Nele A Haelterman
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View article: Identification of sensory fiber types in mouse temporomandibular joint tissues
Identification of sensory fiber types in mouse temporomandibular joint tissues Open
View article: KneEZ Clear, an Effective Tissue Clearing Protocol to Study Musculoskeletal Tissues in the Mouse
KneEZ Clear, an Effective Tissue Clearing Protocol to Study Musculoskeletal Tissues in the Mouse Open
Wholemount, 3-dimensional (3D) tissue imaging holds significant promise for analyzing heterogeneous musculoskeletal tissues, such as knee joints, that demand time- and labor-intensive processing using traditional histological methods. Curr…
View article: Optimization of Tissue Clearing Methods and Imaging Conditions for 3D Visualization of the Vasculature of the Adult Murine Knee
Optimization of Tissue Clearing Methods and Imaging Conditions for 3D Visualization of the Vasculature of the Adult Murine Knee Open
The vasculature is an essential regulator of tissue oxygenation, metabolism, and immune surveillance under developmental and homeostatic conditions, as well as in regeneration and disease. Within the musculoskeletal system, blood vessels a…
View article: Focal periphyseal edema (FOPE) revisited: does injury severity predict presence and frequency of FOPE lesions?
Focal periphyseal edema (FOPE) revisited: does injury severity predict presence and frequency of FOPE lesions? Open
Background Focal periphyseal edema (FOPE) is a MRI finding identified in long bones in children nearing skeletal maturity. Existing literature is conflicting, with the majority suggesting that FOPEs are potential pain generators related to…
View article: A guide to developing harmonized research workflows in a team science context
A guide to developing harmonized research workflows in a team science context Open
View article: Nuclei Isolation on Murine DRG for Single Nucleus RNA Sequencing v1
Nuclei Isolation on Murine DRG for Single Nucleus RNA Sequencing v1 Open
This is a fast and reproducible method for nuclei isolation on murine dorsal root ganglia for downstream single nuclei RNA sequencing. This protocol utilizes the Nuclei Extraction Buffer, Anti-Nucleus Microbeads, gentleMACS Octo Dissociato…
View article: Optimization of Tissue Clearing Methods and Imaging Conditions for 3D Visualization of the Vasculature of the Adult Murine Knee
Optimization of Tissue Clearing Methods and Imaging Conditions for 3D Visualization of the Vasculature of the Adult Murine Knee Open
View article: Knee histology protocol for immunohistochemistry on cryosections v1
Knee histology protocol for immunohistochemistry on cryosections v1 Open
Osteoarthritis (OA) is a chronic, degenerative condition characterized by whole-joint involvement, affecting the bone, cartilage, synovium, ligaments, and joint capsule. Given its widespread impact on millions of individuals, continued inv…
View article: Histology Protocol for Paraffin Embedded Mouse Knee v1
Histology Protocol for Paraffin Embedded Mouse Knee v1 Open
This method describes how to harvest, embed, and process, mouse knee joints for paraffin-based histological analysis.
View article: KneEZ Clear: an Effective Tissue Clearing Method for Intact Mouse Joints, including the Knee v2
KneEZ Clear: an Effective Tissue Clearing Method for Intact Mouse Joints, including the Knee v2 Open
KneEZ Clear is a modified version of the EZ Clear method (Hsu et al., 2022), optimized for clearing mouse musculoskeletal tissues. KneEZ Clear works well for the clearing of mouse knee and ankle joints, spines, temporomandibular joints, an…
View article: KneEZ Clear: an Effective Tissue Clearing Method for Intact Mouse Joints, including the Knee v1
KneEZ Clear: an Effective Tissue Clearing Method for Intact Mouse Joints, including the Knee v1 Open
KneEZ Clear is a modified version of the EZ Clear method (Hsu et al., 2022), optimized for clearing mouse musculoskeletal tissues. KneEZ Clear works well for the clearing of mouse knee and ankle joints, spines, temporomandibular joints, an…
View article: Editorial: Reproducibility in neuroscience
Editorial: Reproducibility in neuroscience Open
Scientific progress depends on the ability to independently repeat and validate key scientific findings. To verify the rigor, robustness, and validity of a research study, scientists test if they can reach the same conclusions when they us…
View article: Early Career Representative Engagement Task Force: Final Report and Recommendations
Early Career Representative Engagement Task Force: Final Report and Recommendations Open
In 2022, the Federation of American Societies for Experimental Biology (FASEB) convened the Early Career Representative Engagement Task Force, charged with identifying• needs of early-career scientists that can be addressed by scientific s…
View article: Editor's evaluation: Transcriptome-wide association study and eQTL colocalization identify potentially causal genes responsible for human bone mineral density GWAS associations
Editor's evaluation: Transcriptome-wide association study and eQTL colocalization identify potentially causal genes responsible for human bone mineral density GWAS associations Open
View article: Drosophila functional screening of de novo variants in autism uncovers damaging variants and facilitates discovery of rare neurodevelopmental diseases
Drosophila functional screening of de novo variants in autism uncovers damaging variants and facilitates discovery of rare neurodevelopmental diseases Open
