitative information. The recently available hyperthermoacidic (HTA) protease “Krakatoa” digests samples in a si…
View article: An inflection point in high-throughput proteomics with Orbitrap Astral: analysis of biofluids, cells, and tissues
An inflection point in high-throughput proteomics with Orbitrap Astral: analysis of biofluids, cells, and tissues Open
This technical note presents a comprehensive proteomics workflow for the new combination of Orbitrap and Astral mass analyzers across biofluids, cells, and tissues. Central to our workflow is the integration of Adaptive Focused Acoustics (…
View article: The tomato chloroplast stromal proteome compendium elucidated by leveraging a plastid protein-localization prediction Atlas
The tomato chloroplast stromal proteome compendium elucidated by leveraging a plastid protein-localization prediction Atlas Open
Tomato ( Solanum lycopersicum ) is a model species for studying fruit development, wounding, herbivory, and pathogen attack. Despite tomato’s world-wide economic importance and the role of chloroplasts as metabolic hubs and integrators of …
View article: Proteomes of Micro- and Nanosized Carriers Engineered from Red Blood Cells
Proteomes of Micro- and Nanosized Carriers Engineered from Red Blood Cells Open
Red blood cell (RBC)-derived systems offer a potential platform for delivery of biomedical cargos. Although the importance of specific proteins associated with the biodistribution and pharmacokinetics of these particles has been recognized…
View article: Impact of Protein Nitration on Influenza Virus Infectivity and Immunogenicity
Impact of Protein Nitration on Influenza Virus Infectivity and Immunogenicity Open
Nitric oxide and peroxynitrite produced during microbial infections have diverse and seemingly paradoxical functions. While nitration of lung tissue during influenza virus infection has been observed in both mice and humans, the direct eff…
View article: Isoform-dependent lysosomal degradation and internalization of apolipoprotein E requires autophagy proteins
Isoform-dependent lysosomal degradation and internalization of apolipoprotein E requires autophagy proteins Open
The human apolipoprotein E4 isoform (APOE4) is the strongest genetic risk factor for late-onset Alzheimer's disease (AD), and lysosomal dysfunction has been implicated in AD pathogenesis. We found, by examining cells stably expressing each…
View article: High-Resolution Mapping of Amino Acid Residues in DNA–Protein Cross-Links Enabled by Ribonucleotide-Containing DNA
High-Resolution Mapping of Amino Acid Residues in DNA–Protein Cross-Links Enabled by Ribonucleotide-Containing DNA Open
DNA-protein cross-links have broad applications in mapping DNA-protein interactions and provide structural insights into macromolecular structures. However, high-resolution mapping of DNA-interacting amino acid residues with tandem mass sp…
View article: Synthesis of new S S and C C bonds by photoinitiated radical recombination reactions in the gas phase
Synthesis of new S S and C C bonds by photoinitiated radical recombination reactions in the gas phase Open
View article: Production of packed nanospray emitters with integrated emitter-end frits for liquid-chromatography mass spectrometry
Production of packed nanospray emitters with integrated emitter-end frits for liquid-chromatography mass spectrometry Open
This protocol describes how to frit the emitter-end of a pulled silica capillary and subsequently pack it to produce a robust integrated column.Integrating liquid chromatography separations directly into the emitter of a nanospray source f…
View article: Dissociative Energy Transfer for Probing Structural Details of Biomolecules in the Gas-Phase
Dissociative Energy Transfer for Probing Structural Details of Biomolecules in the Gas-Phase Open
The structure of biomolecules is of fundamental importance to their function and activity. Proteins and peptides adopt three-dimensional conformations that affect every facet of their role in biology ranging from stability, solubility, cat…
View article: Two-step energy transfer enables use of phenylalanine in action-EET for distance constraint determination in gaseous biomolecules
Two-step energy transfer enables use of phenylalanine in action-EET for distance constraint determination in gaseous biomolecules Open
Two-step energy transfer is observed between phenylalanine, tyrosine, and modified cysteine. This gas-phase system enables use of phenylalanine in energy transfer experiments, provides specific distance information for structure determinat…