Nemat Sokhandan Bashir
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View article: Evidence suggestive of Cauliflower mosaic virus transcribing complementary DNA from Cucumber mosaic virus RNA in mixed infection
Evidence suggestive of Cauliflower mosaic virus transcribing complementary DNA from Cucumber mosaic virus RNA in mixed infection Open
The aim of this study was to investigate if Cauliflower mosaic virus (CaMV) reverse transcriptase (RT) can make complementary DNA (cDNA) from Cucumber mosaic virus (CMV) genomic RNAs in the mixed infection. First, symptoms of single infect…
View article: Development and Validation of a Histologic Respiratory Index (HRI) in Poultry
Development and Validation of a Histologic Respiratory Index (HRI) in Poultry Open
Respiratory viral diseases infecting poultry lead to variable lesions in the respiratory organs, including nasal sinuses, trachea, lungs, and air sacs. Additional involvement of eyelids/conjunctiva was reported. The distribution and the in…
View article: Development and Validation of a Histologic Respiratory Index (HRI) in Poultry
Development and Validation of a Histologic Respiratory Index (HRI) in Poultry Open
Viral respiratory diseases infecting poultry lead to variable lesions in the respiratory or-gans, including nasal sinuses, eyelids, trachea, lungs, and air sacs. The distribution and the intensity of lesions depend on multiple factors, inc…
View article: Association of satellite RNA with Grapevine fanleaf virus in its geographical origin and sequence characteristics
Association of satellite RNA with Grapevine fanleaf virus in its geographical origin and sequence characteristics Open
The region between The Caspian Sea and Black Sea has been hypothesized as the origin of grapevine. Likewise, an extensive study on Grapevine fanleaf virus (GFLV) from this region suggests this region as a possible origin of the virus. Howe…
View article: Production and Partial Purification of the Grapevine Fanleaf Virus Coat Protein 42 Polyclonal Antibody Against Inclusion Body Expressed in Escherichia coli
Production and Partial Purification of the Grapevine Fanleaf Virus Coat Protein 42 Polyclonal Antibody Against Inclusion Body Expressed in Escherichia coli Open
It was evident that the recombinant coat protein in the form of inclusion bodies can be prepared and used as the antigen for immunizing animals in order to produce PAbs.
View article: Detection of Russian olive witches’-broom disease and its insect vector in Northwestern Iran
Detection of Russian olive witches’-broom disease and its insect vector in Northwestern Iran Open
Recently, Russian olive trees showing witches’-broom and little leaf symptoms have been widely observed in northwestern and central Iran. Polymerase chain reaction (PCR) and nested PCR assays using phytoplasma universal primer pairs confir…
View article: Extraction and molecular detection of viral dsRNA from different infected plants
Extraction and molecular detection of viral dsRNA from different infected plants Open
Extraction of viral double stranded RNA (dsRNA) from infected plants is helpful in identification of the viruses involved in infection. To date, there have been several methods developed to isolate dsRNA; however, type of the plant and vir…
View article: Molecular detection of Grapevine fanleaf Virus by the isolation of ssRNA and dsRNA from Xiphinema index
Molecular detection of Grapevine fanleaf Virus by the isolation of ssRNA and dsRNA from Xiphinema index Open
Xiphinema index is an important grapevine pathogen nematode which also vectors Grapevine fanleaf virus. The viral genes involved in transmission by the vector nematode are mapped to the C-terminal residues of RNA2-encoded polyprotein. To r…
View article: Preparation of polyclonal antibody against recombinant coat protein of Cucumber mosaic virus isolate B13
Preparation of polyclonal antibody against recombinant coat protein of Cucumber mosaic virus isolate B13 Open
View article: Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli
Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli Open
The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide ran…
View article: Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187
Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187 Open
The full-length cDNA of PVY-CP was amplified from the infected potato leaves. The cDNA was heterologously expressed in E. coli. The produced recombinant CP can be used as an antigen to generate polyclonal antibody.