Nicholas E. Lea
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View article: Kinase condensates enrich ATP and trigger autophosphorylation
Kinase condensates enrich ATP and trigger autophosphorylation Open
Kinase-mediated signal transduction regulates most cellular processes, and autophosphorylation is a common mechanism to activate kinases. Many kinases undergo phase separation to form condensates. Despite the central role of autophosphoryl…
View article: Publisher Correction: Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses
Publisher Correction: Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses Open
View article: Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses
Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses Open
Rabies-virus-based monosynaptic tracing is a widely used technique for mapping neural circuitry, but its cytotoxicity has confined it primarily to anatomical applications. Here we present a second-generation system for labeling direct inpu…
View article: Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency
Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency Open
Rabies viral vectors have become important components of the systems neuroscience toolkit, allowing both direct retrograde targeting of projection neurons and monosynaptic tracing of inputs to defined postsynaptic populations, but the rapi…
View article: “Self-inactivating” rabies viruses are susceptible to loss of their intended attenuating modification
“Self-inactivating” rabies viruses are susceptible to loss of their intended attenuating modification Open
Monosynaptic tracing using rabies virus is an important technique in neuroscience, allowing brain-wide labeling of neurons directly presynaptic to a targeted neuronal population. A 2017 article reported the development of a noncytotoxic ve…
View article: 341 Repeats Is Not Enough for Methylation in a New Fragile X Mouse Model
341 Repeats Is Not Enough for Methylation in a New Fragile X Mouse Model Open
Fragile X syndrome (FXS) is a leading monogenic cause of intellectual disability and autism spectrum disorders, spurring decades of intense research and a multitude of mouse models. So far, these models do not recapitulate the genetic unde…
View article: Targeting thalamic circuits rescues motor and mood deficits in PD mice
Targeting thalamic circuits rescues motor and mood deficits in PD mice Open
View article: Single-deletion-mutant, third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency
Single-deletion-mutant, third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency Open
SUMMARY Rabies viral vectors have become important components of the systems neuroscience toolkit, allowing both direct retrograde targeting of projection neurons and monosynaptic tracing of inputs to defined postsynaptic populations, but …
View article: Long-term labeling and imaging of synaptically-connected neuronal networks<i>in vivo</i>using double-deletion-mutant rabies viruses
Long-term labeling and imaging of synaptically-connected neuronal networks<i>in vivo</i>using double-deletion-mutant rabies viruses Open
Monosynaptic tracing is a widely-used technique for mapping neural circuitry, but its cytotoxicity has confined it primarily to anatomical applications. Here we present a second-generation system for labeling direct inputs to targeted neur…
View article: Anterior thalamic dysfunction underlies cognitive deficits in a subset of neuropsychiatric disease models
Anterior thalamic dysfunction underlies cognitive deficits in a subset of neuropsychiatric disease models Open
View article: Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations
Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations Open
Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus (RV) has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to a starting …
View article: Monosynaptic tracing success depends critically on helper virus concentrations
Monosynaptic tracing success depends critically on helper virus concentrations Open
Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to a starting neuro…
View article: Nontoxic, double-deletion-mutant rabies viral vectors for retrograde targeting of projection neurons
Nontoxic, double-deletion-mutant rabies viral vectors for retrograde targeting of projection neurons Open
View article: <i>In Vivo</i> Behavior of the Tandem Glycine Riboswitch in <i>Bacillus subtilis</i>
<i>In Vivo</i> Behavior of the Tandem Glycine Riboswitch in <i>Bacillus subtilis</i> Open
In many bacterial species, the glycine riboswitch is composed of two homologous ligand-binding domains (aptamers) that each bind glycine and act together to regulate the expression of glycine metabolic and transport genes. While the struct…