Nemanja Vuksanovic
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View article: ActVI-ORFA directs metabolic flux towards actinorhodin by preventing intermediate degradation
ActVI-ORFA directs metabolic flux towards actinorhodin by preventing intermediate degradation Open
The biosynthetic pathway of actinorhodin in Streptomyces coelicolor A3(2) has been studied for decades as a model system of type II polyketide biosynthesis. The actinorhodin biosynthetic gene cluster includes a gene, actVI-orfA , that enco…
View article: Characterization of PglJ, a Glycosyltransferase in the <i>Campylobacter concisus</i> N-Linked Protein Glycosylation Pathway that Expands Glycan Diversity
Characterization of PglJ, a Glycosyltransferase in the <i>Campylobacter concisus</i> N-Linked Protein Glycosylation Pathway that Expands Glycan Diversity Open
The Campylobacter genus of Gram-negative bacteria is characterized by the expression of N-linked protein glycosylation (pgl) pathways. As Campylobacter concisus is an emerging human pathogen, a better understanding of the variation of the …
View article: Specificity determinants revealed by the structure of glycosyltransferase <i>Campylobacter concisus</i><scp>PglA</scp>
Specificity determinants revealed by the structure of glycosyltransferase <i>Campylobacter concisus</i><span>PglA</span> Open
In selected Campylobacter species, the biosynthesis of N‐linked glycoconjugates via the pgl pathway is essential for pathogenicity and survival. However, most of the membrane‐associated GT‐B fold glycosyltransferases responsible for divers…
View article: Synergistic computational and experimental studies of a phosphoglycosyl transferase membrane/ligand ensemble
Synergistic computational and experimental studies of a phosphoglycosyl transferase membrane/ligand ensemble Open
Complex glycans serve essential functions in all living systems. Many of these intricate and byzantine biomolecules are assembled employing biosynthetic pathways wherein the constituent enzymes are membrane-associated. A signature feature …
View article: Synergistic computational and experimental studies of a phosphoglycosyl transferase membrane/ligand ensemble
Synergistic computational and experimental studies of a phosphoglycosyl transferase membrane/ligand ensemble Open
Complex glycans serve important functions in all living systems. Many of these intricate and byzantine biomolecules are assembled employing biosynthetic pathways wherein the constituent enzymes are membrane associated. A signature feature …
View article: Oral and Inhaled Fosamprenavir Reverses Pepsin‐Induced Damage in a Laryngopharyngeal Reflux Mouse Model
Oral and Inhaled Fosamprenavir Reverses Pepsin‐Induced Damage in a Laryngopharyngeal Reflux Mouse Model Open
Objective More than 20% of the US population suffers from laryngopharyngeal reflux. Although dietary/lifestyle modifications and alginates provide benefit to some, there is no gold standard medical therapy. Increasing evidence suggests tha…
View article: Structural and Preliminary Biochemical Characterization of MppQ, a PLP-Dependent Aminotransferase from <i>Streptomyces hygroscopicus</i>
Structural and Preliminary Biochemical Characterization of MppQ, a PLP-Dependent Aminotransferase from <i>Streptomyces hygroscopicus</i> Open
MppQ is an enzyme of unknown function from Streptomyces hygroscopicus that is involved in the biosynthesis of the nonproteinogenic amino acid L-enduracididine (L-End). Since L-End is a component of several peptides showing high activity ag…
View article: Engineering a more specific <i>E. coli</i> glyoxylate/hydroxypyruvate reductase for coupled steady state kinetics assays
Engineering a more specific <i>E. coli</i> glyoxylate/hydroxypyruvate reductase for coupled steady state kinetics assays Open
The E. coli glyoxylate reductase/hydroxypyruvate reductase A (EcGhrA) was investigated as a coupling enzyme to monitor the transamination of 2-ketoarginine and glycine by the L-enduracididine biosynthetic enzyme MppQ. Surprisingly, 2-ketoa…
View article: Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis
Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis Open
Proteins belonging to the NTF2-like superfamily are present in the biosynthetic pathways of numerous polyketide natural products, such as anthracyclins and benzoisochromanequinones. Some have been found to be bona fide polyketide cyclases,…
View article: Discovery of Drug-like Ligands for the Mac1 Domain of SARS-CoV-2 Nsp3
Discovery of Drug-like Ligands for the Mac1 Domain of SARS-CoV-2 Nsp3 Open
Small molecules that bind the SARS-CoV-2 non-structural protein 3 Mac1 domain in place of ADP-ribose could be useful as molecular probes or scaffolds for COVID-19 antiviral drug discovery because Mac1 has been linked to coronavirus’ abilit…
View article: Molecular Basis for ADP-Ribose Binding to the Mac1 Domain of SARS-CoV-2 nsp3
Molecular Basis for ADP-Ribose Binding to the Mac1 Domain of SARS-CoV-2 nsp3 Open
The virus that causes COVID-19, SARS-CoV-2, has a large RNA genome that encodes numerous proteins that might be targets for antiviral drugs. Some of these proteins, such as the RNA-dependent RNA polymerase, helicase, and main protease, are…
View article: Molecular Basis for ADP-ribose Binding to the Macro-X Domain of SARS-CoV-2 Nsp3
Molecular Basis for ADP-ribose Binding to the Macro-X Domain of SARS-CoV-2 Nsp3 Open
The virus that causes COVID-19, SARS-CoV-2, has a large RNA genome that encodes numerous proteins that might be targets for antiviral drugs. Some of these proteins, such as the RNA-dependent RNA polymers, helicase and main protease, are we…
View article: <i>Streptomyces wadayamensis</i> MppP is a PLP-Dependent Oxidase, Not an Oxygenase
<i>Streptomyces wadayamensis</i> MppP is a PLP-Dependent Oxidase, Not an Oxygenase Open
The PLP-dependent l-arginine hydroxylase/deaminase MppP from Streptomyces wadayamensis (SwMppP) is involved in the biosynthesis of l-enduracididine, a nonproteinogenic amino acid found in several nonribosomally produced peptide antibiotics…