View article: A community-led initiative for training in reproducible research
A community-led initiative for training in reproducible research Open
Open and reproducible research practices increase the reusability and impact of scientific research. The reproducibility of research results is influenced by many factors, most of which can be addressed by improved education and training. …
View article: Tendon and motor phenotypes in the Crtap-/- mouse model of recessive osteogenesis imperfecta
Tendon and motor phenotypes in the Crtap-/- mouse model of recessive osteogenesis imperfecta Open
Osteogenesis imperfecta (OI) is characterized by short stature, skeletal deformities, low bone mass, and motor deficits. A subset of OI patients also present with joint hypermobility; however, the role of tendon dysfunction in OI pathogene…
View article: Author response: A community-led initiative for training in reproducible research
Author response: A community-led initiative for training in reproducible research Open
Article Figures and data Abstract Why is training in reproducibility needed? Reproducibility for Everyone (R4E) How can scientists use the R4E materials? Conclusions Data availability References Decision letter Author response Article and …
View article: Author response: Tendon and motor phenotypes in the Crtap-/- mouse model of recessive osteogenesis imperfecta
Author response: Tendon and motor phenotypes in the Crtap-/- mouse model of recessive osteogenesis imperfecta Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Osteogenesis imperfecta (OI) is characteri…
View article: <i>Drosophila</i>functional screening of<i>de novo</i>variants in autism uncovers deleterious variants and facilitates discovery of rare neurodevelopmental diseases
<i>Drosophila</i>functional screening of<i>de novo</i>variants in autism uncovers deleterious variants and facilitates discovery of rare neurodevelopmental diseases Open
Summary Individuals with autism spectrum disorders (ASD) exhibit an increased burden of de novo variants in a broadening range of genes. We functionally tested the effects of ASD missense variants using Drosophila through ‘humanization’ re…
View article: Reproducibility for everyone: a community-led initiative with global reach in reproducible research training
Reproducibility for everyone: a community-led initiative with global reach in reproducible research training Open
Reproducibility is a cornerstone of the scientific method and sets apart science from pseudoscience. Unfortunately, a majority of scientists have experienced difficulties in reproducing their own or someone else’s results. This inability t…
View article: Tendon and Motor Phenotypes in the<i>Crtap<sup>-/-</sup></i>Mouse Model of Recessive Osteogenesis Imperfecta
Tendon and Motor Phenotypes in the<i>Crtap<sup>-/-</sup></i>Mouse Model of Recessive Osteogenesis Imperfecta Open
Osteogenesis imperfecta (OI) is characterized by short stature, skeletal deformities, low bone mass with bone fragility, and motor deficits. A subset of OI patients also present with joint hypermobility; however, the role of tendon/ligamen…
View article: Sensing the load
Sensing the load Open
How does the skeleton detect and adapt to changes in the mechanical load it has to carry?
View article: Decision letter: Stimulation of Piezo1 by mechanical signals promotes bone anabolism
Decision letter: Stimulation of Piezo1 by mechanical signals promotes bone anabolism Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Mechanical loading, such as c…
View article: Decision letter: The mechanosensitive Piezo1 channel is required for bone formation
Decision letter: The mechanosensitive Piezo1 channel is required for bone formation Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Mechanical load of the skelet…
View article: Decision letter: Quantification of gene expression patterns to reveal the origins of abnormal morphogenesis
Decision letter: Quantification of gene expression patterns to reveal the origins of abnormal morphogenesis Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract The earliest developmental or…
View article: Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms
Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms Open
View article: Correction: Impaired Mitochondrial Energy Production Causes Light-Induced Photoreceptor Degeneration Independent of Oxidative Stress
Correction: Impaired Mitochondrial Energy Production Causes Light-Induced Photoreceptor Degeneration Independent of Oxidative Stress Open
[This corrects the article DOI: 10.1371/journal.pbio.1002197.].
View article: Loss of Frataxin induces iron toxicity, sphingolipid synthesis, and Pdk1/Mef2 activation, leading to neurodegeneration
Loss of Frataxin induces iron toxicity, sphingolipid synthesis, and Pdk1/Mef2 activation, leading to neurodegeneration Open
Mutations in Frataxin (FXN) cause Friedreich’s ataxia (FRDA), a recessive neurodegenerative disorder. Previous studies have proposed that loss of FXN causes mitochondrial dysfunction, which triggers elevated reactive oxygen species (ROS) a…
View article: Author response: Loss of Frataxin induces iron toxicity, sphingolipid synthesis, and Pdk1/Mef2 activation, leading to neurodegeneration
Author response: Loss of Frataxin induces iron toxicity, sphingolipid synthesis, and Pdk1/Mef2 activation, leading to neurodegeneration Open
Full text Figures and data Side by side Abstract eLife digest Introduction Results Discussion Materials and methods References Decision letter Author response Article and author information Metrics Abstract Mutations in Frataxin (FXN) caus…